FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1634973
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTACCTCACCGGGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGT71710.43860051511554016No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57810.35358382065024924No Hit
GTATGGTACCTCACCGGGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT56490.3455102928305238No Hit
GTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC53300.3259992672661873No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA46880.2867325637793407No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA46160.28232882133221776No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGGCTCCTCAACTCCATGTAAG44360.27131946521441025No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43500.26605943951368005No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA39950.244346542725782No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT34160.20893311388016805No Hit
GTCTCCAGACACCCTGTCTTTGTCTCCAGGGGAAAGAGGCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCA32880.2011042384186161No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA32760.2003702813440956No Hit
GTGTATTACTGTCAGCAGTATGGTACCTCACCGGGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAA32560.19914701955322808No Hit
GTGTATTACTGTGCGGGTACTAGCATATTGTGGGCCTCGGGGTACTTCGA31360.19180744880802314No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC31330.191623959539393No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30020.1836115948092109No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC29990.18342810554058078No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29100.17798459057122043No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC27510.16825965933382386No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGTCAGTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAG27030.16532383103574189No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT26270.1606754362304454No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA25940.15865705427551405No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG25590.15651634614149593No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC25200.1541309856493043No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGTCAGTGGCCCTGCTGGATGCACCGTAGATGAGGAGCCTG25160.1538863332911308No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC25080.15339702857478382No Hit
GCTCATCACCATGGACTGGACCTGGAGGGTCCTCTTTGTGGTGGCAGCAGCTACAGGTGTCCAGTCCCAGGTGCA25020.15303005003752354No Hit
GTGTTAGCAGCAGTCAGTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTACGGTGCAT24880.1521737667839163No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24440.1494825908440078No Hit
CAGTAATACACGGCCGTGTCGTCAGATCTCAGGCTCCTCAACTCCATGTAAGCTGTGCTCGTAGATTCGTCCGCG23980.14666908872501258No Hit
TCCCTATATTTGGGACATCAAACTACGCACAGAAGTTCCAGGGCGGAGTCACGATTACCGCGGACGAATCTACGA23370.14293814026286675No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT23120.14140906302428236No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA22930.14024696432295825No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22920.14018580123341487No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA22840.13969649651706786No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG22380.13688299439807264No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA22330.13657717895035576No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG21910.134008329189534No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG21800.1333355352045569No Hit
CTCCAGACACCCTGTCTTTGTCTCCAGGGGAAAGAGGCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCA21770.13315204593592678No Hit
GTCTTTGTCTCCAGGGGAAAGAGGCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGTCAGTTAGCCTG21530.1316841317868858No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC21500.13150064251825566No Hit
GTATTACTGTGCGGGTACTAGCATATTGTGGGCCTCGGGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCA20590.12593480136980856No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20550.12569014901163506No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGTCAGTGGCCCTGCTGGATGCACCGTA20320.12428339795213744No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20170.1233659516089868No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20070.12275432071355308No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA20050.12263199453446633No Hit
GACCTGGAGGGTCCTCTTTGTGGTGGCAGCAGCTACAGGTGTCCAGTCCCAGGTGCAGCTGGTGCAGTCTGGG19660.1202466340422747No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC19510.1193291876991241No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATATTTGGG19250.11773894737099634No Hit
GTCAGAGTGTTAGCAGCAGTCAGTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTACG19040.11645452249058547No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGACACCCTGTCTTTGTCTCCA19020.11633219631149871No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC18520.11327404183432999No Hit
CAGTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTACGGTGCATCCAGCAGGGCCA18390.11247892167026612No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18380.11241775858072274No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG18310.11198961695391911No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18300.11192845386437573No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18140.11094984443168175No Hit
CATCTACGGTGCATCCAGCAGGGCCACTGACATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA17570.1074635483277094No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC17190.10513935092506113No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17160.10495586165643102No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC17070.10440539385054065No Hit
GAGTTGAGGAGCCTGAGATCTGACGACACGGCCGTGTATTACTGTGCGGG16890.1033044582387599No Hit
ATATAGGGATGATCCCTCCCATCCACTCAAGCCCTTGTCCAGGGGCCTGTCGCACCCAGCTAATAGCATAACTA16460.10067444538839479No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTACCTCA16450.10061328229885143No Hit
CCTCATCTACGGTGCATCCAGCAGGGCCACTGACATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT16430.10049095611976468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)