FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1151896
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCGCAGCGGTCACAGAGTGCATCTGCAGAGAGAACTGATTCTTGGCCGTGTCTACTGA45840.39795259294241836No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG42300.36722065186440445No Hit
GCCTTGGGCTGACCTAGGACGATCACCTTAGTCCCTCCGCCGAAGACCACACCAACGCTCCGTCTGCTATCCCA30670.26625667594991215No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28320.24585552862411192No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTTCTCGATGGGGGCTGGGAGGGCTTTG26710.23187857237111686No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26460.2297082375492232No Hit
GGACTAAGGTGATCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG26290.2282324098703355No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG25940.2251939411196844No Hit
GTATATTACTGTGCGACAACCCCCATAGAGATGGCCACCGTTTGGTCCTTTGATTACTGGGGCCAGGGAGCCCT25400.22050601790439417No Hit
CCTTAGTCCCTCCGCCGAAGACCACACCAACGCTCCGTCTGCTATCCCATGTTCCGCAGTAATAATCGCCCTCG25280.21946425718988521No Hit
GACCTAGGACGATCACCTTAGTCCCTCCGCCGAAGACCACACCAACGCTC24260.21060929111655913No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT23140.20088619111447562No Hit
CCCTAGACATCAACGGACTCCAGACTGCGGACGAGGGCGATTATTACTGCGGAACATGGGATAGCAGACGGAGCG22740.1974136553994458No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22260.19324661254141No Hit
GTTCCAGGGAGTTGCTGGTACCAGGATACATAATTAGTCCCAATGTCGGA21650.1879509955759895No Hit
GTGTCTGGTGCCTCCATCAGTAGTGGCTATTGGAGTTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAATTCAT21520.18682242146860478No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTT21310.18499934021821415No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21030.18256856521769324No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20650.17926965628841493No Hit
CACCTGCACTGTGTCTGGTGCCTCCATCAGTAGTGGCTATTGGAGTTGGATCCGGCAGCCCCCAGGGAAGGGA20620.17900921610978768No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCT20210.17544986700188211No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG19840.17223777146547953No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC19750.1714564509295978No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19040.16529270003541988No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCACCTTAGTCCCTC18990.16485863307104115No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGC18900.16407731253515942No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18590.1613860973560113No Hit
GTCTGGAGTCCGTTGATGTCTAGGGTGGCTGACGTGCCAGACTGGGAGCC18560.16112565717738406No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18410.15982345628424788No Hit
GTCTGGCACGTCAGCCACCCTAGACATCAACGGACTCCAGACTGCGGACGAGGGCGATTATTACTGCGGAACA18150.1575663080694785No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG17960.15591685360483934No Hit
GTTTGGGGGCTGTTCCAGGGAGTTGCTGGTACCAGGATACATAATTAGTC17700.15365970539006996No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG17460.15157618396105205No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC17180.14914540896053116No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC17030.14784320806739498No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16340.1418530839589685No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16250.1410717634230868No Hit
GTAATAATCGCCCTCGTCCGCAGTCTGGAGTCCGTTGATGTCTAGGGTGG16160.1402904428872051No Hit
AGGCAGGACTCAGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGG15610.13551570627903908No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15530.1348211991360331No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGGCAGGACTCAGGACAATCTTCATCA15490.13447394556453013No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT15300.13282449109989097No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15290.13273767770701522No Hit
CTATGGGGGTTGTCGCACAGTAATATACGGCCGTGTCCGCAGCGGTCACAGAGTGCATCTGCAGAGAGAACTGAT15290.13273767770701522No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15120.13126185002812754No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15060.13074096967087306No Hit
CCCTGGAACAGCCCCCAAACTCCTCATTTATTATACTAATAGGCGACCCTCAGGGATTCCCGACCGATTCTC14820.12865744824185515No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14790.12839700806322793No Hit
ACTCCAGACTGCGGACGAGGGCGATTATTACTGCGGAACATGGGATAGCAGACGGAGCGTTGGTGTGGTCTTCGG14700.12761568752734623No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGT14480.12570579288407982No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAG14250.12370908484793765No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14210.12336183127643469No Hit
CCCCCATAGAGATGGCCACCGTTTGGTCCTTTGATTACTGGGGCCAGGGAGCCCTTGTCACCGTCTCCTCAGCC14080.12223325716904998No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGATACTCCGACATTGGGACTAATTATGTATCCTGGTACCAGCAA14050.12197281699042274No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13900.12067061609728656No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG13760.11945522859702612No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13690.1188475348468959No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCACCTTAGTCCCTCCGCCGAAGAC13410.11641675984637502No Hit
ACCCTAGACATCAACGGACTCCAGACTGCGGACGAGGGCGATTATTACTG13300.11546181252474182No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13290.11537499913186608No Hit
GGATAGCAGACGGAGCGTTGGTGTGGTCTTCGGCGGAGGGACTAAGGTGATCGTCCTAGGTCAGCCCAAGGCTGC13270.11520137234611458No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13010.1129442241313452No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC12880.11181565002396049No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT12870.11172883663108475No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12490.1084299277018064No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12440.10799586073742767No Hit
GGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGGCCCAG12430.10790904734455194No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC12240.10625959287991277No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTG12220.10608596609416128No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12110.10513101877252808No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11840.10278705716488294No Hit
ACATGGGATAGCAGACGGAGCGTTGGTGTGGTCTTCGGCGGAGGGACTAA11750.10200573662900123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2050.0115.47346570
AACCCCG550.00533021656.1392770
GGCGAAT2250.045.5480532
GTGGTAT1800.045.4649661
GGTATCA6750.044.95981
GCGAATC2150.044.50816333
CGAATCT2000.044.44609534
ATCTCCG2550.044.10285610
AATCTCG2100.043.98832736
ACGAGAC2500.043.6236322
AAGGGGG3050.043.4396969
TGGTATC1900.043.0496372
AGGCGAA2500.042.3467931
GAATCTC2200.041.97239335
CGAGACT2650.041.1579423
TCTCGTA2000.041.11131338
CTTATAC2750.040.918471
TCGTATG2100.040.8474240
CGTATGC2200.040.57517241
TCGAAAC350.001336725239.49487349
Produced by FastQC (version 0.11.5)