FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093416
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTATTACTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTCATCTATAAGGCGT48090.4398143067231502No Hit
GTATAATGGTTTTTCTCGATGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT47870.43780226373127884No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47270.43231487375344796No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA45010.4116457048369514No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36020.32942631166911773No Hit
CCATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGTTGATGGTGAGAGTGAATTCTGTC33410.30555616526555307No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT31540.28845379983464664No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC29630.2709856084052181No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA28660.26211432794105816No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC26400.24144515902456157No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCGTCACTTGCCGGGCCAGTCAGA25930.23714670354192732No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC25720.2352261170496865No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT25530.2334884435567067No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25470.2329397045589236No Hit
GTAATAGACAGCCGTGTCCTCGTCTCTCAGACTGTTCATTTGCAAATACACCGTGTTCTTGGCGTTGTCTCTGGA24570.22470861959217717No Hit
GAATTCACTCTCACCATCAACAGCCTGCAGCCTGATGATTTTGCAACTTA23610.21592879562764766No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23260.2127278181405796No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22500.20577712416866042No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA22250.20349071167789753No Hit
CCTTATAGATGAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTAGTAATACTCT22210.20312488567937548No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22030.2014786686860262No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC21920.2004726471900905No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATA21820.19955808219378535No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21680.19827769119895813No Hit
GTATTACTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTCATCTATAAGGCGTCTA21470.1963571047067173No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21310.19489380071262905No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21150.1934304967185408No Hit
GTAATACTCTGACTGGCCCGGCAAGTGACGGTGACTCTGTCTCCCACAGATGCAGACAGGGTGGAAGGAGACTGG20860.19077825822925584No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA20790.19013806273184222No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCATCGAGAAAAACCATTATACTGTT20560.18803456324034035No Hit
CCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCGTCACTTGCCG20020.1830959122602925No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA19230.17587084878948178No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT19100.17468191429428506No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC18660.17065782831054238No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18260.16699956832532176No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17360.15876848335857532No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGT17360.15876848335857532No Hit
GTTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAACTA17150.1568478968663345No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTAGTAATACTCTGACTGGCCCGGCAAGT16930.15483585387446314No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGGTGAGGAAGATGCTGGCAAAGGATGG16700.1527323543829613No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACCT16440.15035448539256788No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15630.1429465089224961No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15590.14258068292397405No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15250.1394711619365365No Hit
GTGGGAGACAGAGTCACCGTCACTTGCCGGGCCAGTCAGAGTATTACTACCTGGTTGGCCTGGTATCAGCAGAAA14410.13178881596757316No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT14310.130874250971268No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14150.12941094697717978No Hit
GTAGTAATACTCTGACTGGCCCGGCAAGTGACGGTGACTCTGTCTCCCACAGATGCAGACAGGGTGGAAGGA14070.12867929498013564No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC13850.12666725198826428No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13680.12511249149454554No Hit
CCCCTAAGCTCCTCATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTG13580.12419792649824037No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACCTCCAGATGACCCAGTCTCCTTCCACC13500.12346627450119625No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13490.12337481800156573No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13480.12328336150193521No Hit
CACCATCAACAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAATGGTTTTTCTCGATGGAC13340.122002970507108No Hit
ACTTATTACTGCCAACAGTATAATGGTTTTTCTCGATGGACGTTCGGCCA13230.12099694901117233No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13170.12044821001338923No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA13150.1202652970141282No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12960.1185276235211484No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12800.11706431952706015No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATAGATGAGGAGCTTAGGGGCTTTCCCTGGTTTCT12740.11651558052927706No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCGTCACTTGCCGGGCCAGTCAGAGTATTACTAC12730.11642412402964654No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12650.11569247203260243No Hit
GTCTATTACTGTGCAAGAGAGGCGAATTACGATTCTTGGAGTGGAGTGAA12600.11523518953444985No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG12590.11514373303481931No Hit
GGGTGGAAGGAGACTGGGTCATCTGGAGGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGA12580.11505227653518879No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA12540.11468645053666673No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12510.11441208103777518No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12320.1126744075447954No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12270.11221712504664282No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12150.11111964705107663No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGTTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCC11890.10874177806068322No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACCTCCAGAT11780.10773575656474754No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11710.10709556106733394No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTGTC11630.1063639090702898No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA11370.1039860400798964No Hit
CCATCAACAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAG11230.10270564908506918No Hit
CAGTATAATGGTTTTTCTCGATGGACGTTCGGCCAAGGGACCAAGGTGGA11070.10124234509098091No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGT550.001571024176.400870
AATGGTA2850.058.9760570
TCCTCTA750.005370660756.0272570
CCGCTAT1607.4370655E-752.52554370
GAGTTTA200.00810999550.493329
GTTTGGT2252.764864E-949.80270
GTCGGCA850.00881017449.43580670
TATGGTT2003.4590212E-642.02043570
GAGCAAA29450.040.9027570
GTGGTAT3300.039.8226851
ATAGCGA459.6101634E-538.60295550
GTATCAA24400.037.7009851
AGTTGTT5650.037.18622670
TTCTTAA2706.4946835E-736.31395770
CCTGCTT5450.035.98080470
ATCAATT1600.002410291535.0170370
TTACACG400.002489991734.79521651
GTACGGA400.002914402233.6961171
CGATTCA4604.0017767E-1133.4945570
GCGTTGT6600.033.0032562
Produced by FastQC (version 0.11.5)