FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences782210
Sequences flagged as poor quality0
Sequence length19-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG25980.33213587144117307No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGAGTGCTGCTGGTTGTATATGAGCTA22290.28496183889236903No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21160.2705155904424643No Hit
GTAATAAACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA20120.25721992815228645No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCC19790.25300111223328775No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG18550.2371485918103834No Hit
GATTATTACTGTAGCTCATATACAACCAGCAGCACTCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGG18130.23177918973165773No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGAGTGCTGCTGGTTGTATATGAGCTACAGTAATAA18040.2306286035719308No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC17150.21925058488129787No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT15330.19598317587348665No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT15250.19496043262039606No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14790.1890796589151251No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT14330.18319888520985414No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC14130.18064202707712762No Hit
GTAATAATCAGCCTCGTCCTCAGTCTGGAGCCCAGAGATGGTCAGGGAGG14080.180002812543946No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13540.17309929558558443No Hit
TCTCTGGGCTCCAGACTGAGGACGAGGCTGATTATTACTGTAGCTCATAT13490.17246008105240282No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13410.1714373377993122No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13100.16747420769358612No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12980.16594009281395022No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12970.16581224990731389No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG12970.16581224990731389No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12930.1653008782807686No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGCGCTTATTCCTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC12800.16363892049449635No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT12540.16031500492195191No Hit
GTCTGGAGCCCAGAGATGGTCAGGGAGGCCGTATTGCCAGACTTGGAGCC12220.1562240319095895No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11880.15187737308395444No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC11780.1505989440175912No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11700.1495762007645006No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACAT11580.14804208588486467No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG11570.14791424297822833No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11560.14778640007159202No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGCGCTTATTCCTATGTCTCCTGGTACCAACAACA11560.14778640007159202No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11300.14446248449904756No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11060.14139425473977577No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG11020.14088288311323047No Hit
GTATGTGGTAACACCACTAAAACTAATACTTGAGACCCACTCCAGCCCCTTTCCTGGAGCCTGGCGGACCCAAT10890.13922092532695823No Hit
ACCACATACTACGCAGACTCCGTGAAGGGCCGGTTCTCCATCTCCAGAGACAATTCCAAGAACACGCTGTATC10870.13896523951368558No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10640.1360248526610501No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10560.13500210940795948No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10450.13359583743495992No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10240.1309111363955971No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10120.12937702151596117No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA10050.1284821211695069No Hit
GTTTATTACTGTGCGAAAGATTATATGAAGTATTACTATGAGACGATTGG9900.12656447756996203No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9900.12656447756996203No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9830.12566957722350774No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9760.12477467687705347No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAG9270.11851037445187354No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGAGTGC9230.11799900282532824No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGG9150.11697625957223763No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9070.11595351631914702No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT9030.11544214469260174No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9030.11544214469260174No Hit
GCTCCAGACTGAGGACGAGGCTGATTATTACTGTAGCTCATATACAACCA8900.1137801869063295No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACATAGGAATAAGCGCCAATGTCACTGCTGGTTCCAG8870.11339665818642052No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8850.11314097237314787No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC8850.11314097237314787No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC8720.11147901458687566No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGAG8720.11147901458687566No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG8690.11109548586696667No Hit
GTATTACTATGAGACGATTGGTTACCAGTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATC8480.10841078482760383No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8480.10841078482760383No Hit
CTCCTGTGAAGCCTCCGGATTCACCTTTGACAGTTATGGCATGAATTGGGTCCGCCAGGCTCCAGGAAAGGGG8430.1077715702944222No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8280.10585392669487732No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8280.10585392669487732No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT8280.10585392669487732No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8260.10559824088160467No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGAGTGCTGCTGG8140.10406412600196878No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTG8120.10380844018869613No Hit
TAGTAATACTTCATATAATCTTTCGCACAGTAATAAACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGA8090.10342491146878716No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7970.10189079658915125No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG7930.10137942496260596No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC7860.10048452461615168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1950.074.22578470
TACTTAC150.002416335368.5559339
ACGGTAA252.6188308E-454.97559742
TCGTATG1500.054.8729940
ATCTCGT1400.053.7961637
CGTATGC1550.053.14735841
TCTCGTA1450.051.97451438
CTGTTTA1208.3032137E-751.69295570
CCGTCTT1700.050.72858447
AGGCAAT1700.050.24071532
TCTTCGG1301.4418365E-647.71657670
GTATGCC1850.046.43209542
CGAGACA1850.046.04021523
AACGGTA306.4709195E-445.7657841
GCCGTCT1900.045.35349746
ATGCCGT1900.045.294944
CACGAGA1950.043.6763821
GGTATCA5900.043.3090131
ATCTCCG2000.042.5790310
TCTCCGA2000.042.57630511
Produced by FastQC (version 0.11.5)