FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004685294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965772
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTATATGTGTCGCCAGGGGAAAGAGTCACCCTCTCCTGCGGGGCCAGTCTGAGTGTTGATAAGAACTTAGC55920.5790186503646824No Hit
GTATAATAACTGGCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA48510.5022924665448988No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT41480.42950095881843753No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCCTCAGACTGCAGGCTATTGATGGTGAGAGTGAAC40770.42214932717038806No Hit
GTAATATACGGCCGTGTCTGCAGCGGTCACAGAGTTTAATTTGAGGGAGA37580.3891187568080251No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34070.35277477499865395No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCA27340.2830895905037628No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT24440.253061799265251No Hit
GTATATTACTGTGCAAGAAGATTTAACGGATACTATGATGGTGGTGGTTATTGGCAAGAGAAGTGGTTCTTCGA24340.25202635818806096No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22740.23545930095301998No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22380.23173171307513574No Hit
GAGTTCACTCTCACCATCAATAGCCTGCAGTCTGAGGATTTTGCAGTTTATTACTGTCAGCAGTATAATAAC21800.22572615482743338No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAATAGCCTGCAGTCTGAGGATTTTGCAGTTTATTACTGTCAGCAGTA21000.2174426262099129No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC20830.21568237637868978No Hit
CCACAAAGAGTGATGACGCAATCTCCAGCCACCCTATATGTGTCGCCAGG20410.21133352385449153No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20300.21019453866958246No Hit
GTCTGAGTGTTGATAAGAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGG19770.20470670096047516No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCC19460.20149683362118592No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19170.1984940544973348No Hit
CTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA18910.1958019076966406No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA18660.19321330500366546No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18080.1872077467559631No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17870.18503332049386398No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17810.18441205584754994No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17730.18358370298579788No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17550.18171990904685578No Hit
GGCCAAAAGTGTACGGCCAGTTATTATACTGCTGACAGTAATAAACTGCAAAATCCTCAGACTGCAGGCTATTGA16960.17561080669143442No Hit
CATATAGGGTGGCTGGAGATTGCGTCATCACTCTTTGTGGAGTGGAATCT16420.17001942487460808No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC16210.16784499861250896No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCACTCCACAAAGAGTGA16110.1668095575353189No Hit
CTCCTGCGGGGCCAGTCTGAGTGTTGATAAGAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGC16050.16618829288900486No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTCTTATCAACACTCAGAC15880.16442804305778175No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGTGGATGCACCATAGA15540.16090754339533553No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15270.1581118524869224No Hit
GGGGAAAGAGTCACCCTCTCCTGCGGGGCCAGTCTGAGTGTTGATAAGAACTTAGCCTGGTACCAGCAGAAAC14960.1549019851476332No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAAGTG14440.14951769154624486No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT14210.14713617706870774No Hit
CTATGATGGTGGTGGTTATTGGCAAGAGAAGTGGTTCTTCGATCTGTGGGGCCGTGGCTCCCTGGTCACTGTCTC13890.14382276562169952No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA13730.14216605989819542No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13310.1378172073739972No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAA13170.1363675898659311No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13140.13605695754277405No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12910.13367544306523693No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG12850.1330541784189229No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12850.1330541784189229No Hit
GTCCTGGACCTCCTGTACAAGAACATGAAACAGCTGTGGTTCTTCCTTCTCCTGGTGGCAACTCCCAGATGGGTC12360.12798051714069159No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12180.12611672320174946No Hit
GTAATAAACTGCAAAATCCTCAGACTGCAGGCTATTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA12140.12570254677087345No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12090.12518482623227842No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12030.12456356158596438No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11990.12414938515508836No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11600.12011116495404713No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11200.11596940064528688No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11130.11524459189125383No Hit
TCTATGGTGCATCCACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCAC11020.11410560670634477No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT10980.11369143027546874No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10940.11327725384459271No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA10940.11327725384459271No Hit
CCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAG10720.1109992834747746No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10520.10892840132039447No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10410.1077894161354854No Hit
GAGTCACCCTCTCCTGCGGGGCCAGTCTGAGTGTTGATAAGAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG10370.10737523970460938No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10320.10685751916601435No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA10290.10654688684285732No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG10270.10633979862741932No Hit
CTGCTACTCTGGCTCCCAGATTCCACTCCACAAAGAGTGATGACGCAATC10200.10561498987338627No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10170.10530435755022925No Hit
GGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGT9740.10085196091831197No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9670.10012715216427895No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)