FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004685287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1139194
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTATTACTGTGCGACATGGGATGACAGCCTGAATGGTCCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA59620.5233524755221675No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC43380.380795544920356No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT39010.34243509007245476No Hit
GTAATAGAAGGCGGTGTCCTCAGTTTTCAGATTGTTCATTTGGAGAAACAAGGAGTTTTTGCTGTTGTCTCTGGA36640.3216309074661559No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT33790.2966132195218725No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32280.2833582339794627No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28270.24815790813504987No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC25680.2254225355821748No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCA25390.2228768761071424No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22590.19829809496889908No Hit
TGACTATACCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGGTCTGGACT22040.1934701201024584No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22010.19320677601883438No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21880.1920656183231302No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATA20990.18425307717561715No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG20960.1839897330919931No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20880.18328748220232902No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGGACCATTCAGGCTGTCATCCCATGTC20270.1779328191686403No Hit
TTATTACTGTGCGACATGGGATGACAGCCTGAATGGTCCGGTATTCGGCG20000.17556272241602397No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG19950.1751238156099839No Hit
TTCTATTACTGTGCAAGAAGGGTGTCTGATTTTGGTGGTTACTATGACTACTGGGGCCAGGGAACCCTGGTCACC19770.1735437511082397No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19640.17240259341253553No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC19630.1723148120513275No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTGATGACTATACCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGGT19520.17134921707803938No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19430.17055918482716728No Hit
GGTCTGGACTGGGTCTCTCTAATTAGTTGGGATGGTGGTACCACATCCTA19340.16976915257629516No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19240.16889133896421507No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA19200.168540213519383No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19100.1676623999073029No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG19080.16748683718488686No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17900.15712863656234144No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17640.15484632117093314No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT17640.15484632117093314No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG17270.1515984108062367No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT17210.15107172263898863No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17170.15072059719415656No Hit
GTATTACTTCCGACGTTGGCGGTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACGCTGAG17160.15063281583294855No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17150.15054503447174053No Hit
GAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGGACGGCCCCCAAACTCCTCTCCTTTAGTGATAATCAGC17140.15045725311053254No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTG17040.14957943949845243No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16860.14799937499670818No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCA16830.14773603091308415No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16750.14703378002342007No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG16690.146507091856172No Hit
CCTTTAGTGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC16620.1458926223277159No Hit
GTCTGAGGATGAGGCTCATTATTACTGTGCGACATGGGATGACAGCCTGAATGGTCCGGTATTCGGCGGAGGGAC16440.1443125578259717No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCT16270.1428202746854355No Hit
CTCTTGTTCTGGAAGCACCGCCAACGTCGGAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGGACGGCCCC16190.1421180237957714No Hit
GCACTGAACACAGAGAACGCACCATGGAGTCTGGACTGAGCTGGGTTTTC15910.13966014568194707No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA15840.13904567615349098No Hit
CCCCTGAGGGCCGCTGATTATCACTAAAGGAGAGGAGTTTGGGGGCCGTCCCTGGGAGCTGCTGGTACCAGTTTA15810.13878233206986695No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA15730.13808008118020285No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG15280.1341299199258423No Hit
GTCCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC15130.13281319950772214No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG14970.13140869772839395No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG14970.13140869772839395No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG14790.12982863322664973No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG14680.1288630382533616No Hit
GGGTTCCCTGGCCCCAGTAGTCATAGTAACCACCAAAATCAGACACCCTTCTTGCACAGTAATAGAAGGCGGTGT14660.12868747553094556No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCCGTGGGCTCCAGT14630.12842413144732154No Hit
GTCCAGACCCTTCCCCGGAGCTTGACGGACCCAGTGCATGGTATAGTCATCAAAGGTGAATCCAGAGGCTGCACA14500.12728297375161737No Hit
GTCTGGGGGAGTCGTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTGAT14140.12412284474812894No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC13960.12254278024638472No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC13920.12219165480155268No Hit
CCCAGGGACGGCCCCCAAACTCCTCTCCTTTAGTGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTG13830.12140162255068057No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAG13690.1201726834937684No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG13620.11955821396531233No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT13530.11876818171444022No Hit
CTATTACTGTGCAAGAAGGGTGTCTGATTTTGGTGGTTACTATGACTACTGGGGCCAGGGAACCCTGGTCACCGT13500.11850483763081616No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAAGACCTTGGG13370.11736367993511203No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGGACCATTCAGG13280.1165736476842399No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTT13260.11639808496182388No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT13220.11604695951699184No Hit
ACCACATCCTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACAGCAAAAACTCCTTGTTTCTC13140.11534470862732775No Hit
CAGTAATAGAAGGCGGTGTCCTCAGTTTTCAGATTGTTCATTTGGAGAAACAAGGAGTTTTTGCTGTTGTCTCTG13010.11420355093162358No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC13000.11411576957041558No Hit
ACCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGGTCTGGACTGGGTCTC12990.11402798820920756No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGGT12920.11341351868075149No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG12740.11183345417900725No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG12650.11104342192813516No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC12490.10963892014880697No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT12480.10955113878759895No Hit
GTGGTACCACATCCTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCC12390.10876110653672685No Hit
GATCAGCACTGAACACAGAGAACGCACCATGGAGTCTGGACTGAGCTGGG12380.10867332517551884No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12380.10867332517551884No Hit
GTATAGTCATCAAAGGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGACCCCCCAGGCTGTACCACGACTCCC12360.1084977624531028No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGC12350.10840998109189479No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT12350.10840998109189479No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC12180.1069176979513586No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGGT12040.10568875889444643No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTG11930.1047231639211583No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCCGTGGGCTCCAGTCTGAGGATGAGGCTCATTATTACTGTGCGACATG11920.1046353825599503No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCGGCAAGTCCGTGACATGCCACGT11920.1046353825599503No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11900.10445981983753425No Hit
GTAATAATGAGCCTCATCCTCAGACTGGAGCCCACGGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGA11840.10393313167028617No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGC11820.10375756894787015No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11710.10279197397458203No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11690.10261641125216601No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC11560.10147525355646185No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA11530.1012119094728378No Hit
AGTCTGGACTGAGCTGGGTTTTCCTTGTTGTTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGAGGAGTCTG11420.10024631449954968No Hit
GGATGTGGTACCACCATCCCAACTAATTAGAGAGACCCAGTCCAGACCCTTCCCCGGAGCTTGACGGACCCAGT11410.10015853313834168No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11400.10007075177713366No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)