FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1176964
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTATTCGGCGGAGGGACCAAGCTGACCGTCCCAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCC52760.4482719947254122No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT40030.34011235687752556No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31090.26415421372276465No Hit
GCCTTGGGCTGACCTGGGACGGTCAGCTTGGTCCCTCCGCCGAATAAGACAGTGCTGCTGGTTGTATATGAGCT29440.2501350933418524No Hit
GAATAAGACAGTGCTGCTGGTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGAT29240.24843580602295395No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27850.2366257591566097No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCT26680.22668492834105378No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23140.19660754279655113No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA22770.193463861256589No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22510.19125478774202098No Hit
GACCTGGGACGGTCAGCTTGGTCCCTCCGCCGAATAAGACAGTGCTGCTGGTTGTATATGAGCTGCAGTAATAAT22460.1908299659122964No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21840.18556217522371118No Hit
TCCCAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG21780.18505238902804164No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTGGGACGGTCAGCTTGGTCCCTC21340.18131395692646504No Hit
GATTATTACTGCAGCTCATATACAACCAGCAGCACTGTCTTATTCGGCGGAGGGACCAAGCTGACCGTCCCAGGT21230.18037934890107088No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT20740.17621609496976967No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATA20430.17358219962547708No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC19610.1666151216179934No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18470.15692918390027225No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA18280.1553148609473187No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAACCAGCAGCACTGTCTTATTCG17580.14936735533117412No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17470.14843274730577996No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC17300.14698835308471625No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17000.1444394221063686No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG16780.1425702060555803No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTG16450.13976638197939784No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG16080.1366227004394357No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTT15830.13449859129081265No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCT15760.13390384072919817No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG15500.1316947672146302No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT15280.12982555116384187No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15240.1294856937000622No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14960.12710669145360437No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14400.1223486869606887No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG14240.12098925710556992No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT13990.11886514795694686No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13890.11801550429749762No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGACAGACTTGGAGCCAGA13880.11793053993155271No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13830.11750571810182808No Hit
GTGCTGCTGGTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAG13780.11708089627210348No Hit
ATAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGAGTTCTTGGCGTTGTCTCTGGA13560.11521168022131517No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13420.11402217909808626No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCA13290.11291764234080226No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG13160.11181310558351827No Hit
GTCTATTATTGTGCGAGAGATCAAGGTCGGTTTACGTTTAGCAGTGGCTCCTTTGACTACTGGGGCCAGGGAACC12640.10739495855438229No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12530.10646035052898814No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12320.10467609884414478No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC12250.1040813482825303No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGCGT150.002801412197.7648370
GCGGGTT750.00428140159.32945370
GCGACGT850.007025546452.34951470
TGACAGT21850.044.12373470
AATGGTA2401.6455851E-743.2610670
GTGGTAT4300.041.6773531
GGTATCA12600.039.717681
TGAATCA1500.001325289439.55296770
AAGCGGT13550.039.40701770
CAGCCAT13250.039.1798370
GGTAGAA21250.038.38964570
TGGTATC4850.037.614652
GTATCAA31500.037.570781
TCTCGAC450.001917303336.6984268
TTTAAGT2459.405065E-636.32415470
CCGCTAT1650.002116541135.95724570
TTCTTAA2551.2371651E-534.89967770
GTAGTTT2151.8595642E-434.4938770
TATTCTA2651.6099604E-533.5827170
AGTGGAA15850.031.81705770
Produced by FastQC (version 0.11.5)