FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004685289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459073
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTATTCTCCGGGGTACAGTTTTGGCCAGGGGACCAAGCTCG27150.5914092094285659No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC26920.586399112995101No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20710.45112650929155057No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCTTGATCTATAAGGCGT17580.38294563174048574No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA17340.377717705027305No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13340.29058559314096016No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT12980.2827437030711891No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC12510.2725056799245436No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12170.26509945041420424No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC11280.24571255551949253No Hit
GTTTTGGCCAGGGGACCAAGCTCGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCT11060.24092028936574358No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10260.2234938669884746No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10160.22131556419131598No Hit
GGCCAAAACTGTACCCCGGAGAATAACTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAG10150.22109773391160012No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9890.2154341466389877No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9830.21412716496069253No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC9690.21107754104467044No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA9540.20781008684893254No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9280.2021464995763201No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA9270.20192866929660425No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG9070.197572063702287No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA8980.19561159118484422No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATA8770.19103715531081114No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA8690.18929451307308423No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGT8540.1860270588773463No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT8520.18559139831791457No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8460.18428441663961942No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA8460.18428441663961942No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCTTGATCTATAAGGCGTCTA8170.1779673385278594No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8090.17622469629013252No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7940.17295724209439456No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA7930.17273941181467872No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA7840.17077893929723595No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG7360.16032308587087457No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGGCGGTGCTGATGGACTTGTCGGCTGA7360.16032308587087457No Hit
GTGCAGCCACAGTTCGTTTGATCTCGAGCTTGGTCCCCTGGCCAAAACTGTACCCCGGAGAATAACTATTATA7350.1601052555911587No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC7130.15531298943740973No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT6930.15095638384309248No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6800.14812459020678628No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCGAGCTTGGTCC6770.1474710993676387No Hit
GTACAGTTTTGGCCAGGGGACCAAGCTCGAGATCAAACGAACTGTGGCTG6760.14725326908792283No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6720.14638194796905937No Hit
CAGTATAATAGTTATTCTCCGGGGTACAGTTTTGGCCAGGGGACCAAGCTCGAGATCAAACGAACTGTGGCTGCA6690.14572845712991178No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6490.14137185153559456No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6440.14028270013701524No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGG6420.13984703957758352No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC6090.13265864034696007No Hit
CCTTATAGATCAAGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTAATACTCT6010.13091599810923318No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTAATACTCTGACTGGCCCGGCAAGT6000.13069816782951732No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC5950.129609016430938No Hit
GTATTACTGTGCGAGACCCCACAGCTATGGAACGGACCCGTTTGACTACTGGGGCCAGGGAACCCTCGTCACCGT5910.12873769531207455No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA5910.12873769531207455No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA5610.12220278692059867No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA5610.12220278692059867No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTA5590.12176712636116696No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATAGATCAAGAGGTTAGGGGCTTTCCCTGGTTTCT5580.1215492960814511No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA5570.12133146580173522No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5570.12133146580173522No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT5560.12111363552201938No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG5460.11893533272486076No Hit
ATGTATTACTGTGCGAGACCCCACAGCTATGGAACGGACCCGTTTGACTACTGGGGCCAGGGAACCCTCGTCACC5360.11675702992770212No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5290.11523221796969109No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT5270.11479655741025938No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC5200.11327174545224833No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCC5200.11327174545224833No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC5130.1117469334942373No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA5080.11065778209565799No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT5020.10935080041736282No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA5020.10935080041736282No Hit
TAGTTATTCTCCGGGGTACAGTTTTGGCCAGGGGACCAAGCTCGAGATCAAACGAACTGTGGCTGCACCAT5000.10891513985793108No Hit
CAGTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGGCGGTGCTGATGGACTTGTCG4960.10804381873906765No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCA4950.10782598845935178No Hit
ACTTATTACTGCCAACAGTATAATAGTTATTCTCCGGGGTACAGTTTTGGCCAGGGGACCAAGCTCGAGATCAAA4950.10782598845935178No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGAT4930.10739032789992006No Hit
AAACTGTACCCCGGAGAATAACTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCT4890.10651900678105661No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC4860.10586551594190903No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC4840.10542985538247729No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTT4820.10499419482304558No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCTTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCAT4810.1047763645433297No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTT4600.10020192866929661No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)