FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335484
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA60830.45549029415552716No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58630.43901686579547194No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGAATAAAACACT56040.4196231478624978No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTG51960.38907242617657717No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44500.3332125281920263No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT41760.31269562196177564No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC39160.2932270248089831No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC35220.26372461220052057No Hit
GTTTTATTCAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA33170.24837437213774183No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32890.2462777539828257No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA32700.24485504880627545No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32180.240961329375717No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29970.2244130217958433No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC29170.21842268421036867No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA29010.21722461669327375No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC28980.21699997903381843No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG28360.21235746740507563No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT26490.19835505329902867No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26370.19745650266120746No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTA25530.19116664819645912No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25510.19101688975682224No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA25490.19086713131718536No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA25130.1881714794037218No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25030.18742268720553745No Hit
GTCTCCAGACTCCCTGGCTGTGTATCTGGGCGAGAGGGCCACCATCAACTGCAAGTGCAGCCAGAGTGTTTTATT24960.18689853266680845No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC24880.18629949890826097No Hit
GAATAAAACACTCTGGCTGCACTTGCAGTTGATGGTGGCCCTCTCGCCCAGATACACAGCCAGGGAGTCTGGA24450.18307969245606837No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAACCTGCAGGCTGAAGAT24000.17971012756423888No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG23520.1761159250129541No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22570.16900239913020298No Hit
CTCCCTGGCTGTGTATCTGGGCGAGAGGGCCACCATCAACTGCAAGTGCAGCCAGAGTGTTTTATTCAGCTCCAA22380.16757969395365277No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTG22160.16593235111764723No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21970.16450964594109702No Hit
GTGTATCTGGGCGAGAGGGCCACCATCAACTGCAAGTGCAGCCAGAGTGTTTTATTCAGCTCCAACAATAAGAA21470.16076568495017537No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTG20750.1553743811232482No Hit
GATTTCACTCTCACCATCAGCAACCTGCAGGCTGAAGATGTGGCAATTTATTACTGTCAGCAATTTTATGATACT20620.15440095126560857No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA20560.15395167594669798No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT20420.15290336686923992No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA20270.1517801785719634No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19770.14803621758104177No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19670.14728742538285747No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAACC19490.14593959942612564No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT19090.14294443063338833No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18990.14219563843520402No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGGTGAGGAAGATGCTGGCAAAGGATGG18770.14054829559919851No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC18490.13845167744428238No Hit
GTAATAAATTGCCACATCTTCAGCCTGCAGGTTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA18330.13725360992718746No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17940.13433332035426856No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17920.13418356191463168No Hit
GTAATATGTGGCTGTGTCCACAGGGTCCATGTTGGTCATTGTAAGGATCACCTGGTTTTTGGAAGTGTCCTTGGT17880.13388404503535797No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA17290.12946617106607042No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA17220.12894201652734139No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAACCTGCAGGCTGAAGAT17080.12789370744988332No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17020.12744443213097273No Hit
ATTTATTACTGTCAGCAATTTTATGATACTCTCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA16950.1269202775922437No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTATCTGGGCGAGAGG16700.1250482970967829No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16680.12489853865714602No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGG16570.12407486723914325No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16490.12347583348059579No Hit
TCTTCAGCCTGCAGGTTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC16460.1232511958211405No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16340.1223526451833193No Hit
CAGTAATAAATTGCCACATCTTCAGCCTGCAGGTTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA16330.12227776596350087No Hit
GGCTGAAGATGTGGCAATTTATTACTGTCAGCAATTTTATGATACTCTCCTCACTTTCGGCGGAGGGACCAAGGT16320.12220288674368245No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG16250.12167873220495341No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16180.12115457766622437No Hit
GTATCTGGGCGAGAGGGCCACCATCAACTGCAAGTGCAGCCAGAGTGTTTTATTCAGCTCCAACAATAAGAACTA16080.12040578546804005No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15980.11965699326985572No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTT15710.11763525433475804No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15540.11636230759784467No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT15520.11621254915820782No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15300.1145652063222023No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTATCTGGGCGAGAGGGCCACCATCAACT15200.11381641412401795No Hit
CCCTGGCTGTGTATCTGGGCGAGAGGGCCACCATCAACTGCAAGTGCAGC14980.11216907128801243No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14960.11201931284837557No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG14660.10977293625382258No Hit
GAGTATCATAAAATTGCTGACAGTAATAAATTGCCACATCTTCAGCCTGCAGGTTGCTGATGGTGAGAGTGAAAT14570.10909902327545669No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAACCTGCAGGCTGAAGATG14460.10827535185745393No Hit
GCATTAGTGGAGTGGGTGTGGGCTGGATCCGTCAGCCCCCAGGAAAGGCCCTAGAGTGGCTTGCAGTCATTTATT14450.1082004726376355No Hit
CACCATCAGCAACCTGCAGGCTGAAGATGTGGCAATTTATTACTGTCAGCAATTTTATGATACTCTCCTCACTTT14070.10535506228453505No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCA13870.10385747788816638No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13620.10198549739270557No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA13510.1011618259747028No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGAT13490.10101206753506593No Hit
ATGATACTCTCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA13390.10026327533688162No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13360.10003863767742631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATTA951.891749E-10101.8755970
CGAATTT500.001133336782.9558370
GACTGTT700.004303834459.2541770
AATGGTA4100.057.32720670
GTGGTAT3100.056.993051
TATTCTA1556.5392305E-753.51989470
TGGTATC3300.051.4276772
TGTTGTA2151.2052624E-745.01479370
GGTATCA10450.041.9424361
ACTTGCA21000.039.50277770
AGGTACG350.001460312738.7911642
AGCGCTT6500.038.28730870
GTATCAA25250.036.331441
GAGCAAA38050.034.15614770
GTATACC400.00279856633.9766241
GGTACGG400.002816614533.9321023
TCTTGTT6450.032.15342770
GACAGGC2550.031.9779991
TATCAAC28700.031.8134842
ATCAACG29350.030.9839953
Produced by FastQC (version 0.11.5)