FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004685273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080717
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT50760.46968817923656236No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATGACAGGACCACCTAGGTCATTGACAGAA41880.3875205072188186No Hit
GTCCTGGGCCCAGTCTGTACTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGACAGAGGGTCACCATCTCCT35370.32728272063824293No Hit
GGTGTACACTGGTACCAGCAACTTCCAGGAACACCCCCCAAACTCCTCATCTATGATAACACCAATCGGCCCTCA34620.3203428834745821No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33310.3082213012287213No Hit
CTCCTGCACTGGGAGCAACTCCAACATCGGGACAGGTTCTGGTGTACACTGGTACCAGCAACTTCCAGGAACACC30960.2864764781159175No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29250.2706536493827709No Hit
GTACTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGACAGAGGGTCAC28460.2633436875703815No Hit
GTCTCTGGTGGTTCCATCAACAATATTGGTTACTACTGGGGCTGGGTCCG27540.25483082064962426No Hit
AGTCTGTACTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGACAGAGG24460.22633122269752398No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC23870.22087188412877748No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23130.21402457812729883No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT22930.21217395488365595No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21200.19616606382614507No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21020.19450050290686646No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA20880.19320506663631645No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20360.18839344620284496No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACCTCCACTGCTCAGGCGTCAGGCTCAGATA20200.18691294760793065No Hit
ATGATAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCCGGCACCTCAGCCTCCCTG20010.18515485552646993No Hit
GGACAGAGGGTCACCATCTCCTGCACTGGGAGCAACTCCAACATCGGGACAGGTTCTGGTGTACACTGGTACCA19730.1825639829853699No Hit
GATTATTACTGCCAGTCTTCTGTCAATGACCTAGGTGGTCCTGTCATATTCGGCGGAGGGACCAAGCTGACCGTC19580.18117601555263774No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG18660.1726631486318805No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18190.16831418400931974No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTACTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGACAGAGGGTCAC18190.16831418400931974No Hit
GACCTAGGTGGTCCTGTCATATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCG18180.16822165284713758No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17840.1650755933329447No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG17770.1644278751976697No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCTTCTGTCAATGACCTAGGTGGTC17510.16202206498093394No Hit
GTTCTGGTGTACACTGGTACCAGCAACTTCCAGGAACACCCCCCAAACTCCTCATCTATGATAACACCAATCGGC17190.15906106779110535No Hit
CACCTGCACTGTCTCTGGTGGTTCCATCAACAATATTGGTTACTACTGGGGCTGGGTCCGCCAGCCCCCCGGGAA17190.15906106779110535No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA16650.15406438503326958No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG16620.15378679154672314No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16530.15295401108708387No Hit
GCACAGGGTCCTGGGCCCAGTCTGTACTGACGCAGCCGCCCTCAGTGTCT16340.15119591900562313No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG16300.15082579435689455No Hit
GTCATATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCC16170.14962288924852668No Hit
CTACTGGGGCTGGGTCCGCCAGCCCCCCGGGAAGGGCCTGGAGTGGATTGGAAGTATCTCTCACAGTGGGACCAC15980.14786479716706594No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACAATGTCCGTAGACAC15950.1475872036805195No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACCTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTG15940.14749467251833737No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC15850.14666189205869806No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT15680.14508886230160162No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15380.1423129274361373No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACCTCCACTGCTCAGGCGTCAGGCTCAGATAGCT15130.1399996483815837No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG14880.1376863693270301No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG14810.1370386511917551No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14320.13250462424483003No Hit
GGATGAGGCTGATTATTACTGCCAGTCTTCTGTCAATGACCTAGGTGGTCCTGTCATATTCGGCGGAGGGACCAA14130.13074653216336932No Hit
GAATATGACAGGACCACCTAGGTCATTGACAGAAGACTGGCAGTAATAAT13990.1294510958928193No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCTTCT13950.12908097124409074No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13920.1288033777575443No Hit
GTGTACACCAGAACCTGTCCCGATGTTGGAGTTGCTCCCAGTGCAGGAGA13840.12806312846008716No Hit
CCCCAGGACAGAGGGTCACCATCTCCTGCACTGGGAGCAACTCCAACATC13730.12704528567608356No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCGGACTTGGAGCCAGAGAATCGGTC13730.12704528567608356No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT13670.12649009870299072No Hit
CTCCAACATCGGGACAGGTTCTGGTGTACACTGGTACCAGCAACTTCCAG13580.1256573182433514No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13280.12288138337788708No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13220.12232619640479422No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGA13130.12149341594515493No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT13110.12130835362079065No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC13060.12084569780987992No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA12950.11982785502587634No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12820.11862494991750847No Hit
GTAGTAGGTGGTCCCACTGTGAGAGATACTTCCAATCCACTCCAGGCCCTTCCCGGGGGGCTGGCGGACCCAGCC12740.11788470062005132No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG12740.11788470062005132No Hit
CCCCCCAAACTCCTCATCTATGATAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCC12570.11631167086295488No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC12540.11603407737640845No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC12520.11584901505204415No Hit
GTGTCTGGGGCCCCAGGACAGAGGGTCACCATCTCCTGCACTGGGAGCAACTCCAACATCGGGACAGGTTCTGGT12180.11270295553785126No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAAACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGTGCCAGTAGA12180.11270295553785126No Hit
GGTTCTGGTGTACACTGGTACCAGCAACTTCCAGGAACACCCCCCAAACTCCTCATCTATGATAACACCAATCG12160.11251789321348696No Hit
CCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT12090.11187017507821195No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG12060.11159258159166552No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT11900.11011208299675124No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11860.10974195834802264No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACAATGTCCGTAGACACGACCAAGAATCAGTTCTTCCTGAAGTTGA11810.10927930253711193No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCCGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCA11660.10789133510437977No Hit
GTGCAGGAGATGGTGACCCTCTGTCCTGGGGCCCCAGACACTGAGGGCGGCTGCGTCAGTACAGACTGGGCCCAG11640.10770627278001549No Hit
GTGTTATCATAGATGAGGAGTTTGGGGGGTGTTCCTGGAAGTTGCTGGTACCAGTGTACACCAGAACCTGTCCC11630.10761374161783335No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAGGTCCCACAGAAGCTACAGCTGC11580.10715108580692262No Hit
GGGTGCCACGGCCCCAGAGATCGAAGTGCCAGTAGAGGAGTAGAGTCGCCTCATGTCGCGCACAAAAATACACA11570.10705855464474048No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAAGACCTTGGG11560.10696602348255833No Hit
GTCCTGGCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGTCTGGTAAAGCC11490.10631830534728333No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATGACAGGACCACCTA11370.1052079314010976No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATGACAG11370.1052079314010976No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATGACAGGACCAC11350.10502286907673332No Hit
GGGTCCTGGGCCCAGTCTGTACTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGACAGAGGGTCACCATCTC11150.10317224583309044No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAG11120.102894652346544No Hit
GGCCTGGAGTGGATTGGAAGTATCTCTCACAGTGGGACCACCTACTACAA11000.1017842784003583No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10930.10113656026508326No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT10910.10095149794071899No Hit
GTCTGTACTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGACAGAGGGTCACCATCTCCTGCACTGGGAGCAA10900.10085896677853685No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATGACAGGACCACCT10860.10048884212980826No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)