FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965772
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTATATGTGTCGCCAGGGGAAAGAGTCACCCTCTCCTGCGGGGCCAGTCTGAGTGTTGATAAGAACTTAGC52930.5480589621566995No Hit
GTATAATAACTGGCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT44550.46128899988817235No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCCTCAGACTGCAGGCTATTGATGGTGAGAGTGAACT39930.4134516221219915No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39560.4096204901363883No Hit
GTAATATACGGCCGTGTCTGCAGCGGTCACAGAGTTTAATTTGAGGGAGA36900.38207775748313266No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA33900.3510145251674308No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCA27750.28733489892024205No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT23590.2442605501091355No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23190.24011878580037527No Hit
GTATATTACTGTGCAAGAAGATTTAACGGATACTATGATGGTGGTGGTTATTGGCAAGAGAAGTGGTTCTTC23040.23856562418459018No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21490.22251628748814423No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC20650.2138185824397477No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAATAGCCTGCAGTCTGAGGATT19600.20294645112925203No Hit
GAGTTCACTCTCACCATCAATAGCCTGCAGTCTGAGGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTG19460.20149683362118592No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC19330.20015076022083886No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA18520.19176368749559938No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTG18410.1906247023106903No Hit
GTCTGAGTGTTGATAAGAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGT18370.1902105258798143No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC17980.18617230567877305No Hit
CCACAAAGAGTGATGACGCAATCTCCAGCCACCCTATATGTGTCGCCAGGGGAAAGAGTCACCCTCTCCTGCGGG17460.18078801207738474No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA17420.18037383564650872No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16900.17498954204512038No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16830.17426473329108733No Hit
CTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCT16750.1734363804293353No Hit
GGCCAAAAGTGTACGGCCAGTTATTATACTGCTGACAGTAATAAACTGCAAAATCCTCAGACTGCAGGCTATT16640.17229739524442622No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16370.16950170433601305No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT16210.16784499861250896No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA15930.16494576359637678No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCACTCCACAAAGAGT15650.16204652858024463No Hit
GGGGAAAGAGTCACCCTCTCCTGCGGGGCCAGTCTGAGTGTTGATAAGAACTTAGCCTGGTACCAGCAGAAAC15400.15945792588726945No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA14690.15210629423922No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGTGGATGCACCATA14660.151795661916063No Hit
CTCCTGCGGGGCCAGTCTGAGTGTTGATAAGAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT14340.1484822504690548No Hit
CTATGATGGTGGTGGTTATTGGCAAGAGAAGTGGTTCTTCGATCTGTGGGGCCGTGGCTCCCTGGTCACTGTCTC14050.14547947134520364No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA14030.14527238312976562No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTCTTATCAACACTCAGACTG13850.1434085891908235No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAAGTG13710.14195897168275742No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAA13620.14102707471328638No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13620.14102707471328638No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13370.13843847202031123No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13240.13709239861996414No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13230.13698885451224513No Hit
CATATAGGGTGGCTGGAGATTGCGTCATCACTCTTTGTGGAGTGGAATCTGGGAGCCAGAGTAGCAGGAGGAAGA12980.13440025181926996No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12890.1334683548497989No Hit
GTCCTGGACCTCCTGTACAAGAACATGAAACAGCTGTGGTTCTTCCTTCTCCTGGTGGCAACTCCCAGATGGGTC12820.13274354609576589No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA12390.12829114946384862No Hit
GTAATAAACTGCAAAATCCTCAGACTGCAGGCTATTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA12340.12777342892525356No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC12090.12518482623227842No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG11770.12187141478527024No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11510.11917926798457608No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11240.1163835770761629No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10980.11369143027546874No Hit
CCATCATAGTATCCGTTAAATCTTCTTGCACAGTAATATACGGCCGTGTCTGCAGCGGTCACAGAGTTTAATTT10810.11193118044424565No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT10790.11172409222880762No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10410.1077894161354854No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10410.1077894161354854No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10320.10685751916601435No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10290.10654688684285732No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10230.10592562219654328No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10140.10499372522707222No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10070.1042689164730392No Hit
GAGTCACCCTCTCCTGCGGGGCCAGTCTGAGTGTTGATAAGAACTTAGCCTGGTACCAGCAGAAACCTGGCCAG10050.10406182825760117No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG10030.10385474004216315No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG9890.10240512253409707No Hit
CTGCTACTCTGGCTCCCAGATTCCACTCCACAAAGAGTGATGACGCAATCTCCAGCCACCCTATATGTGTCGCCA9890.10240512253409707No Hit
CCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCC9830.10178385788778303No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9720.10064487270287398No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)