FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479810
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT60720.4103229468648002No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG43460.29368635162622225No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43020.2907129969387962No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACATAAATATTCAGGCTGTTATCCCAT42740.28882086213770686No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40180.2715213439563187No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG39230.2651016008811942No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCT38850.2625337036511444No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC35430.23942262858069616No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAGCGCAGTATCAGGGAGAACTGATTCTTGGACGTGTCTACT35110.23726018880802263No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAC34650.2341516816348045No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA31420.21232455517938115No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG31190.21077030159277205No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACATAAATATTC30270.20455328724633567No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29790.20130962758732543No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC29140.1969171717990823No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT29010.1960386806414337No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATA28770.19441685081192855No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA26390.17833370500266926No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG25650.17333306302836177No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC25440.17191396192754474No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAACCTTCTCCAACATTGGGAGTAATTTTGTATCCTGGTACCAGCA24830.16779181111088584No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTG23870.1613044917928653No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23790.16076388184969692No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT23770.16062872936390482No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22380.15123563160135423No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGGCAGGACTCAGGACAATCTTCATCAT22330.15089775038687397No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG21950.14832985315682418No Hit
AGGCAGGACTCAGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT21900.14799197194234395No Hit
GATTATTACTGCGGAACATGGGATAACAGCCTGAATATTTATGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTC21480.14515376974070995No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG21150.14292375372514038No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT21140.14285617748224436No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCT21130.14278860123934828No Hit
GTATCAGGGAGAACTGATTCTTGGACGTGTCTACTGATATGGAGACTCGA20750.14022070400929848No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGCCAGA20300.13717977307897636No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGA20130.13603097694974356No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20090.13576067197815936No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19650.1327873172907333No Hit
GTTTGGGGGCTGTTCCTGGGATCTGCTGGTACCAGGATACAAAATTACTC19500.13177367364729256No Hit
GGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAA19310.13048972503226766No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGG19160.12947608138882696No Hit
GGATAACAGCCTGAATATTTATGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGC19150.12940850514593089No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG18680.12623242172981666No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC18480.12488089687189571No Hit
GGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGGCCCAGT18100.12231299964184592No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT18040.12190754218446964No Hit
GGGTGGCTGACGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGAATCCCT17920.12109662726971705No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGAATCCCTGAG17880.12082632229813287No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCT17350.11724478142464236No Hit
GTCTATTACTGTGCGAGAGATATTGGATACAACTTTGGTTCAGGCTTTGG17320.11704205269595422No Hit
GTTCCTGGGATCTGCTGGTACCAGGATACAAAATTACTCCCAATGTTGGAGAAGGTTCCAGAGCAGGAGATGGT16800.11352808806535973No Hit
ACTCAGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGGC16760.11325778309377556No Hit
CCCTGATACTGCGCTCTGTGACCGCCGCAGACACGGCCGTCTATTACTGTGCGAGAGATATTGGATACAACTTTG16200.1094735134915969No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16080.10866259857684434No Hit
GCCCCCAAACTCCTCATTTATGACACTAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGT15980.10798683614788385No Hit
ACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAACATGGGATAACAGCCTGAATATTTATGTGGTGTTCG15550.1050810577033538No Hit
CTCCTGCTCTGGAACCTTCTCCAACATTGGGAGTAATTTTGTATCCTGGTACCAGCAGATCCCAGGAACAGCCCC15330.10359438035964076No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT15300.10339165163095261No Hit
CTAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAGCCACCCTGGGCATCA15250.10305377041647239No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC15110.10210770301592773No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG15080.10190497428723957No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14990.10129678810117515No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA14980.10122921185827911No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14920.10082375440090281No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACATAAATATTCA14840.10028314445773444No Hit
TGGGATAACAGCCTGAATATTTATGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAG14810.10008041572904629No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAG14810.10008041572904629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT859.609303E-783.9830170
ATAGCTA550.001456651377.8751570
AATGGTA5100.069.9858470
GGTATCA13750.044.5155871
GGTAGAA32300.043.75956770
CTAGTCA8300.043.0033570
AGTTGGT11650.042.89261270
CAGCCAT14250.041.07800770
TCTTGTT6150.039.46518770
AACCAAA9100.039.2228370
CGTGACT350.001484234438.66337632
TGACAGT33650.038.60972270
TCTATAC451.17304226E-437.3188443
GTATCAA34050.036.7425421
ATGGCAA3154.564572E-836.25932770
AGTGGAA24450.036.20371670
TATTCTA2001.5221331E-435.6927870
AAGCGGT14450.035.56927570
GTACGTA801.8912149E-434.4058969
GTGGTAT3950.034.0569951
Produced by FastQC (version 0.11.5)