FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1042591
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTACCTTGCTCGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAGTCAGT58270.5588960579939785No Hit
GGTACACACTGGCCGCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT44370.4255743623338394No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42820.41070755454439944No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA40040.3840432154123717No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCT36690.3519117276093885No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATATAGTGAGTTCTTCGCGTTGTCTCTGGA33400.3203557291401901No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA33020.3167109633595533No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27970.2682739444326682No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT27080.2597375193148608No Hit
GGGTTAGGCACTGACTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCGAGCAA24910.2389239884096448No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC24310.2331690950717971No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA22610.2168635639478952No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21900.21005360683144206No Hit
GTGTATTACTGTGCGAGGGACCCCTTCTATGGCTCTGTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC21680.2079434792742312No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC21010.2015171817136346No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC20700.1985438201557466No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20660.19816016059989008No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC19830.19019922481586737No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA19620.1881850121476207No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAG19380.1858830548124816No Hit
AGGTACACACTGGCCGCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT19150.18367701236630662No Hit
GGCCAAAAGTGTGCGGCCAGTGTGTACCTTGCTCGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCT19070.1829096932545936No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18770.18003224658566974No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAGTCAGTGCCTAACCCACTGCC17560.16842654502101015No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17550.16833063013204602No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA17370.1666041621306917No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17110.16411037501762438No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC17050.1635348856838396No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16650.15969829012527442No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT16510.15835548167977664No Hit
GTCTGGGACCCCAGAGTCCCGGTTAAAAACGTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA15830.15183326923021587No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA15260.14636612055926054No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15190.14569471633651163No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTTAGGCACTGACTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGAT14910.14300909944551604No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG14260.13677463166284765No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14170.1359113976621705No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTAAA14100.13523999343942159No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCA14030.1345685892166727No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT13880.13312986588221076No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC13720.1315952276587847No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGAT13680.13121156810292817No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13560.13006058943535864No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAACGTTTTTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCG13500.12948510010157385No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTAAACGGTGATG13090.1255525896540446No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12490.11979769631619687No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC12420.11912629209344795No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT12370.11864671764862732No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12320.11816714320380668No Hit
GCCTAACCCACTGCCGCTGAATCTGTCTGGGACCCCAGAGTCCCGGTTAAAAACGTTATAAATTAGGCGCCTTG12250.11749573898105777No Hit
GGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAACGTTTTTAACCGGGACTCTGGGGTCCCA12190.11692024964727299No Hit
GTACACACTGGCCGCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA12180.11682433475830886No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGCTCTTGGATGGGCTGGGTCCGCCAGGCTCCAGGGAAAGG11690.11212450519906655No Hit
GGTCTAGTCAAAGCCTCGTAAACGGTGATGGAAACACGTACTTGCATTGGTTTCAGCAGAGGCCAGGCCAATCTC11650.11174084564321003No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11540.11068578186460463No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATATAGTGAGTTCTTCGCGTTGTCTCTG11520.11049395208667637No Hit
GGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCGAGCAAGGTACACACTGGCCGCACACTTTTGGCCAGGG11490.11020620741978397No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11490.11020620741978397No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11410.10943888830807094No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11300.10838382452946554No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11220.10761650541775251No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG11160.10704101608396772No Hit
GGCCTGGCCTCTGCTGAAACCAATGCAAGTACGTGTTTCCATCACCGTTTACGAGGCTTTGACTAGACCTGCAG10950.10502680341572103No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10890.10445131408193627No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC10890.10445131408193627No Hit
GTATTACTGTGCGAGGGACCCCTTCTATGGCTCTGTTGACTACTGGGGCC10830.10387582474815148No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10540.10109429296819175No Hit
GATCAGCACTGCGCACAGAGGACTCACCATGGAGTTGGGGCTGAACTGGG10430.10003922918958633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA3100.080.95222570
ACTTGCA17450.070.2140970
TGGACTA700.003319236263.26518670
TAGGCAA3150.060.92203570
AGAGCAT9550.055.64686670
TATTCTA1902.714296E-854.38586470
CTTAACG200.00790462350.82185430
GAGATTA454.4173384E-449.4492469
ATGGCAA2157.188464E-848.06192470
ATTATAT1603.3522014E-546.13086770
ACCCGGT1306.7070144E-445.4211670
GGTATCA8200.040.290041
GTAGTTT2204.6403093E-640.25966670
CGGCAAA1857.895093E-539.8969770
GGAATCA1500.001356381139.36500570
TTCTTAA2653.715868E-738.9936470
GAGCAAA22950.038.59314370
GTATCAA20750.037.5300671
CAGCCAT9050.037.51637370
AGTCAGT21100.037.17481669
Produced by FastQC (version 0.11.5)