FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1111574
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGAGCTTGTATATGAGCTGCAGTAATAATCAGCTTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAG47260.42516287714538126No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT46360.41706625020016663No Hit
CTCCAGTACTGTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA44080.3965547952722896No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41580.3740641648689156No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCT39730.3574210983704189No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCACAGTACTGGAGCTTGTATATGAGCT36350.32701376606505733No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCACAGTACTGGAGCTTGTATATGAGCTGCAGTAATAAT34730.312439837563671No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33890.3048829857481373No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC29350.2640400009356102No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT28040.25225491060424227No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC27970.25162517295294784No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG27450.24694712182904605No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26250.2361516192354265No Hit
GATTATTACTGCAGCTCATATACAAGCTCCAGTACTGTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAGGT25590.2302140928089358No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25450.22895461750634685No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATA24190.21761933978304637No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23910.2151003891778685No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA23710.21330113874559858No Hit
TCTCTGGGCTCCAGGCTGAGGACGAAGCTGATTATTACTGCAGCTCATATACAAGCTCCAGTACTGTGGTATTCG23660.2128513261375311No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCGCCCAGGCAAAGC23590.21222158848623662No Hit
GTACTGTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCC23400.2105123005755802No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGC23230.2089829377081508No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG22430.2017859359790711No Hit
CCACAGTACTGGAGCTTGTATATGAGCTGCAGTAATAATCAGCTTCGTCCTCAGCCTGGAGCCCAGAGATGGTCA22230.19998668554680119No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21340.1919800211232001No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21320.1918000960799731No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21270.1913502834719056No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG21150.19027073321254365No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT20730.1864923073047768No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG20490.18433320678605292No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA19610.1764165048840653No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTG19420.17470721697340888No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT19250.17317785410597944No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCT19140.172188266368231No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18980.17074886602241507No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC18700.1682299154172372No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG18620.1675102152443292No Hit
GGTACCAACAGCGCCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTC18220.16391171437978938No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC17880.16085298864493053No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG17280.15545523734812078No Hit
GTACCAACAGCGCCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCA17100.15383591195907784No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGT16270.1463690226651577No Hit
GTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCC16240.14609913510031722No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCGCCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAC16120.14501958484095526No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15890.14295044684384484No Hit
GTAATAATCAGCTTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGA15800.1421407841493234No Hit
GTCTCTGGTTACTCCATCAGCAGTGGTTTCTCCTGGGGCTGGATCCGGCAGGCCCCAGGGAAGGGGCTGGAGTG15450.13899209589285103No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGA15440.13890213337123755No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15320.1378225831118756No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAAC14890.13395419468249528No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT14790.13305456946636032No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCACAGTACTGGAGC14580.1311653565124769No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCACAGTACTG14270.12837651834245853No Hit
GGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAACCGGCCCTCAGG14210.12783674321277755No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT14210.12783674321277755No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC14200.12774678069116405No Hit
CTGTTGGTACCAGGAGACATAGTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATC13870.1247780174779187No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13760.12378842974017025No Hit
GTATATGAGCTGCAGTAATAATCAGCTTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCA13750.12369846721855673No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13660.12288880452403529No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT13610.1224389919159678No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCA13350.12009996635401692No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT13130.11812079087852002No Hit
ATCTATCATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGT13100.11785090331367953No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG12890.1159616903597961No Hit
GTTGGTACCAGGAGACATAGTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGACTGTC12820.11533195270850163No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12750.11470221505720717No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTAT12610.11344273975461823No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA12590.11326281471139123No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAG12580.11317285218977774No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG12570.11308288966816425No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12500.11245315201686976No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA12330.11092378914944034No Hit
GTCAGTAACCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCAT12320.11083382662782684No Hit
GTATCAACGCAGAGTACGGGGCTGGGGTCTCAGGAGGCAGCGCTCTCAGGACATCTCCACCATGGCCTGGGCT12240.11011412645491889No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC12240.11011412645491889No Hit
GAATACCACAGTACTGGAGCTTGTATATGAGCTGCAGTAATAATCAGCTTCGTCCTCAGCCTGGAGCCCAGAGAT11970.10768513837135449No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTG11900.10705540072006002No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA11870.10678551315521953No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11520.10363682489874719No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATTAGAAACCCCTGAG11370.10228738707454474No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAA11350.10210746203131776No Hit
GTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGA11220.10093794925034232No Hit
CTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTC11170.10048813664227482No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA11130.10012828655582084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA2551.8189894E-1256.57225870
GGTAGAA28900.052.911770
CAGCCAT9550.046.82761470
TATTCTA1256.19354E-446.16296870
TGACAGT26050.042.08715470
AGTGGAA16200.040.9625170
AAGTTAT2154.643014E-640.25839670
TTCAAAA1450.001285960139.7956670
GTATCAA21200.039.254561
GGTATCA8100.038.423211
TGGTATC3450.037.2359282
CATGGTA2051.6547256E-435.1851970
TCCAACA11250.034.62222770
GCACCGT12150.034.4322570
CTCGTAT2101.4701081E-534.34744370
TATCAAC24300.034.2236632
TTTCTAA22900.033.38751670
TCTGAGT1750.003236521532.97354570
TTACTTT2202.5054335E-432.78619470
ATCAACG25700.032.4879573
Produced by FastQC (version 0.11.5)