FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080717
Sequences flagged as poor quality0
Sequence length19-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATGAC53340.4935612190795555No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG49340.456548754206698No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36900.34143998845211093No Hit
CTCCTGCACTGGGAGCAACTCCAACATCGGGACAGGTTCTGGTGTACACTGGTACCAGCAACTTCCAGGAACACC33010.305445366363257No Hit
GGTGTACACTGGTACCAGCAACTTCCAGGAACACCCCCCAAACTCCTCAT32170.29767274873995686No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC30910.28601382230500677No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG30150.27898145397916385No Hit
GTCTCTGGTGGTTCCATCAACAATATTGGTTACTACTGGGGCTGGGTCCGCCAGCCCCCCGGGAAGGGCCTGGAG28350.26232584478637794No Hit
GTGTACACCAGAACCTGTCCCGATGTTGGAGTTGCTCCCAGTGCAGGAGA25230.23345612218554904No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT24970.2310503119688133No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22850.21143370558619878No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21720.2009776842596165No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21330.19736896893451294No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21090.19514822104214147No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20980.1941303782581379No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACCTCCACTGCTCAGGCGTCAGGCTCAGATAG20720.19172456804140214No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20300.18783825922975209No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20280.18765319690538781No Hit
GGACAGAGGGTCACCATCTCCTGCACTGGGAGCAACTCCAACATCGGGACAGGTTCTGGTGTACACTGGTACCAG20160.18654282295920208No Hit
ATGATAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCCGGCACCTCAGCCTCCC20010.18515485552646993No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19750.18274904530973418No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCGGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATTGGTG19580.18117601555263774No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19260.17821501836280915No Hit
GATTATTACTGCCAGTCTTCTGTCAATGACCTAGGTGGTCCTGTCATATTCGGCGGAGGGACCAAGCTGACCGTC19200.17765983138971628No Hit
GACCTAGGTGGTCCTGTCATATTCGGCGGAGGGACCAAGCTGACCGTCCT19110.17682705093007697No Hit
GTTCTGGTGTACACTGGTACCAGCAACTTCCAGGAACACCCCCCAAACTCCTCATCTATGATAACACCAATCGGC18620.17229302398315194No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAG18490.17109011887478406No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACCTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC18270.16905443330677689No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18200.16840671517150188No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCTTCTGTCAATGACCTAGGTGGTC18090.1673888723874983No Hit
CACCTGCACTGTCTCTGGTGGTTCCATCAACAATATTGGTTACTACTGGG17820.16489053100858042No Hit
GAATATGACAGGACCACCTAGGTCATTGACAGAAGACTGGCAGTAATAAT17650.16331750125148395No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17390.16091169103474823No Hit
GTGTTATCATAGATGAGGAGTTTGGGGGGTGTTCCTGGAAGTTGCTGGTA17390.16091169103474823No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16990.15721044454746247No Hit
CTACTGGGGCTGGGTCCGCCAGCCCCCCGGGAAGGGCCTGGAGTGGATTGGAAGTATCTCTCACAGTGGGACCAC16860.1560075394390946No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATGACAGGACCACCT16710.15461957200636245No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG16670.15424944735763388No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT16620.15378679154672314No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACCTCCACTGCTCAGGCGTCAGGCTCAGATAGC16600.15360172922235887No Hit
GTCATATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG16580.15341666689799457No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG16240.15027060738380169No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATGACAGGACCACCTA15950.1475872036805195No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACAATGTCCGTAGACACGACCAAGAATCAGTTCTTCCTGAAG15910.14721707903179093No Hit
GTGCAGGAGATGGTGACCCTCTGTCCTGGGGCCCCAGACACTGAGGGCGG15820.14638429857215163No Hit
ACCTAGGTCATTGACAGAAGACTGGCAGTAATAATCAGCCTCATCCTCAG15650.14481126881505518No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG15260.1412025534899516No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT15190.14055483535467658No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15030.13907433675976227No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGC15030.13907433675976227No Hit
GGATGAGGCTGATTATTACTGCCAGTCTTCTGTCAATGACCTAGGTGGTC14880.1376863693270301No Hit
CCCCCCAAACTCCTCATCTATGATAACACCAATCGGCCCTCAGGGGTCCC14710.13611333956993366No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCTTCTGTCAATGACCTAGGTGGTCCTGTCA14690.13592827724556936No Hit
CCCCAGGACAGAGGGTCACCATCTCCTGCACTGGGAGCAACTCCAACATC14450.1337075293531979No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14280.13213449959610146No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATGACAGGACCAC14160.13102412564991575No Hit
GTAGTAGGTGGTCCCACTGTGAGAGATACTTCCAATCCACTCCAGGCCCTTCCCGGGGGGCTGGCGGACCCAGCC14130.13074653216336932No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14070.13019134519027645No Hit
GTGTCTGGGGCCCCAGGACAGAGGGTCACCATCTCCTGCACTGGGAGCAACTCCAACATCGGGACAGGTTCTGG14000.12954362705500144No Hit
GCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCC13800.12769300381135856No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13760.12732287916263No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGG13680.12658262986517285No Hit
GTTTGGGGGGTGTTCCTGGAAGTTGCTGGTACCAGTGTACACCAGAACCTGTCCCGATGTTGGAGTTGCTCCCAG13600.1258423805677157No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCGGACTTGGAGCC13550.12537972475680498No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13540.12528719359462284No Hit
GCTTGGTCCCTCCGCCGAATATGACAGGACCACCTAGGTCATTGACAGAAGACTGGCAGTAATAATCAGCCTCA13440.12436188197280139No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13380.12380669499970852No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13350.12352910151316208No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG13350.12352910151316208No Hit
CCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGG13210.12223366524261207No Hit
CTCCAACATCGGGACAGGTTCTGGTGTACACTGGTACCAGCAACTTCCAGGAACACCCCCCAAACTCCTCATCTA13180.12195607175606565No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13150.12167847826951922No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12960.11992038618805848No Hit
GGTTCTGGTGTACACTGGTACCAGCAACTTCCAGGAACACCCCCCAAACTCCTCATCTATGATAACACCAATCGG12780.1182548252687799No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12640.11695938899822989No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12630.11686685783604775No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12530.11594154621422631No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAAACGGTGACCAGGGTGCCACGG12510.11575648388986201No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12390.11464610994367627No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC12100.11196270624039412No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12060.11159258159166552No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG11980.11085233229420838No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11970.11075980113202624No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACAATGTCCGTAGACACGACC11960.11066726996984408No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG11870.1098344895102048No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11860.10974195834802264No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11710.1083539909152905No Hit
GGCCTGGAGTGGATTGGAAGTATCTCTCACAGTGGGACCACCTACTACAA11700.10826145975310836No Hit
GTCAATGACCTAGGTGGTCCTGTCATATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCC11600.10733614813128692No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11410.10557805604982619No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGG11400.10548552488764404No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA11340.10493033791455118No Hit
TGACAGGACCACCTAGGTCATTGACAGAAGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGA11220.10381996396836544No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11030.1020618718869047No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11000.1017842784003583No Hit
GACCTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTG10920.10104402910290113No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG10890.10076643561635469No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCCGGCACCTCAG10880.10067390445417256No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG10880.10067390445417256No Hit
CACCAGAACCTGTCCCGATGTTGGAGTTGCTCCCAGTGCAGGAGATGGTGACCCTCTGTCCTGGGGCCCCAGACA10830.10021124864326182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1400.089.9599970
GACCGGA803.2926374E-452.47665870
TCGTATG1500.045.7099940
TCTCGTA1350.045.61186238
TCTCGCG8350.045.24933670
CGTATGC1550.044.27689441
CACGAGA1550.043.76671621
GCCGTCT1500.043.70453346
GGTATCA7500.043.416581
ACGAGAC1600.042.40096722
GTATGCC1650.041.64608442
GTGGTAT1800.041.4568021
GTATCAA20200.040.635881
ACTCTCA1050.001257314539.9822270
ATCTCGT1650.039.34982337
CCCACGA1750.038.76480519
CCGTCTT1700.038.602847
CGAGACA1850.038.5046623
ATGCCGT1750.037.37657544
TAGGCAA2304.3006366E-836.505570
Produced by FastQC (version 0.11.5)