FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1111574
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGAGCTTGTATATGAGCTGCAGTAATAATCAGCTTCGTCCTCAGC52390.4713136507331046No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG47280.42534280218860826No Hit
CTCCAGTACTGTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA44430.39970348352876195No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41710.3752336776498911No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCC41480.3731645396527807No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCACAGTACTGGAGCTTGTATATGAGCTGCAGTAATAA39350.354002522549106No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCACAGTACTGGAGCTTGTATATGAGC38330.3448263453445295No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG34280.30839152409106363No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC31860.2866205938605977No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG29990.26979760231887395No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT29280.2634102632843157No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT29200.26269056311140776No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC27190.24460809626709515No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26380.23732113201640195No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26240.23606165671381302No Hit
GATTATTACTGCAGCTCATATACAAGCTCCAGTACTGTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAGG25960.23354270610863515No Hit
TCTCTGGGCTCCAGGCTGAGGACGAAGCTGATTATTACTGCAGCTCATAT25040.22526615412019352No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25020.22508622907696654No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCG24160.2173494522182059No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCGCCCAGGCAAAGC24050.21635986448045744No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG23890.21492046413464153No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23540.21177177587816914No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22720.2043948491058625No Hit
CCACAGTACTGGAGCTTGTATATGAGCTGCAGTAATAATCAGCTTCGTCC22230.19998668554680119No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22190.19962683546034724No Hit
GTACTGTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGT21790.1960283345958074No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21610.19440900920676446No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC21190.19063058329899762No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20940.18838152025866026No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGT20920.18820159521543325No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20340.18298376896185048No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19780.1779458677514947No Hit
GGTACCAACAGCGCCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTC19640.17668639244890577No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG19470.17515702958147636No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC19460.17506706705986286No Hit
GTACCAACAGCGCCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAACCGGCCCTCAGGGGTTTCTAA18740.16858976550369117No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC18680.1680499903740102No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18280.16445148950947036No Hit
GTAATAATCAGCTTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG18250.16418160194462986No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAG17970.16166265133945198No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17320.15581508743457476No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17000.15293628674294288No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT16980.15275636169971588No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCGCCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAC16700.150237411094538No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAG16590.14924782335678957No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCACAGTACTGGAGC16500.14843816066226811No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGG16010.1440299971032068No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTG15830.14241067171416388No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCACAGTAC15730.1415110464980289No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCACAG15460.13908205841446453No Hit
GGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAACCGGCCCTCAGG15370.13827239571994307No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15310.1377326205902621No Hit
CTGTTGGTACCAGGAGACATAGTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGA15310.1377326205902621No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14840.13350438207442777No Hit
GTTGGTACCAGGAGACATAGTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGACTGTC14340.12900625599375298No Hit
GTCTCTGGTTACTCCATCAGCAGTGGTTTCTCCTGGGGCTGGATCCGGCAGGCCCCAGGGAAGGGGCTGGAG14330.1289162934721395No Hit
GTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG14240.12810663077761802No Hit
GTATATGAGCTGCAGTAATAATCAGCTTCGTCCTCAGCCTGGAGCCCAGA14090.12675719295341561No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATTAGAAACCCCTGAGGGCCGGT13880.12486797999953218No Hit
GTATCAACGCAGAGTACGGGGCTGGGGTCTCAGGAGGCAGCGCTCTCAGGACATCTCCACCATGGCCTGGGC13810.12423824234823772No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13750.12369846721855673No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT13640.12270887948080829No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG13580.12216910435112732No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG13330.11992004131078993No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13270.11938026618110896No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13250.11920034113788196No Hit
GAATACCACAGTACTGGAGCTTGTATATGAGCTGCAGTAATAATCAGCTTCGTCCTCAGCCTGGAGCCCAGAGA13210.11884049105142798No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCC13100.11785090331367953No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13070.11758101574883904No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13030.11722116566238507No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCG12960.11659142801109057No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA12870.11578176531656913No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCT12860.11569180279495561No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC12830.11542191523011512No Hit
GTCAGTAACCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCA12720.11443232749236668No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC12570.11308288966816425No Hit
ATCTATCATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGT12560.11299292714655074No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12550.11290296462493725No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTG12460.11209330193041578No Hit
GTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCG12100.10885465115232994No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12050.10840483854426247No Hit
GGATAGCACACACCACCAGGACAATCTCCTCCCCCTCTATCTCTCGCACA12040.10831487602264896No Hit
CTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGA12000.10795502593619498No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12000.10795502593619498No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGG11780.10597585046069809No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11420.10273719968261223No Hit
GTTACTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGCGCTGTT11410.10264723716099872No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11370.10228738707454474No Hit
GAAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTGAGTTTCAGGGAGAACTGGTTGTTGGACGTGTCTAGTGA11330.10192753698809076No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11320.10183757446647727No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATT11140.10021824907743433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2800.0102.0579570
ACACGTT150.002462357368.2324136
CCGTAGT252.8008764E-454.234711
AATGGTA2003.6379788E-1252.91893870
TGGTATC2850.047.5614622
CGTATGC2100.045.73473441
TCGTATG2250.044.17202840
AAAACCG1204.3780896E-544.09911370
AGACGTA2600.043.0324125
GTATGCC2350.042.38105842
GGTATCA9200.042.3708651
CGTAGAG2650.042.26993628
TATACAC2650.042.201483
ACGTAGA2800.041.2066227
TCTCGTA2250.041.015638
ACACATC2850.040.429066
CCGTCTT2350.039.64982247
GTATCAA23900.039.427951
GACGTAG2850.039.27000426
CGAGACG2850.039.24703623
Produced by FastQC (version 0.11.5)