FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955257
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGTACTGACTCAACCTCCCTCGGTGTCGGTGGCCCCAGGACAGACG96851.0138632849589169No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG50260.5261411327004146No Hit
GCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTCCGCCGAACACCTGACGATCATCACTACTATCCCACAC48550.5082401908596326No Hit
CTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTGAGGGTGGCCGTG40510.42407435904683244No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG38340.40135795916700956No Hit
GGATAGTAGTGATGATCGTCAGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTGGGTCAGCCCAAGGCTGCCCC34070.35665794650026117No Hit
GGCCTGGACCGTTCTCCTCCTCGGTCTCCTCTCTCACTGCACAGAGTCTGTGACCTCCTATGTACTGACTCAAC33840.3542502174807408No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTC31830.33320875952754075No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG31430.3290214047109835No Hit
GTCTGTGACCTCCTATGTACTGACTCAACCTCCCTCGGTGTCGGTGGCCCCAGGACAGACGGCCTCCGTTACATG29810.3120626177039268No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT28220.29541788230811183No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25330.26516424375848596No Hit
CCCCAGGACAGACGGCCTCCGTTACATGTGGGGGAAATAAGATTGGGACT24860.26024410184903124No Hit
GAGTCTGTGACCTCCTATGTACTGACTCAACCTCCCTCGGTGTCGGTGGCCCCAGGACAGACGGCCTCCGTTACA24810.25972068249696156No Hit
GGCCAGGCCCCTGTGCTGGTCGTCCAAGATGATAAAGACCGGCCCTCAGG24500.2564754825141297No Hit
CCCCTGTGCTGGTCGTCCAAGATGATAAAGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTC23680.2478914051401874No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGTCTCCTCTCTCACTGCACAGAGTCTGTGACCTCCTATGTACTGAC23140.24223847613783514No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22240.23281692780058144No Hit
GCACAGGGGCCTGGCCTGGCCTCTGTTGGTACCAATGTACACTTTTAGTCCCAATCTTATTTCCCCCACATGTAA22230.23271224393016748No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22140.2317700890964421No Hit
CCGTTACATGTGGGGGAAATAAGATTGGGACTAAAAGTGTACATTGGTACCAACAGAGGCCAGGCCAGGCCC21860.22883894072485206No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21470.22475626977870874No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20640.21606750853435255No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20640.21606750853435255No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20530.2149159859597993No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20500.21460193434855748No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20360.21313636016276247No Hit
CATCACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTGAGGG19300.20203986989888587No Hit
GTAGTGATGATCGTCAGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGG19200.20099303119474654No Hit
GTACCAACAGAGGCCAGGCCAGGCCCCTGTGCTGGTCGTCCAAGATGATA18500.1936651602657714No Hit
CTATTACTGTCAGGTGTGGGATAGTAGTGATGATCGTCAGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTGGG18480.19345579252494355No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGTCTCCTCTCTCACTGCACAGAGTCTGTGA18300.19157148285749281No Hit
GTACGGGAGTCGGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG18260.19115274737583707No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC18200.1905246441533535No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGC18180.19031527641252563No Hit
GCACAGAGTCTGTGACCTCCTATGTACTGACTCAACCTCCCTCGGTGTCGGTGGCCCCAGGACAGACGGCCTCCG17990.18832628287466094No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17820.18654665707762413No Hit
GGCCAGGCCAGGCCCCTGTGCTGGTCGTCCAAGATGATAAAGACCGGCCCTCAGGGATCCCTGAGCGATTCTC17730.18560450224389877No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG17700.18529045063265698No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC17700.18529045063265698No Hit
GAGTACGGGAGTCGGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG17670.18497639902141516No Hit
GTATCAACGCAGAGTACGGGAGTCGGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGT17660.18487171515100126No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17470.18288272161313657No Hit
CCTCTCTCACTGCACAGAGTCTGTGACCTCCTATGTACTGACTCAACCTC17280.18089372807527188No Hit
GACCCAGGACGGTCAACTTGGTCCCTCCGCCGAACACCTGACGATCATCA17180.17984688937113258No Hit
GTGCTGGTCGTCCAAGATGATAAAGACCGGCCCTCAGGGATCCCTGAGCG16950.17743916035161217No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTGAGGGT16830.176182953906645No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTCCGCCGAACACCTGACGATCA16690.17471737972084997No Hit
GTACTGACTCAACCTCCCTCGGTGTCGGTGGCCCCAGGACAGACGGCCTCCGTTACATGTGGGGGAAATAAGAT16330.1709487603859485No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTGAGGGTGG16100.1685410313664281No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT15890.16634267008773554No Hit
GTATTATTGTGCGAGGTCCTACGGTTATCAGGCCTCTGACTACTGGGGCC15310.1602710056037276No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15310.1602710056037276No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15210.1592241668995883No Hit
CTCACTGCACAGAGTCTGTGACCTCCTATGTACTGACTCAACCTCCCTCGGTGTCGGTGGCCCCAGGACAGACGG15150.1585960636771047No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAAC15120.1582820120658629No Hit
CTGTTGGTACCAATGTACACTTTTAGTCCCAATCTTATTTCCCCCACATGTAACGGAGGCCGTCTGTCCTGGGGC15070.15775859271379325No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTTCTCGATGGGGGCTGGGAGGGCTTTG14710.15398997337889175No Hit
GGCCTGGCCTGGCCTCTGTTGGTACCAATGTACACTTTTAGTCCCAATCTTATTTCCCCCACATGTAACGGAGGC14550.15231503145226888No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGTCTCCTCTCTCACTGCACAGAGTCTGTGACCTC14260.1492791992102649No Hit
GATAAAGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTC14230.1489651475990231No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13990.14645273470908876No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13870.1451965282641216No Hit
ACCCAGGACGGTCAACTTGGTCCCTCCGCCGAACACCTGACGATCATCAC13640.1427887992446012No Hit
GTTCTCCTCCTCGGTCTCCTCTCTCACTGCACAGAGTCTGTGACCTCCTATGTACTGACTCAACCTCCCTCGGTG13530.14163727667004794No Hit
CCCTCACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGT13510.14142790892922008No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAG13320.13943891539135542No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13260.1388108121688718No Hit
GGACAGACGGCCTCCGTTACATGTGGGGGAAATAAGATTGGGACTAAAAG13230.13849676055763005No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCT13210.1382873928168022No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13170.13786865733514647No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12890.1349375089635564No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12860.1346234573523146No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12840.13441408961148676No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT12780.13378598638900316No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12570.13158762511031064No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG12520.131064205758241No Hit
GTCCTACGGTTATCAGGCCTCTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAACCTCCACCAAGGG12510.13095952188782706No Hit
CTTTTAGTCCCAATCTTATTTCCCCCACATGTAACGGAGGCCGTCTGTCCTGGGGCCACCGACACCGAGGGAGGT12430.13012205092451562No Hit
GAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCTTTATCATCTTGGACGACCAGCACAGGGGC12350.12928457996120415No Hit
GACTCAACCTCCCTCGGTGTCGGTGGCCCCAGGACAGACGGCCTCCGTTACATGTGGGGGAAATAAGATTGGGA12250.12823774125706486No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12130.12698153481209767No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12130.12698153481209767No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTG11980.12541127675588873No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11970.1253065928854748No Hit
GCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCT11760.12310823160678226No Hit
GTCTTTATCATCTTGGACGACCAGCACAGGGGCCTGGCCTGGCCTCTGTT11750.12300354773636833No Hit
CAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGTGACACGGCCACC11740.12289886386595439No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11730.12279417999554046No Hit
GTGTGGGATAGTAGTGATGATCGTCAGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTGGGTCAGCCCAAGG11720.12268949612512653No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11720.12268949612512653No Hit
TGGTGAGGGTGGCCGTGTCACCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCTTTATCA11510.12049113484643399No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11480.1201770832351922No Hit
GGGATCCCTGAGGGCCGGTCTTTATCATCTTGGACGACCAGCACAGGGGCCTGGCCTGGCCTCTGTTGGTACCAA11430.11965366388312255No Hit
GACTATTACTGTCAGGTGTGGGATAGTAGTGATGATCGTCAGGTGTTCGGCGGAGGGACCAAGTTGACCGTCC11150.1167225155115325No Hit
CCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGGTGTGGGATAGTAGTGATGATCGTCAGG11140.11661783164111855No Hit
GACTAAAAGTGTACATTGGTACCAACAGAGGCCAGGCCAGGCCCCTGTGC11100.11619909615946285No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10820.11326794778787279No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGTGACACGG10780.11284921230621707No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCT10700.11201174134290563No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10670.11169768973166383No Hit
GTACATAGGAGGTCACAGACTCTGTGCAGTGAGAGAGGAGACCGAGGAGGAGAACGGTCCAGGCCATGGCTGAGG10600.11096490263876632No Hit
CTGCACAGAGTCTGTGACCTCCTATGTACTGACTCAACCTCCCTCGGTGTCGGTGGCCCCAGGACAGACGGCCTC10530.11023211554586881No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10520.11012743167545487No Hit
ACTTGGTCCCTCCGCCGAACACCTGACGATCATCACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCC10440.10928996071214343No Hit
GACCAGCACAGGGGCCTGGCCTGGCCTCTGTTGGTACCAATGTACACTTTTAGTCCCAATCTTATTTCCCCCACA10270.10751033491510661No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC10220.10698691556303697No Hit
GTGTACATTGGTACCAACAGAGGCCAGGCCAGGCCCCTGTGCTGGTCGTC10150.10625412847013946No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10100.1057307091180698No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACT10000.10468387041393049No Hit
GGGTGGCCGTGTCACCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCTTTATCATCT9980.10447450267310264No Hit
ACAGAGGCCAGGCCAGGCCCCTGTGCTGGTCGTCCAAGATGATAAAGACCGGCCCTCAGGGATCCCTGAGCGAT9840.1030089284873076No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC9780.10238082526482402No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTCCGCCGAACAC9750.10206677365358222No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG9660.10112461881985686No Hit
CCCTCGGTGTCGGTGGCCCCAGGACAGACGGCCTCCGTTACATGTGGGGGAAATAAGATTGGGACTAAAAGTGTA9590.10039183172695933No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)