FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479810
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG63990.43242037829180774No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACATAAATATTCAGGCTGTTATCCCA55320.3738317757009346No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG45590.3080800913630804No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG43150.2915914880964448No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG43120.29138875936775666No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACATAAATATTC40080.2708455815273582No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT35810.24199052581074598No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAGCGCAGTATCAGGGAGAACTGATTCTTGGACGTGTCTACTGA35610.240639000952825No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC35590.24050384846703293No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC35350.23888201863752778No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC35000.23651685013616613No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30150.20374237233158313No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29360.19840384914279535No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG27860.18826741270838826No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG27660.1869158878504673No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC27100.18313161824828864No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26630.1799555348321744No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAG26000.17569823152972341No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25520.17245457187071314No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25140.16988667464066334No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24960.1686703022685345No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG24410.16495360890925187No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24110.16292632162237045No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCC23510.1588717470486076No Hit
GATTATTACTGCGGAACATGGGATAACAGCCTGAATATTTATGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTC23030.15562808738959732No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAACCTTCTCCAACATTGGGAG23000.15542535866090917No Hit
GTTTGGGGGCTGTTCCTGGGATCTGCTGGTACCAGGATACAAAATTACTC22980.15529020617511707No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC22950.15508747744642895No Hit
AGGCAGGACTCAGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGG22790.15400625756009217No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22620.15285746143085935No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT22240.15028956420080955No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGCC21240.1435319399112048No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACATAAATATTCA20940.1415046526243234No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGGCAGGACTCAGGACAATCTTCATCA20920.1413695001385313No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20820.14069373770957083No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20700.13988282279481823No Hit
GTATCAGGGAGAACTGATTCTTGGACGTGTCTACTGATATGGAGACTCGACTCCTGAGGGAGGGGTTGTAGTTGG20460.1382609929653131No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19750.13346307971969373No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG19740.1333955034767977No Hit
GGATAACAGCCTGAATATTTATGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGC19530.1319764023759807No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19330.13062487751805976No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCG19170.12954365763172299No Hit
GGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAACATGGGATAACAGCCTGAATATTTA19150.12940850514593089No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGG19030.1285975902311783No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGGAACCTTCTCCAACATTGGGAGTAAT18600.1256918117866483No Hit
GTCTATTACTGTGCGAGAGATATTGGATACAACTTTGGTTCAGGCTTTGG18390.12427271068583129No Hit
GTTCCTGGGATCTGCTGGTACCAGGATACAAAATTACTCCCAATGTTGGA18380.12420513444293524No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT18300.12366452449976686No Hit
GGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGGCCCAG18230.12319149079949453No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT18140.1225833046134301No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG17660.11933964495441983No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17610.11900176373993959No Hit
CCCTGATACTGCGCTCTGTGACCGCCGCAGACACGGCCGTCTATTACTGTGCGAGAGATATTGGATACAACTT17470.11805569633939492No Hit
GCCCCCAAACTCCTCATTTATGACACTAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAG17230.11643386650988978No Hit
GGGTGGCTGACGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGAATCCCTGAGGGTCGCTTATTAGTGTCATAAA16850.11386596927983998No Hit
ACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAACATGGGATAACAGCCTGAATATTTATGTGGTGTTCGG16740.11312263060798346No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16550.11183868199295854No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16450.11116291956399808No Hit
GTTATCCCATGTTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACG16240.10974381846318107No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16210.10954108973449295No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16000.10812198863367595No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15990.10805441239077991No Hit
ACTCAGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGGC15880.10731107371892337No Hit
CTAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAGCCACCCTGGGCATCA15830.10697319250444315No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA15600.10541893891783405No Hit
CTCCTGCTCTGGAACCTTCTCCAACATTGGGAGTAATTTTGTATCCTGGTACCAGCAGATCCCAGGAACAGCCCC15600.10541893891783405No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCT15520.10487832897466567No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC15470.10454044776018544No Hit
TGGGATAACAGCCTGAATATTTATGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG15380.103932261574121No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA15350.10372953284543285No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15260.10312134665936842No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15220.10285104168778424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3900.079.3746470
AATGGTA4450.052.7729170
TATCGGC200.00757419251.37484740
CGTCGGA3500.048.7978170
GGTATCA13600.046.5565451
TCGTATG3100.040.87890640
ATGGCAA3150.040.66483770
CTAGTCA8150.039.29271370
CGTATGC3250.039.0660441
GTATCAA33800.038.0634771
GTGGTAT3400.037.6414641
TGGTATC3500.037.516842
ATCTCGT3050.036.84199537
TATACAC3650.035.975053
TCTCGTA3300.035.16328438
TTATACA3850.034.9807362
CGAGACT3700.034.5906223
ACTAGGC3650.034.1914127
CACGAGA3750.034.1224921
TACACAT3750.034.119035
Produced by FastQC (version 0.11.5)