FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690542
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21020.3043985738738556No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT19150.277318396274231No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG15280.22127546188356392No Hit
GTCTCTGGTGGCTCCCTCAATAGTGGTAGTTACTACTGGACCTGGATCCGGCAGCCCGCCGGGAAGGGACTGGAG14470.20954554538319173No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA14250.2063596421361771No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT14120.20447706294475934No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13940.2018704148335655No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13550.19622267725931225No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12820.18565127103058177No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG12730.18434794697498486No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12570.18203092643170146No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12540.18159648507983583No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12140.17580393372162736No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12050.17450060966603045No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11800.17088026506715015No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC11560.16740473425222507No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA11130.16117774154215095No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT10940.15842627964700193No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC10470.15162003180110697No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT10270.14872375612200273No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC10220.14799968720222667No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10170.1472756182824506No Hit
TAATAATACCAGCCTCTCTCTGTAGGAAATCCTCTCGTACAATAATATACGGCCGTGTCTGCGGCGGTCGCAGAA10010.14495859773916722No Hit
GGCCAAAAGTGTACGAGAGACTACTGAAACTCTGTTGACAATAGTAAGTTGCAAAATCTCCAGGTTGCAGAC9390.13598014313394408No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8880.12859464015222824No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC8590.12439504041752711No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGGAGATTTTGCAACTTACTATTGTCAACAGAGTTTCAGTAG8590.12439504041752711No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG8520.12338134392984061No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT8510.1232365301458854No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGCAA8500.1230917163619302No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8440.12222283365819891No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC8410.12178839230633329No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8330.12062988203469158No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGCAATTATTTAAATTGGTATCAACAAAAA8320.12048506825073638No Hit
GGACTGGAGTGGATCGGGCGTATTTATACCGATGGGAGCACCAAGCAAAA8250.1194713717630499No Hit
TATTTATACCGATGGGAGCACCAAGCAAAATTCCTTCCTCCAGAGTCGAG8210.11889211662722905No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7880.11411326175670704No Hit
CTCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG7830.113389192836931No Hit
GTGTACGAGAGACTACTGAAACTCTGTTGACAATAGTAAGTTGCAAAATC7790.11280993770111014No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC7540.10918959310222984No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACA7450.10788626904663294No Hit
ATAATATACGGCCGTGTCTGCGGCGGTCGCAGAACTCAGCTTCAGGGAGAACTGGTTCTTGGATGTGTCTGCTGA7450.10788626904663294No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGAGAGACTACTGAAACTCTGT7450.10788626904663294No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTT7350.10643813120708082No Hit
CACCTGCACTGTCTCTGGTGGCTCCCTCAATAGTGGTAGTTACTACTGGA7330.10614850363917039No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7300.10571406228730475No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT7290.10556924850334953No Hit
GTCTGCAACCTGGAGATTTTGCAACTTACTATTGTCAACAGAGTTTCAGT7210.10441073823170785No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC7200.10426592444775264No Hit
CCCTGAGGAGACTGTGACCAGGATTCCCTGGCCCCAGTAGTCATAATAATACCAGCCTCTCTCTGTAGGAAATC7030.10180409012051403No Hit
GTATAAATACGCCCGATCCACTCCAGTCCCTTCCCGGCGGGCTGCCGGATCCAGGTCCAGTAGTAACTACCACTA7020.10165927633655882No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG6950.10064557984887233No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)