FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335484
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGAATAAAACAC61160.4579613084095355No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT61120.45766179153026165No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC60640.45406758897897687No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTC53830.4030748402826241No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC45930.3439202566260621No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT41580.31134779600504386No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40060.2999661545926421No Hit
GTTTTATTCAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA38460.28798547942169284No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37620.28169562495694445No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG36570.273833306876009No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33170.24837437213774183No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33010.24717630462064688No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32920.246502391642281No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC32730.24507968646573078No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32150.2407366917162617No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAG30820.2307777554804101No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT30150.2257608477525751No Hit
GTCTCCAGACTCCCTGGCTGTGTATCTGGGCGAGAGGGCCACCATCAACTGCAAGTGCAGCCAGAGTGTTTTAT28840.2159516699563604No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAACCTGCAGGCTGAAGA28120.21056036612943324No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27980.20951205705197515No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG27740.20771495577633275No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC27050.2025482896088609No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC27010.2022487727295872No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26050.19506036762701762No Hit
CTCCCTGGCTGTGTATCTGGGCGAGAGGGCCACCATCAACTGCAAGTGCAGCCAGAGTGTTTTATTCAGCTCCAA25950.1943115754288333No Hit
GAATAAAACACTCTGGCTGCACTTGCAGTTGATGGTGGCCCTCTCGCCCAGATACACAGCCAGGGAGTCTGGAGA25930.19416181698919643No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25680.1922898364937356No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGAA25670.1922149572739172No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25070.1877222040848112No Hit
GTGTATCTGGGCGAGAGGGCCACCATCAACTGCAAGTGCAGCCAGAGTGTTTTATTCAGCTCCAACAATAAGAA24090.18038404054260476No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTG22640.16952655366893202No Hit
GATTTCACTCTCACCATCAGCAACCTGCAGGCTGAAGATGTGGCAATTTATTACTGTCAGCAATTTTATGATAC22630.16945167444911358No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATT22610.16930191600947672No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21760.16293718232490992No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21340.15979225509253572No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAG21100.15799515381689336No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21070.15777051615743806No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT20900.1564975694205247No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20720.1551497434637929No Hit
GTAATATGTGGCTGTGTCCACAGGGTCCATGTTGGTCATTGTAAGGATCACCTGGTTTTTGGAAGTGTCCTTGG20530.15372703828724268No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAACCTGCAGGCTGAAGATGTGGCAATTTA20190.15118114481341596No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20120.15065699027468693No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA20070.15028259417559478No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA18880.14137196701720126No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18720.14017389950010634No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18540.13882607354337453No Hit
GTATCTGGGCGAGAGGGCCACCATCAACTGCAAGTGCAGCCAGAGTGTTTTATTCAGCTCCAACAATAAGAACTA18080.13538162943172663No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17950.134408199574087No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTATCTGGGCGAGAGGG17830.1335096489362658No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG17690.13246133985880773No Hit
CCCTGGCTGTGTATCTGGGCGAGAGGGCCACCATCAACTGCAAGTGCAGC17600.13178742688044184No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17450.13066423858316537No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG17200.12879225808770453No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTATCTGGGCGAGAGGGCCACCATCAAC16950.1269202775922437No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT16890.1264710022733331No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT16750.12542269319587507No Hit
ATTTATTACTGTCAGCAATTTTATGATACTCTCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA16480.12340095426077737No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT16480.12340095426077737No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAACCTGCAGGCTGAAGATGTGGCAATTTATTACTGTCAGCAAT16470.12332607504095894No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16430.1230265581616852No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16400.12280192050222989No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16350.12242752440313773No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16310.12212800752386402No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16210.12137921532567968No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16160.12100481922658751No Hit
GTAATAAATTGCCACATCTTCAGCCTGCAGGTTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA16000.11980675170949258No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15910.1191328387311267No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15840.11860868419239766No Hit
CAGTAATAAATTGCCACATCTTCAGCCTGCAGGTTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA15440.11561351539966035No Hit
GCATTAGTGGAGTGGGTGTGGGCTGGATCCGTCAGCCCCCAGGAAAGGCCCTAGAGTGGCTTGCAGTCATTTAT15410.11538887774020505No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT15340.114864723201476No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATC15180.1136666556843811No Hit
CACCATCAGCAACCTGCAGGCTGAAGATGTGGCAATTTATTACTGTCAGCAATTTTATGATACTCTCCTCACTT15160.11351689724474422No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15150.11344201802492579No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG14970.112094192068194No Hit
TCTTCAGCCTGCAGGTTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC14890.11149515830964653No Hit
GAGTATCATAAAATTGCTGACAGTAATAAATTGCCACATCTTCAGCCTGCAGGTTGCTGATGGTGAGAGTGAAA14880.1114202790898281No Hit
GGCTGAAGATGTGGCAATTTATTACTGTCAGCAATTTTATGATACTCTCCTCACTTTCGGCGGAGGGACCAAGG14720.11022221157273317No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14440.10812559341781705No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGA14330.1073019219998143No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14320.10722704277999585No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAACCTGCAGGCTGAAGATGTGGCAATTTATTACTGTCAGCAATT14280.10692752590072215No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGG14200.10632849214217467No Hit
ATGATACTCTCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCA14150.10595409604308251No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14150.10595409604308251No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14140.10587921682326407No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCT13920.10423187398725856No Hit
GAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGC13740.10288404803052677No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC13730.10280916881070833No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA13730.10280916881070833No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG13630.102060376612524No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGT13620.10198549739270557No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG13530.10131158441433967No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGGAGAGTATCATAAAATTGCTGACAGTAATAAATTGCCACATCTTCAGC13480.1009371883152475No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCTA250.00289890965.478769
GTGGTAT2800.052.2001571
TCGTATG900.045.69252440
AATGGTA4600.045.37295570
GGTATCA10450.044.8873751
TGTTAGA16100.044.44697670
TGGTATC3250.043.903552
CGTATGC1050.039.20203841
AAAACCG1301.0172283E-438.22630370
GCTCGGG2601.2551027E-1038.22630370
GTACGTA600.00200174336.37705669
GCGTAGA37750.035.80614570
GTATCAA24700.035.7797931
GTGAGCG3351.8189894E-1235.6018170
TGTGTCG7950.033.75454370
ACAGACG5100.033.1294670
CAGTTCG27200.032.52046270
GAGCAAA31250.032.12232270
CACGAGA1400.031.56761721
TATCAAC28550.030.938622
Produced by FastQC (version 0.11.5)