FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004685285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1151896
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCGCAGCGGTCACAGAGTGCATCTGCAGAGAGAACTGATTCTTGGCCGTGTCTACT43190.37494704383034577No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT39370.34178432775181095No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27500.23873683040830074No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27110.23535110808614668No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG27070.23500385451464367No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG26240.22779834290595677No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25840.22432580719092696No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25660.22276316611916352No Hit
GCCTTGGGCTGACCTAGGACGATCACCTTAGTCCCTCCGCCGAAGACCACACCAACGCTCCGTCTGCTATCCCAT24840.21564446790335237No Hit
GGACTAAGGTGATCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGA24370.21156423843819236No Hit
GTATATTACTGTGCGACAACCCCCATAGAGATGGCCACCGTTTGGTCCTTTGATTACTGGGGCCAGGGAGCCCTT24140.2095675304020502No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21530.18690923486148056No Hit
CACCTGCACTGTGTCTGGTGCCTCCATCAGTAGTGGCTATTGGAGTTGGATCCGGCAGCCCCCAGGGAAGG21530.18690923486148056No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCT20990.1822213116461903No Hit
GTGTCTGGTGCCTCCATCAGTAGTGGCTATTGGAGTTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAATTCATT20280.17605756075201234No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20090.17440810628737316No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20000.17362678575149143No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA19920.17293227860848548No Hit
GTTCCAGGGAGTTGCTGGTACCAGGATACATAATTAGTCCCAATGTCGGAGTATCTTCCAGAGCAGGAGATGGT19740.17136963753672207No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA19710.17110919735809485No Hit
CCCTAGACATCAACGGACTCCAGACTGCGGACGAGGGCGATTATTACTGC19580.16998062325071014No Hit
CCTTAGTCCCTCCGCCGAAGACCACACCAACGCTCCGTCTGCTATCCCATGTTCCGCAGTAATAATCGCCCTCGT19220.1668553411071833No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18970.16468500628528965No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAA18270.15860806878398745No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTC17340.1505344232465431No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGG17300.1501871696750401No Hit
GACCTAGGACGATCACCTTAGTCCCTCCGCCGAAGACCACACCAACGCTC16940.14706188753151325No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC16850.14628056699563155No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTG16730.1452388062811226No Hit
GTCTGGCACGTCAGCCACCCTAGACATCAACGGACTCCAGACTGCGGACGAGGGCGATTATTACTGCGGAACATG16610.14419704556661364No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCACCTTAGTCCCTC16500.14324209824498044No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGA16410.14246077770909873No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGGAAGATACTCC16250.1410717634230868No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTT16180.1404640696729566No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16110.13985637592282635No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15810.137251974136554No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT15750.1367310937792995No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA15670.13603658663629356No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGGCAGGACTCAGGACAATCTTCATCAT15640.13577614645766634No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15300.13282449109989097No Hit
AGGCAGGACTCAGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT15200.13195635717113352No Hit
GTCTGGAGTCCGTTGATGTCTAGGGTGGCTGACGTGCCAGACTGGGAGCCAGAGAATCGGTCGGGAATCCCTGAG15120.13126185002812754No Hit
CTATGGGGGTTGTCGCACAGTAATATACGGCCGTGTCCGCAGCGGTCACA14810.12857063484897943No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCT14800.12848382145610368No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG14620.12692118038434025No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTT14020.12171237681179552No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13950.12110468306166529No Hit
GTTTGGGGGCTGTTCCAGGGAGTTGCTGGTACCAGGATACATAATTAGTC13910.1207574294901623No Hit
CCCTGGAACAGCCCCCAAACTCCTCATTTATTATACTAATAGGCGACCCTCAGGGATTCCCGACCGATTCTCTG13820.1199761089542806No Hit
ACCCTAGACATCAACGGACTCCAGACTGCGGACGAGGGCGATTATTACTGCGGAACATGGGATAGCAGACGGAGC13620.11823984109676569No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGATACTCCGACATTGGGACTAATTATGTATCCTGGTACCAGCAA13550.11763214734663546No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13430.11659038663212652No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC13280.11528818573899033No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT13240.11494093216748735No Hit
ACTCCAGACTGCGGACGAGGGCGATTATTACTGCGGAACATGGGATAGCAGACGGAGCGTTGGTGTGGTCTTCG13190.11450686520310863No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCACCTTAGTCCCTCCGCCGAAGAC13180.11442005181023288No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGAT13040.11320466430997243No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGG13010.1129442241313452No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTT12930.11224971698833922No Hit
GGATAGCAGACGGAGCGTTGGTGTGGTCTTCGGCGGAGGGACTAAGGTGATCGTCCTAGGTCAGCCCAAGGCTGC12860.111642023238209No Hit
GGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGGCCCAGT12810.11120795627383027No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT12610.10947168841631537No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCA12500.10851674109468216No Hit
CCCCCATAGAGATGGCCACCGTTTGGTCCTTTGATTACTGGGGCCAGGGAGCCCTTGTCACCGTCTCCTCAGCCT12400.10764860716592471No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC12220.10608596609416128No Hit
ACTCAGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGGC12120.10521783216540383No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG12110.10513101877252808No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGG11940.1036551910936404No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGG11890.10322112412926167No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA11890.10322112412926167No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11710.10165848305749825No Hit
GAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGGCAGGACTCAGGACAATCTTCATCATGACCTGCTCCC11580.10052990895011356No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)