FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004685286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1139194
Sequences flagged as poor quality0
Sequence length19-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATGAGCCTCATCCTCAGACTGGAGCCCACGGATGGCCAGGGAGG79280.695930631657119No Hit
CATTATTACTGTGCGACATGGGATGACAGCCTGAATGGTCCGGTATTCGG66340.5823415502539514No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG40590.3563045451433206No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGG40220.3530566347786242No Hit
GTAATAGAAGGCGGTGTCCTCAGTTTTCAGATTGTTCATTTGGAGAAACA40070.351739914360504No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34720.3047768861142176No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTG28800.2528103202790745No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG28070.24640228091088964No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC27780.24385662143585726No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGGACCATTCAGG27720.24332993326860922No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT25900.22735372552875102No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCA25350.22252575066231037No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT24270.2130453636518451No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24140.2119042059561409No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23890.20970967192594062No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22950.2014582239723875No Hit
TTCTATTACTGTGCAAGAAGGGTGTCTGATTTTGGTGGTTACTATGACTA22850.20058041036030738No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22490.19742028135681894No Hit
TGACTATACCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGGTCTGGACTGGGTCTCTCTAATTAGTTGGGATGG22400.19663024910594684No Hit
GGTCTGGACTGGGTCTCTCTAATTAGTTGGGATGGTGGTACCACATCCTA21710.190573335182594No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21460.18837880115239372No Hit
TTATTACTGTGCGACATGGGATGACAGCCTGAATGGTCCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGG21300.18697429937306553No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20190.17723056827897618No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG20070.17617719194448006No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19710.17301706294099162No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTGATGACTATACCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGG19550.17161256116166343No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19270.1691546830478391No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG19050.16722349310126283No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18810.16511674043227054No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18580.16309776912448626No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18540.1627466436796542No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18500.16239551823482218No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18370.161254360539118No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC18250.16020098420462187No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18240.16011320284341385No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC17630.15475853980972512No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC17570.15423185164247705No Hit
CCCCTGAGGGCCGCTGATTATCACTAAAGGAGAGGAGTTTGGGGGCCGTCCCTGGGAGCTGCTGGTACCAGTTTA17500.15361738211402098No Hit
CAGTAATAATGAGCCTCATCCTCAGACTGGAGCCCACGGATGGCCAGGGA17460.15326625666918892No Hit
GCACTGAACACAGAGAACGCACCATGGAGTCTGGACTGAGCTGGGTTTTC17450.1531784753079809No Hit
TCCTCAGACTGGAGCCCACGGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC17440.15309069394677288No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17400.15273956850194084No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA16960.14887718860878832No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16760.14712156138462806No Hit
GTATTACTTCCGACGTTGGCGGTGCTTCCAGAACAAGAGATGGTGACCCT16730.14685821730100404No Hit
GTCTGAGGATGAGGCTCATTATTACTGTGCGACATGGGATGACAGCCTGAATGGTCCGGTATTCGGCGGAGGGAC16730.14685821730100404No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16610.1458048409665079No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16340.14343474421389157No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC16250.14264471196301945No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16110.14141577290610732No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG15870.139309020237115No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT15660.13746561165174676No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15590.1368511421232907No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGGACCATTCAGGC15510.13614889123362658No Hit
GTCCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC15490.13597332851121055No Hit
CAGTAATAGAAGGCGGTGTCCTCAGTTTTCAGATTGTTCATTTGGAGAAACAAGGAGTTTTTGCTGTTGTCTC15410.13527107762154644No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG15330.13456882673188236No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT15280.1341299199258423No Hit
CTATTACTGTGCAAGAAGGGTGTCTGATTTTGGTGGTTACTATGACTACTGGGGCCAGGGAACCCTGGTCACCG14860.1304431027551058No Hit
GTCCAGACCCTTCCCCGGAGCTTGACGGACCCAGTGCATGGTATAGTCAT14750.12947750778181769No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG14670.12877525689215358No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG14630.12842413144732154No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC14400.12640516013953726No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14360.12605403469470522No Hit
GGGTTCCCTGGCCCCAGTAGTCATAGTAACCACCAAAATCAGACACCCTTCTTGCACAGTAATAGAAGGCGGTG14360.12605403469470522No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14320.12570290924987315No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG14220.12482509563779304No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG14100.12377171930329689No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG14030.1231572497748408No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAG13820.12131384118947254No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCC13740.12061159029980845No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13730.12052380893860044No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13510.11859261899202418No Hit
ACCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGGTCTGGACTGGGTCTC13470.11824149354719214No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC13470.11824149354719214No Hit
GAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGGACGGCCCCCAAA13260.11639808496182388No Hit
ACCACATCCTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACAGCAAAAACTCCTTGTTTCTC13240.11622252223940786No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13210.11595917815578381No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAG13180.11569583407215979No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGGAC13160.11552027134974377No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA13050.11455467637645562No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT13030.1143791136540396No Hit
GTCTGGGGGAGTCGTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTGATGA12980.11394020684799955No Hit
ATACCGGACCATTCAGGCTGTCATCCCATGTCGCACAGTAATAATGAGCCTCATCCTCAGACTGGAGCCCACGGA12840.1127112677910874No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12830.11262348642987936No Hit
GTATAGTCATCAAAGGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGACCCCCCAGGCTGTACCACGACTCCC12800.11236014234625533No Hit
CCTTTAGTGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC12630.11086785920571914No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCCGTGGGCTCCAG12410.10893666925914287No Hit
GATCAGCACTGAACACAGAGAACGCACCATGGAGTCTGGACTGAGCTGGGTTTTCCTTGTTGTTATTTTAAAAGG12350.10840998109189479No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12350.10840998109189479No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12300.10797107428585474No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12290.10788329292464671No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12110.1063032284229025No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12090.10612766570048648No Hit
GTGGTACCACATCCTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACAGCAAAAACTCCTTGT12070.10595210297807045No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT11960.10498650800478233No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC11930.1047231639211583No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTGATGACTATACCATGCACTGGGTCCG11830.10384535030907817No Hit
GAACACAGAGAACGCACCATGGAGTCTGGACTGAGCTGGGTTTTCCTTGTTGTTATTTTAAAAGGTGTCCAGTG11810.10366978758666215No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTGTTTCCATTCGGTGA11790.10349422486424613No Hit
CTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACAGCAAAAACTCCTTGTTTCTCCAAATGAA11680.10252862989095798No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11640.10217750444612594No Hit
GAGTACGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTGT11620.10200194172370992No Hit
GGATGTGGTACCACCATCCCAACTAATTAGAGAGACCCAGTCCAGACCCTTCCCCGGAGCTTGACGGACCCAG11590.10173859764008589No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTG11570.10156303491766987No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCT11400.10007075177713366No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)