FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences900336
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTATGTTGCATGCAATAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATCTTCAGTGTGAAATCAGT40660.45160917701835757No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34880.3874109221446216No Hit
GTAATACACAGCCGTGTCCTCGGGTCTCAGGCTATTCATTTGCAAATACAGTGAGTTCTTGGAGTTGTCCCTGGA33440.3714168932487427No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA32520.3611984858985979No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31400.3487586856462476No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTGGTCAAAGCCTCGTA28720.3189920207566953No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT22940.25479376588295927No Hit
ACATACATACTGGCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT22880.25412734801229764No Hit
GTGTATTACTGTGCGGGATGGGGATCTGATAATATTTGGGGCCAGGGATCCCTGGTCACCGTCTCCTCAGCATCC22250.24712996037035062No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC21200.23546764763377226No Hit
CATACATACTGGCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT20540.22813705105649446No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20340.22591565815428907No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG19580.21747436512590856No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC19110.21225409180572588No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19080.21192088287039507No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18790.2086998631621972No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCT18160.20170247552025022No Hit
GTTTCAAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTC17750.19714862007072917No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC17350.19270583426631835No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA17180.19081765029944378No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16870.18737449130102538No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC16230.18026603401396812No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA15970.1773782232411011No Hit
GTGATGGAAGCACCACCTTCAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTACAAGGTTT15590.17315757672691084No Hit
CAGTAATACACAGCCGTGTCCTCGGGTCTCAGGCTATTCATTTGCAAATACAGTGAGTTCTTGGAGTTGTCCCTG15440.17149153205025677No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15090.16760409447139735No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT14800.16438307476319952No Hit
CTCCTGTGCAGCCTCTGAATTCACCTTCAGTACCTATTGGATGAACTGGGTCCGTCAGGCTCCAGGGAAGGG14190.15760782641147306No Hit
GTCTGGGACCCCAGAGTCCCGGTTTGAAACCTTGTAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA13970.1551642942190471No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13970.1551642942190471No Hit
GTCCAAGGGTGACGGGCAGGGAGAGAGGAGACTGAGTCAGCACAACATCG13950.15494215492882657No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGCATGCA13600.15105471734996714No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA13500.1499440208988644No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGCATGCAGTGGCGATGTTGTGCT13200.14661193154555632No Hit
GTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGAATTCACCTTCAGTAC13190.14650086190044606No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC13140.14594551367489472No Hit
CCTCTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTGGTCAAAGCCTCGTAGACAGT13000.1443905386433509No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTACAAGGTTTCAAACCGGGACTCTGGGGTCCCAGACAGATTCAGCG12870.14294663325691742No Hit
GGCCAAAAGTGTGAGGCCAGTATGTATGTTGCATGCAATAATAAACCCCAACATCCTCAGCCTCCACCCTGCT12620.14016989212916067No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC12580.1397256135487196No Hit
CTCCAGCCCCTTCCCTGGAGCCTGACGGACCCAGTTCATCCAATAGGTACTGAAGGTGAATTCAGAGGCTGCACA12490.13872598674272715No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12360.13728208135629366No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG12200.13550496703452933No Hit
GTGTGTGGACTCTGTGAAGGGCCGATTCACCGTCTCCAGGGACAACTCCA12150.134949618808978No Hit
GTGTGGGGTACAATTGGTGCAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC12110.1345053402285369No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12110.1345053402285369No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11990.13317250448721366No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGG11990.13317250448721366No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11910.1322839473263315No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11900.13217287768122124No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11900.13217287768122124No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA11880.1319507383910007No Hit
GTTTGAAACCTTGTAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCT11780.13084004193989798No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11770.13072897229478772No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG11570.1285075793925823No Hit
CTCGTAGACAGTGATGGAAGCACCACCTTCAATTGGTTTCAGCAGAGGCC11330.12584190790993585No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAAGATCAGCAGGGTGGAGGCTGAGGAT11290.12539762932949478No Hit
GCTAATGCTCTGGGTCCCAGCATGCAGTGGCGATGTTGTGCTGACTCAGT11290.12539762932949478No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGCATGCAGTGGCGATGTTGTGCTGACTCAGTCTCCTCTCTCC11040.12262088820173803No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG11040.12262088820173803No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG10820.12017735600931206No Hit
GTATTACTGTGCGGGATGGGGATCTGATAATATTTGGGGCCAGGGATCCCTGGTCACCGTCTCCTCAGCATCCCC10810.1200662863642018No Hit
GGGTGGAGGCTGAGGATGTTGGGGTTTATTATTGCATGCAACATACATACTGGCCTCACACTTTTGGCCAGGG10560.11728954523644505No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT10500.11662312736578345No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10460.11617884878534236No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10240.11373531659291643No Hit
GTGTGAAATCAGTGCCTGACCCACTGCCGCTGAATCTGTCTGGGACCCCA10210.11340210765758561No Hit
GATTTCACACTGAAGATCAGCAGGGTGGAGGCTGAGGATGTTGGGGTTTATTATTGCATGCAACATACATACTG10160.11284675943203427No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10120.11240248085159318No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG10040.11151392369071103No Hit
CTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACC10030.11140285404560077No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG9860.10951467007872617No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9800.10884825220806454No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA9740.10818183433740293No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG9610.10673792895096942No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9610.10673792895096942No Hit
GGGCTGGAGTGGGTGGCCAACATAAAGCCAGATGGAAGTGGGGAAAGGTG9590.10651578966074887No Hit
GGTCAAAGCCTCGTAGACAGTGATGGAAGCACCACCTTCAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAG9570.10629365037052835No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9500.10551616285475644No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTGGTCAAAGCCTCGTAGACAGTGATG9490.10540509320964618No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9480.10529402356453592No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9460.10507188427431537No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA9340.10373904853299212No Hit
GAATTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGCCCAGTGTGGGGTACAATTGGTGCAGT9320.1035169092427716No Hit
GTCTACGAGGCTTTGACCAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGAGGA9270.10296156101722025No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC9240.10262835208188943No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9220.10240621279166888No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATTA455.1417924E-4101.1955770
AGAGCAT6900.076.9966370
AATGGTA2450.061.9564770
CTCCCTT850.006409244653.57412370
CCTTCAA21900.051.98402470
TAGGGTC306.8677095E-445.2170820
GAGCAAA18300.043.96201370
GTGGTAT1500.042.970561
CGATTCA10500.041.9238870
GACAGGT2150.041.0245171
GGTATCA6600.040.6061481
GTATCAA15750.038.9858171
CGTCACA9050.038.57731670
CCAGTAT12700.037.0519270
GAGCCAT9650.036.17872670
TATTGTT2151.621807E-435.30077770
AAGCGGT7050.034.4495670
TGGTTCA601.5004636E-533.94299725
ACAGATC601.5061889E-533.924121
TTATGCC502.0693702E-433.882674
Produced by FastQC (version 0.11.5)