FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093416
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTATTACTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTCATCTATAAGGCG56700.5185583529050243No Hit
GTATAATGGTTTTTCTCGATGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCA51010.46651960461526076No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48000.43899119822647553No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT46370.4240837887867015No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35200.32192687869941544No Hit
CCATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGTTGATGGTGAGAGTGAATTCTGTC33450.30592199126407515No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT31580.2888196258331687No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29870.2731805643963505No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29690.27153434740300125No Hit
GTAATAGACAGCCGTGTCCTCGTCTCTCAGACTGTTCATTTGCAAATACA29020.2654067619277567No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28850.2638520014340379No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC28820.26357763193514633No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCAC28810.2634861754355159No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26910.24610944050571787No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCGTCACTTGCCGGGCCAGTCAGAGTATTACTACCTGGTTGGCCTGGTA26890.24592652750645683No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26660.24382302801495498No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25170.2301960095700081No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC24370.22287948959956688No Hit
GAATTCACTCTCACCATCAACAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAATGGTTTT23550.21538005662986456No Hit
GTATTACTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT22800.2085208191575759No Hit
CCTTATAGATGAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTAGTAATACTCTGA22700.20760625416127074No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22610.2067831456645961No Hit
GTAATACTCTGACTGGCCCGGCAAGTGACGGTGACTCTGTCTCCCACAGA22030.2014786686860262No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22000.20120429918713462No Hit
CCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCGTCACTTGCCGGGCCAGTCAGAGTATTACTACCTGG21920.2004726471900905No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCATCGAGAAAAACCATTATACTGT21850.19983245169267688No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21590.1974545827022835No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT21240.19425360521521542No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20550.18794310674070985No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC19920.18218134726398738No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19770.18080949976952962No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC19350.17696832678504795No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACC19040.174133175296502No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18540.16956035031497618No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTAGTAATACTCTGACTGGCCCGGCAAGTGA17970.16434732983603678No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATG17850.16324985184047058No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG17350.1586770268589448No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16740.15309818038148335No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16240.14852535539995756No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16150.14770224690328293No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGG15310.1400199009343196No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15300.13992844443468908No Hit
CACCATCAACAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAAC15260.13956261843616702No Hit
CCCCTAAGCTCCTCATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATC15250.1394711619365365No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15120.1382822274413398No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATAGA14780.13517270645390225No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14490.1325204679646173No Hit
GTAGTAATACTCTGACTGGCCCGGCAAGTGACGGTGACTCTGTCTCCCACAGATGCAGACAGGGTGGAAGGAGA14460.13224609846572574No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACCTCCAGATGACCCAGTCTCCTTCCACC14440.13206318546646473No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14310.130874250971268No Hit
ACTTATTACTGCCAACAGTATAATGGTTTTTCTCGATGGACGTTCGGCCA14120.12913657747828822No Hit
GTGGGAGACAGAGTCACCGTCACTTGCCGGGCCAGTCAGAGTATTACTACCTGGTTGGCCTGGTATCAGCAGAAA14050.12849638198087462No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13590.12428938299787089No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCGTCACTTGCCGGGCCAGTCAGAGTATTACTAC13560.12401501349897934No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA13490.12337481800156573No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG13460.12310044850267418No Hit
GTTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAACTAGA13370.12227734000599955No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG13350.12209442700673852No Hit
GTCTATTACTGTGCAAGAGAGGCGAATTACGATTCTTGGAGTGGAGTGAA13340.122002970507108No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATAGATGAGGAGCTTAGGGGCTTTCCCTGGTTTC13330.12191151400747749No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13240.12108840551080284No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13120.11999092751523664No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13040.11925927551819254No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12880.11779597152410427No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT12870.11770451502447375No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12840.11743014552558222No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT12670.11587538503186345No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12530.11459499403703621No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG12400.11340605954183952No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12390.113314603042209No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12280.11230858154627334No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12260.1121256685470123No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12220.11175984254849024No Hit
CCATCAACAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAATGGTTTTTCTCGATGGACGT12200.11157692954922921No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAACAGCCTGCAGCCTGATGATTT12120.11084527755218507No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC11830.10819303906290012No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTCATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCA11740.10736993056622549No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC11650.10654682206955082No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11620.1062724525706593No Hit
CAGTATAATGGTTTTTCTCGATGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCA11520.10535788757435413No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11510.10526643107472361No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11430.1045347790776795No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGG11330.10362021408137434No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11270.10307147508359124No Hit
GTGCCAAATGTGACCTCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCGTCA11150.10197399708802506No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGTTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG10960.10023632359504525No Hit
CCTTGGTCCCTTGGCCGAACGTCCATCGAGAAAAACCATTATACTGTTGG10960.10023632359504525No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG150.002591048167.3612310
GTTATTA753.253656E-452.60436670
GTGGTAT2450.052.2488821
TCGTATG1800.043.74857740
CGTATGC2000.042.8672741
GTATGCC2200.040.59142742
ATCTCGT1950.040.23740437
GCGTAGA29700.040.18388770
AATGGTA4000.039.45327870
ACGAGAC2350.038.7677922
GCCGTCT2050.038.73753446
CGAGACA2450.038.5821223
ATGCCGT2200.037.56887444
TCCGAGA6050.037.49691470
GTATCAA23800.037.2253041
CACGAGA2500.036.421721
TACACAT2800.034.8834955
TGTGTCG9150.034.49466770
TGTTAGA15600.034.14225870
TGCCGTC2350.033.7385445
Produced by FastQC (version 0.11.5)