FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004685253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1228773
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACTGCCGTGTCGTCGGCCCTCAGGCTGTACATTTGCAGATAAAGCGTGTCGTTGGAATTGTCTCTGGA57650.46916720989149335No Hit
GTATATTACTGTGCGAGAGATTGGAGGCCGACTGCGGTGACATATTATTTCGGAATGGACGTCTGGGGCCAAGG41290.3360262635979144No Hit
GATTATTACTGTGGGTCATGGGATGACAGCCTGAATGGTTTCGTCTTCGGAAGTGCGACGAAGGTCACCGTCCTG29330.23869339576960105No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC29010.23608917188121809No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28710.23364771198585904No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT28420.231287634087012No Hit
GTCCTGGGCCCAGTCTATGCTGACTCAGCCACCCTCAGTATCTGGGACCCCCGGGCAGAGGGTCACCATCTCTT28290.2302296681323564No Hit
GTACTAATCAGCGGCCCTCGGGGGTCCCTGACCGATTCTCCGGCTCCAAG26450.2152553807741544No Hit
GTACTATAGATGAGGAGTTTGGGGGCCTTTCCTGGGACTTGCTGATACCAATAAACAATGTTGTATCCGATGTTA26180.21305806686833126No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGT26170.21297668487181928No Hit
GATTAGTACTATAGATGAGGAGTTTGGGGGCCTTTCCTGGGACTTGCTGATACCAATAAACAATGTTGTATCC25210.20516401320667038No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCTTCGTCGCA24740.2013390593706079No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC23640.19238703975429147No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGG22910.18644615400891784No Hit
CTATAGTACTAATCAGCGGCCCTCGGGGGTCCCTGACCGATTCTCCGGCTCCAAGTCTGGCACCTCAGCCTCC21450.17456438251817055No Hit
GTGCAGGGTCCTGGGCCCAGTCTATGCTGACTCAGCCACCCTCAGTATCT20840.16960008073094054No Hit
GCCTTGGGCTGACCCAGGACGGTGACCTTCGTCGCACTTCCGAAGACGAAACCATTCAGGCTGTCATCCCATGAC20470.16658894685999776No Hit
GTAATAATCAGCCTCATCTTCAGACTGGAGACCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCGGA19850.16154326307625574No Hit
GTCTATGCTGACTCAGCCACCCTCAGTATCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGCTCTGGAGGCG18980.15446302937971457No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG18300.14892905361690076No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC17880.14551100976339812No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC17850.14526686377386222No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17780.14469718979827845No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17680.14388336983315878No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17650.14363922384362288No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCTTCGTCGCACTTCCGAAGACGAAACCATTCA17580.14306954986803908No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTATGCTGACTCAGCCACCCTCAGTATCTGGGACCCCCGGGCA17100.13916321403546464No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17040.13867492205639284No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG16880.13737281011220137No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTA16120.1311877783772918No Hit
CTCCTGTGCAGCGTCTGGATTCAGTTTCAGTAGTTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGG15990.13012981242263624No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC15650.12736282454122932No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15470.12589794860401393No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGT15370.12508412863889423No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCA15190.12361925270167884No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15130.12313096072260703No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAACTACTGAAACTGAATCCAGACGCTGCACA14980.12191023077492752No Hit
ACCCAGGACGGTGACCTTCGTCGCACTTCCGAAGACGAAACCATTCAGGC14640.1191432428935206No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT14220.11572519904001796No Hit
GTATCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGCTCTGGAGGCGG14110.11482999707838631No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG13940.11344650313768287No Hit
GTATGATGGAAGTAGAAAATATTATGCAGATTCTGTGAGGGGCCGATTCAGCATCTCCAGAGACAATTCCAAC13720.1116560992144196No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13720.1116560992144196No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTG13430.10929602131557253No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG13190.10734285339928529No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13160.1070987074097494No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCT13130.1068545614202135No Hit
GGGCTGGAGTGGGTGGCGTTCACATGGTATGATGGAAGTAGAAAATATTA13000.1057965954655579No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12990.10571521346904596No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC12960.10547106747951004No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG12860.10465724751439037No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT12820.1043317195283425No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC12720.10351789956322283No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGG12720.10351789956322283No Hit
CAGTAATATACTGCCGTGTCGTCGGCCCTCAGGCTGTACATTTGCAGATAAAGCGTGTCGTTGGAATTGTCTCTG12640.10286684359112708No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)