FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1228773
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACTGCCGTGTCGTCGGCCCTCAGGCTGTACATTTGCAGATAAA63690.5183219357847219No Hit
GATTATTACTGTGGGTCATGGGATGACAGCCTGAATGGTTTCGTCTTCGGAAGTGCGACGAAGGTCACCGTCC32350.2632707587162153No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG31110.2531793911487313No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAG29840.24284387759171142No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC28910.2352753519160984No Hit
GTATATTACTGTGCGAGAGATTGGAGGCCGACTGCGGTGACATATTATTT28630.2329966560137633No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCTTCGTCGCA28040.22819511821955724No Hit
GATTAGTACTATAGATGAGGAGTTTGGGGGCCTTTCCTGGGACTTGCTGATACCAATAAACAATGTTGTATCCGA28030.22811373622304526No Hit
GTCCTGGGCCCAGTCTATGCTGACTCAGCCACCCTCAGTATCTGGGACCCCCGGGCAGAGGGTCACCATCTCT27870.22681162427885376No Hit
GTACTAATCAGCGGCCCTCGGGGGTCCCTGACCGATTCTCCGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCA27440.2233121984288392No Hit
GTACTATAGATGAGGAGTTTGGGGGCCTTTCCTGGGACTTGCTGATACCA26960.2194058625962647No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG25550.20793100108807727No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC23350.19002696185544443No Hit
CTATAGTACTAATCAGCGGCCCTCGGGGGTCCCTGACCGATTCTCCGGCTCCAAGTCTGGCACCTCAGCCTCC23080.1878296479496213No Hit
GCCTTGGGCTGACCCAGGACGGTGACCTTCGTCGCACTTCCGAAGACGAAACCATTCAGGCTGTCATCCCATGAC22660.18441160409611865No Hit
GTAATAATCAGCCTCATCTTCAGACTGGAGACCACTGATGGCCAGGGAGG22530.18335363814146308No Hit
GTGCAGGGTCCTGGGCCCAGTCTATGCTGACTCAGCCACCCTCAGTATCTGGGACCCCCGGGCAGAGGGTCACCA21920.17838933635423304No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT21780.1772499884030655No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC21200.17252983260537136No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC20100.16357781298905494No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19620.15967147715648047No Hit
GTCTATGCTGACTCAGCCACCCTCAGTATCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGCTCTGGAGGCGG19300.15706725326809753No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC19060.15511408535181032No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18970.1543816473832026No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCTTCGTC18210.14819661564829306No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17790.1447785717947904No Hit
CTCCTGTGCAGCGTCTGGATTCAGTTTCAGTAGTTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG17320.14095361795872793No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT17210.14005841599709629No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG17000.13834939407034497No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG17000.13834939407034497No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16370.133222328290091No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA16300.13265265431450723No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15740.12809526250983705No Hit
GTATATTACTGTGCGAGAGATTGGAGGCCGACTGCGGTGACATATTATTTCGGAATGGACGTCTGGGGCCAAGGG15530.12638624058308573No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT15460.12581656660750196No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG15420.12549103862145408No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15390.12524689263191818No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTATGCTGACTCAGCCAC15250.12410754468075062No Hit
ACCCAGGACGGTGACCTTCGTCGCACTTCCGAAGACGAAACCATTCAGGC14820.12060811883073602No Hit
CAGTAATAATCAGCCTCATCTTCAGACTGGAGACCACTGATGGCCAGGGA14480.11784113094932913No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14310.11645763700862569No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGC14130.11499276107141025No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG14040.11426032310280256No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG13880.11295821115861107No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAG13850.11271406516907517No Hit
GTATCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGCTCTGGAGGCGGCTCTAACATCGGATACAACATTGTT13730.11173748121093155No Hit
CAGTAATATACTGCCGTGTCGTCGGCCCTCAGGCTGTACATTTGCAGATAAAGCGTGTCGTTGGAATTGTCTC13730.11173748121093155No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13720.1116560992144196No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC13710.11157471721790761No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13670.11124918923185974No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG13130.1068545614202135No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC13050.10620350544811777No Hit
GGGCTGGAGTGGGTGGCGTTCACATGGTATGATGGAAGTAGAAAATATTA12950.10538968548299808No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC12870.10473862951090233No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC12790.10408757353880659No Hit
TTATTACTGTGGGTCATGGGATGACAGCCTGAATGGTTTCGTCTTCGGAAGTGCGACGAAGGTCACCGTCCTGGG12690.10327375357368693No Hit
ATATTACTGTGCGAGAGATTGGAGGCCGACTGCGGTGACATATTATTTCGGAATGGACGTCTGGGGCCAAGGGAC12580.10237855161205527No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12530.10197164162949544No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12410.10099505767135182No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG12410.10099505767135182No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)