FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004685268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences900336
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTATGTTGCATGCAATAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATCTTCAGTGTGAAATCAG41080.4562741021129889No Hit
GTAATACACAGCCGTGTCCTCGGGTCTCAGGCTATTCATTTGCAAATACA40580.45072061985747547No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT35420.39340868298057613No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34880.3874109221446216No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTGGTCAAAGCCTCGTA31880.3540900286115406No Hit
ACATACATACTGGCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA25100.27878480922677756No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT24060.26723356613530946No Hit
CATACATACTGGCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA23440.26034724813847276No Hit
GTGTATTACTGTGCGGGATGGGGATCTGATAATATTTGGGGCCAGGGATCCCTGGTCACCGTCTCCTCAGCATCC22640.2514616765296511No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21770.24179861740505765No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20860.2316912797000231No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20290.22536030992873773No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA20240.22480496170318637No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20150.22380533489719392No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG19720.21902934015745235No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC19550.21714115619057775No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19450.21603045973947504No Hit
GTTTCAAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTC19340.21480869364326208No Hit
CAGTAATACACAGCCGTGTCCTCGGGTCTCAGGCTATTCATTTGCAAATACAGTGAGTTCTTGGAGTTGTCCC19130.2124762310959464No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18610.20670060955021238No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18600.2065895399051021No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17660.19614899326473673No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC17570.19514936645874428No Hit
GTGATGGAAGCACCACCTTCAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTACAAGGTT17360.1928169039114286No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16600.1843756108830481No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC16510.1833759840770557No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15990.17760036253132164No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT15760.17504576069378544No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT15440.17149153205025677No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTACAAGGTTTCAAACCGGGAC14990.16649339802029464No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTGAGGCCAGTA14730.16360558724742763No Hit
GTCCAAGGGTGACGGGCAGGGAGAGAGGAGACTGAGTCAGCACAACATCGCCACTGCATGCTGGGACCCAGAGCA14500.1610509854098914No Hit
GTCTGGGACCCCAGAGTCCCGGTTTGAAACCTTGTAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA14140.1570524781859217No Hit
GGCCAAAAGTGTGAGGCCAGTATGTATGTTGCATGCAATAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGA14060.15616392102503954No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG13860.15394252812283415No Hit
CTCCTGTGCAGCCTCTGAATTCACCTTCAGTACCTATTGGATGAACTGGGTCCGTCAGGCTCCAGGGAAGGGG13860.15394252812283415No Hit
CCTCTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTGGTCAAAGCCTCGTAGACAG13770.1529429013168417No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13710.15227648344618008No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGCATGCAGTGGCGATGTTGTGCTGA13610.1511657869950774No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC13440.1492776030282028No Hit
GTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGAATTCACCTTCAGTAC13350.1482779762222104No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13150.14605658332000498No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAAGATCAGCAGGGTGGAGGCTGAGGA12990.14427946899824065No Hit
GCTAATGCTCTGGGTCCCAGCATGCAGTGGCGATGTTGTGCTGACTCAGTCTCCTCTCTCCCTGCCCGTCACCCT12860.14283556361180716No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12840.1426134243215866No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGCATGCAGTGGCGATGTTGTGCTGACTCAGTCTCCTCTCTCC12740.1415027278704839No Hit
GTGTGTGGACTCTGTGAAGGGCCGATTCACCGTCTCCAGGGACAACTCCAAGAACTCACTGTATTTGCAAATGAA12570.1396145439036093No Hit
GTGTGGGGTACAATTGGTGCAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC12550.13939240461338878No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12260.13617138490519096No Hit
GTATTACTGTGCGGGATGGGGATCTGATAATATTTGGGGCCAGGGATCCC12250.13606031526008067No Hit
GATTTCACACTGAAGATCAGCAGGGTGGAGGCTGAGGATGTTGGGGTTTATTATTGCATGCAACATACATAC12140.13483854916386773No Hit
CTCCAGCCCCTTCCCTGGAGCCTGACGGACCCAGTTCATCCAATAGGTAC12110.1345053402285369No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12100.13439427058342662No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11950.13272822590677258No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG11900.13217287768122124No Hit
CTCGTAGACAGTGATGGAAGCACCACCTTCAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATT11860.13172859910078016No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGG11730.13028469371434664No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA11700.12995148477901583No Hit
GTTTGAAACCTTGTAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCT11640.12928506690835423No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11540.12817437045725152No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11360.12617511684526664No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11280.12528655968438449No Hit
GGTCAAAGCCTCGTAGACAGTGATGGAAGCACCACCTTCAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGG11230.12473121145883315No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG11170.12406479358817153No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11160.12395372394306124No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA11080.1230651667821791No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11010.12228767926640721No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10890.12095484352508397No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10800.11995521671909154No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10730.11917772920331965No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTGGTCAAAGCCTCGTAGACAGTGA10320.1146238737537986No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA10240.11373531659291643No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG10220.11351317730269588No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT10180.1130688987222548No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10120.11240248085159318No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10030.11140285404560077No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGCATGCAGTGGCGATGTTGTGCTGACTCAGTCTCCTC10020.11129178440049048No Hit
GGGCTGGAGTGGGTGGCCAACATAAAGCCAGATGGAAGTGGGGAAAGGTG9990.11095857546515968No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9980.11084750582004939No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG9910.11007001830427753No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9900.10995894865916725No Hit
GAATTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGCCCAGTGTGGGGTACAATTGGTGCAG9740.10818183433740293No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9730.10807076469229264No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9650.1071822075314105No Hit
CTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTT9550.1060715110803078No Hit
GGGTCCCAGCATGCAGTGGCGATGTTGTGCTGACTCAGTCTCCTCTCTCCCTGCCCGTCACCCTTGGACAGCCGG9540.10596044143519753No Hit
GTCTACGAGGCTTTGACCAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGAGGAGA9490.10540509320964618No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9430.10473867533898455No Hit
GTCCACACACCTTTCCCCACTTCCATCTGGCTTTATGTTGGCCACCCACT9380.1041833271134332No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG9370.10407225746832294No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9330.10362797888788186No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC9330.10362797888788186No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT9150.101628725275897No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG9130.10140658598567645No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG9120.10129551634056619No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAG9050.1005180288247943No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT9050.1005180288247943No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)