FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700211
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT30480.4352973603670893No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCTTGAGGGAGAACTGGTTCTTGGACGTGTCTACTGA27080.3867405682001568No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25460.3636046848735595No Hit
GTATTACTGTCAACAACTTAATATTTTCCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTG22380.319617943734103No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT19180.273917433459343No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18770.2680620555803893No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT18420.26306356226908745No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGG18130.25892195352543734No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17850.25492315887639583No Hit
GATCTATGGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGATTCAGCGGTAGTAGATCTGGGACAGAATTCG16720.2387851661856212No Hit
GTCTTCAGGCTGCAGGCTGCTGATTGTGAGAGCGAATTCTGTCCCAGATCTACTACCGCTGAATCTTGATGGGAC16130.23035913460371232No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC15840.22621752586006216No Hit
GTCTCCATCCTTCCTGTCTGCATCTATAGGAGACAGAATCACCATCACTTGCCGGGCCAGTCAGGCCATTAGCAG15780.22536064129241043No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15760.2250750131031932No Hit
CAGTAATACGTTGCAAAGTCTTCAGGCTGCAGGCTGCTGATTGTGAGAGCGAATTCTGTCCCAGATCTACTACCG15400.21993370569728266No Hit
GTCTATTACTGTGCGAGAGTAAACGAGGAGGGGCTGCCCCGTTCCTACTA15360.21936244931884818No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15020.2145067701021549No Hit
CCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTGTTTGCTGATACCAGGCTAAAGAACTGCTAATGGCCTGAC14610.20865139222320128No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14410.2057951103310288No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14280.20393852710111665No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14130.20179631568198728No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13860.1979403351275544No Hit
GTAGTAGATCTGGGACAGAATTCGCTCTCACAATCAGCAGCCTGCAGCCT13830.19751189284372855No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13800.19708345055990265No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13560.19365591228929566No Hit
CACCTGTACTGTCTCTGATGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGA13210.18865741897799376No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC12970.18522988070738677No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12930.18465862432895228No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACA12780.1825164129098229No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT12320.17594696455782616No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC12300.17566133636860887No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11670.1666640484082655No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATG11420.16309369604304988No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC11260.16080867052931186No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11260.16080867052931186No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11260.16080867052931186No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11160.15938052958322563No Hit
GTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGATTCAGCGGTAGTAGATCTGGGACAGAATTCGCTCTCACAA11160.15938052958322563No Hit
GTCTCTGATGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGAT10990.156952689974879No Hit
GTGGATGCACCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTGTTTGCTGATACCAGGCTAAAGAACTGCTAA10680.15252545304201162No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10650.15209701075818574No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT10100.14424223555471138No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9990.1426712805140165No Hit
GTAATACGTTGCAAAGTCTTCAGGCTGCAGGCTGCTGATTGTGAGAGCGAATTCTGTCCCAGATCTACTACCG9710.138672485864975No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC9610.13724434491888873No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9430.13467369121593348No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9310.13295992208062998No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9290.13267429389141272No Hit
GCCTGAAGACTTTGCAACGTATTACTGTCAACAACTTAATATTTTCCCTTGGACGTTCGGCCAAGGGACCAAGG9280.1325314797968041No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG9130.13038926837767473No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8980.12824705695854535No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8930.1275329864855022No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8890.12696173010706774No Hit
ACTTAATATTTTCCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCA8860.12653328782324186No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8780.12539077506637286No Hit
CACCAGGGAAAGCCCCTAACCTCCTGATCTATGGTGCATCCACTTTGCAA8760.1251051468771556No Hit
GGCCATTAGCAGTTCTTTAGCCTGGTATCAGCAAACACCAGGGAAAGCCCCTAACCTCCTGATCTATGGTGCATC8660.12367700593106934No Hit
ACGTATTACTGTCAACAACTTAATATTTTCCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA8600.1228201213634176No Hit
GCTAATGGCCTGACTGGCCCGGCAAGTGATGGTGAT8590.12267730726880897No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8540.12196323679576584No Hit
GGTTAGGGGCTTTCCCTGGTGTTTGCTGATACCAGGCTAAAGAACTGCTAATGGCCTGACTGGCCCGGCAAGTGA8350.11924976899820197No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA8270.11810725624133298No Hit
ACCTACTACAATCCCTCCCTCAAAGGTCGAGTCTCCATGTCAGTAGACACGTCCAAGAACCAGTTCTCCCTCAAG8260.11796444214672433No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8190.11696474348446396No Hit
ATCTACTACCGCTGAATCTTGATGGGACCCCACTTTGCAAAGTGGATGCACCATAGATCAGGAGGTTAGGGGCTT8150.11639348710602948No Hit
CAGTAATAGACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCTTGAGGGAGAACTGGTTCTTGGACGTGTCTA7930.11325157702463973No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7840.11196625017316209No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG7730.11039529513246721No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA7640.1091099682809896No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7630.10896715418638096No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7590.10839589780794645No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC7550.10782464142951197No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7320.1045399172535136No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC7280.10396866087507908No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7250.1035402185912532No Hit
TCTTTAGCCTGGTATCAGCAAACACCAGGGAAAGCCCCTAACCTCCTGATCTATGGTGCATCCACTTTGCAAAG7240.1033974044966446No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC7220.10311177630742732No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT7200.10282614811821009No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7090.10125519307751521No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG7080.10111237898290656No Hit
ATAGGAGACAGAATCACCATCACTTGCCGGGCCAGTCAGGCCATTAGCAGTTCTTTAGCCTGGTATCAGCAAACA7040.10054112260447208No Hit
ATCTTGATGGGACCCCACTTTGCAAAGTGGATGCACCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTGTT7020.10025549441525483No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCCG150.0071276724144.7226470
CTCTTAT402.2009772E-1066.2928851
AATGGTA1851.8189894E-1258.67134570
TACCTCA600.006090359354.27099270
TAGTCTA200.00613170254.19071657
CGGCAAC200.006484654353.42945553
CTTATAC2900.048.0051881
TTATACA2950.047.174692
TATACAC3000.046.3884433
ATGCCGT3150.046.12953644
CGTATGC3000.045.71370741
AAAGGGG3400.044.94546568
TCGTATG3050.044.83840640
TGTTAGA8550.044.43239270
CCGTCTT3250.043.98011447
TGCTTGA3350.043.93821355
ATCTCGT3000.042.98024437
CTACATC2950.042.22501833
TCTACAT3050.041.8532932
CTTGAAA3550.041.72430457
Produced by FastQC (version 0.11.5)