FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955257
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGTACTGACTCAACCTCCCTCGGTGTCGGTGGCCCCAGGACAGACG83520.8743196856971475No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT46360.4853144232389818No Hit
GCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTCCGCCGAACACCTGACGATCATCACTACTATCCCACAC43910.45966687498756875No Hit
CTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC37060.3879584237540264No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36650.38366638506705525No Hit
GGCCTGGACCGTTCTCCTCCTCGGTCTCCTCTCTCACTGCACAGAGTCTGTGACCTCCTATGTACTGACTCAAC32570.3409553659381716No Hit
GGATAGTAGTGATGATCGTCAGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTGGGTCAGCCCAAGGCTGCCCC32070.33572117241747507No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30370.3179249144471069No Hit
GTCTGTGACCTCCTATGTACTGACTCAACCTCCCTCGGTGTCGGTGGCCC30010.3141562951122054No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTC29500.30881741772109494No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27600.28892748234244814No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25530.26725792116676456No Hit
GAGTCTGTGACCTCCTATGTACTGACTCAACCTCCCTCGGTGTCGGTGGC25360.2654782953697277No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGTCTCCTCTCTCACTGCACAGAGTCTGT24570.2572082696070272No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24560.2571035857366133No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA23570.2467398825656342No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA23080.2416103729153516No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGTCTCCTCTCTCACTGCACAGAGTCTGTGACCTCCTATGTACTGACT22860.2393073277662451No Hit
CCCCAGGACAGACGGCCTCCGTTACATGTGGGGGAAATAAGATTGGGACTAAAAGTGTACATTGGTACCAACAGA22730.23794643745086402No Hit
GGCCAGGCCCCTGTGCTGGTCGTCCAAGATGATAAAGACCGGCCCTCAGG22690.23752770196920828No Hit
CCCCTGTGCTGGTCGTCCAAGATGATAAAGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTG21540.22548905687160628No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21060.22046423109173763No Hit
GCACAGGGGCCTGGCCTGGCCTCTGTTGGTACCAATGTACACTTTTAGTCCCAATCTTATTTCCCCCACATGTAA20920.2189986569059426No Hit
CCGTTACATGTGGGGGAAATAAGATTGGGACTAAAAGTGTACATTGGTACCAACAGAGGCCAGGCCAGGCCC20540.21502066983021323No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19800.20727406341958238No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19240.20141176667640226No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG19160.20057429571309085No Hit
CCTCTCTCACTGCACAGAGTCTGTGACCTCCTATGTACTGACTCAACCTCCCTCGGTGTCGGTGGCCCCAGGACA18450.19314174091370176No Hit
GTATCAACGCAGAGTACGGGAGTCGGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT18280.19136211511666495No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA18160.19010590867169777No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG18140.1898965409308699No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG17620.18445297966934554No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17600.18424361192851765No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG17600.18424361192851765No Hit
CTATTACTGTCAGGTGTGGGATAGTAGTGATGATCGTCAGGTGTTCGGCG17590.18413892805810372No Hit
CATCACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTGAGGGTG17550.183720192576448No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT17460.18277803774272264No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCT17300.18110309581609976No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTGAGGGTGGCCGTGTCACCAGAGTTGGAGCCA16910.17702042486995648No Hit
GAGTACGGGAGTCGGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA16880.17670637325871466No Hit
GTACGGGAGTCGGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGT16710.17492674746167786No Hit
GGCCAGGCCAGGCCCCTGTGCTGGTCGTCCAAGATGATAAAGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTG16380.17147217973801815No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTG16320.17084407651553457No Hit
GCACAGAGTCTGTGACCTCCTATGTACTGACTCAACCTCCCTCGGTGTCG16110.16864571523684202No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGTCTCCTCTCTCACTGCACAGAGTCTGTGACCTC16070.1682269797551863No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG16050.16801761201435844No Hit
GTAGTGATGATCGTCAGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTG15960.16707545718063305No Hit
GACCCAGGACGGTCAACTTGGTCCCTCCGCCGAACACCTGACGATCATCA15890.16634267008773554No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15740.1647724120315266No Hit
GTGCTGGTCGTCCAAGATGATAAAGACCGGCCCTCAGGGATCCCTGAGCG15700.16435367654987088No Hit
GTACTGACTCAACCTCCCTCGGTGTCGGTGGCCCCAGGACAGACGGCCTCCGTTACATGTGGGGGAAATAAGATT15680.16414430880904302No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTCCGCCGAACACCTGACGATCAT15620.16351620558655944No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC15010.15713048949130967No Hit
GTATTATTGTGCGAGGTCCTACGGTTATCAGGCCTCTGACTACTGGGGCC14840.15535086369427284No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTT14320.14990730243274847No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT14220.14886046372860917No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG14190.14854641211736735No Hit
GTTCTCCTCCTCGGTCTCCTCTCTCACTGCACAGAGTCTGTGACCTCCTA14180.14844172824695342No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTGAGGGTGGCCGTGTCACCAGAGTTGGAGCCAGA14160.14823236050612557No Hit
GGCCTGGCCTGGCCTCTGTTGGTACCAATGTACACTTTTAGTCCCAATCTTATTTCCCCCACATGTAACGGAGGC14130.14791830889488378No Hit
CTGTTGGTACCAATGTACACTTTTAGTCCCAATCTTATTTCCCCCACATGTAACGGAGGCCGTCTGTCCTGGGGC14100.147604257283642No Hit
ACCCAGGACGGTCAACTTGGTCCCTCCGCCGAACACCTGACGATCATCACTACTATCCCACACCTGACAGTAATA13940.1459293153570191No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC13770.14414968955998228No Hit
GATAAAGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTC13770.14414968955998228No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTCCGCCGAACACCTGAC13680.1432075347262569No Hit
CTCACTGCACAGAGTCTGTGACCTCCTATGTACTGACTCAACCTCCCTCGGTGTCGGTGGCCCCAGGACAGACG13560.14195132828128976No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13380.140067018613839No Hit
GGACAGACGGCCTCCGTTACATGTGGGGGAAATAAGATTGGGACTAAAAGTGTACATTGGTACCAACAGAGGCCA13340.13964828313218328No Hit
GTACCAACAGAGGCCAGGCCAGGCCCCTGTGCTGGTCGTCCAAGATGATAAAGACCGGCCCTCAGGGATCCCTGA13220.13839207668721612No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC13180.13797334120556037No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13180.13797334120556037No Hit
CCCTCACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGGTGTGGGATAGTAGTGAT13030.13640308314935143No Hit
GACTCAACCTCCCTCGGTGTCGGTGGCCCCAGGACAGACGGCCTCCGTTACATGTGGGGGAAATAAGATTGG12520.131064205758241No Hit
GAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCTTTATCATCTTGGACGACCAGCACAGGGGC12450.13033141866534345No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12340.12917989609079022No Hit
GGGTGGCCGTGTCACCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCTTTATCATCTTG12220.12792368964582307No Hit
CTTTTAGTCCCAATCTTATTTCCCCCACATGTAACGGAGGCCGTCTGTCC12190.12760963803458128No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12060.12624874771920017No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTAT12050.12614406384878624No Hit
GTGTGGGATAGTAGTGATGATCGTCAGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTGGGTCAGCCCAAG12010.12572532836713052No Hit
GTCTTTATCATCTTGGACGACCAGCACAGGGGCCTGGCCTGGCCTCTGTT11820.12373633482926584No Hit
GTCCTACGGTTATCAGGCCTCTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAACCTCCACCAAGG11730.12279417999554046No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGG11610.1215379735505733No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11490.12028176710560613No Hit
GGGATCCCTGAGGGCCGGTCTTTATCATCTTGGACGACCAGCACAGGGGC11480.1201770832351922No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11410.11944429614229468No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11310.11839745743815538No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTG11300.11829277356774144No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTCCGCCGAACAC11280.1180834058269136No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAG11090.11609441228904892No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA11080.11598972841863497No Hit
CTGCACAGAGTCTGTGACCTCCTATGTACTGACTCAACCTCCCTCGGTGTCGGTGGCCCCAGGACAGACGGCCTC11020.11536162519615141No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTT10860.11368668326952852No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTC10840.11347731552870065No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGG10800.11305858004704494No Hit
TGGTGAGGGTGGCCGTGTCACCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCTTTATCAT10740.11243047682456134No Hit
GCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCT10660.1115930058612499No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGA10580.11075553489793845No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAG10560.1105461671571106No Hit
CAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGTGACACGGCCACCCTCACCATCAGCAGGGTCGAAGCCG10510.11002274780504094No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAA10450.10939464458255736No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10320.10803375426717628No Hit
ACTTGGTCCCTCCGCCGAACACCTGACGATCATCACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCC10240.10719628330386481No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAA10060.10531197363641408No Hit
GGACCGTTCTCCTCCTCGGTCTCCTCTCTCACTGCACAGAGTCTGTGACCTCCTATGTACTGACTCAACCTCCCT10040.10510260589558622No Hit
GACTATTACTGTCAGGTGTGGGATAGTAGTGATGATCGTCAGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTG9980.10447450267310264No Hit
CCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGGTG9950.10416045106186084No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTCCGCC9830.10290424461689368No Hit
CAATAATACATGGCGCTGTCCGAGGGCTTCAGGCTGTTCCACTGCAGGTAGGCGGTGTCGATGGAGTTGTCGGCC9750.10206677365358222No Hit
GACCAGCACAGGGGCCTGGCCTGGCCTCTGTTGGTACCAATGTACACTTT9750.10206677365358222No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGTGACACGG9700.10154335430151258No Hit
GACTAAAAGTGTACATTGGTACCAACAGAGGCCAGGCCAGGCCCCTGTGCTGGTCGTCCAAGATGATAAAGACCG9680.10133398656068471No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9680.10133398656068471No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC9630.10081056720861506No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGA9570.10018246398613148No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC9560.10007778011571755No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCA200.008103601138.6445970
CCGTTGT200.008103601138.6445970
GAAATAA35800.073.1950570
GACATAT650.003172725263.98981570
TAGGCAA700.00425521359.4191170
CCGGGTT800.007217550751.99172270
CGGCAAA800.007217550751.99172270
CGGACGA200.00749918551.50294543
GCCGTGT26650.045.7813370
GTGGTAT1150.041.4644241
GGTAGAA26200.041.27587570
GTATCAA15350.041.049591
TGGTATC1200.039.701382
TTACTTT1901.336334E-436.4854270
TATCAAC17650.035.2829782
TAACGGC701.0383737E-634.21602636
TTAACGG701.0426556E-634.19802535
ATCAACG18600.033.6620833
AACGCAG19100.033.3170936
GACAGGT2250.033.3031771
Produced by FastQC (version 0.11.5)