FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047855
Sequences flagged as poor quality0
Sequence length19-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACGTCACTGAGGCTGCTATCCCA39500.3769605527482333No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG36720.35043016447886394No Hit
CACCTGCACTGGGAACAGCATCAATGTTGCCAACGAAGGAGTTGCTTGGCTGCAGCAGCACCAGGGTCACCCTCC36510.34842607040096196No Hit
ATCCCATGCTGAGCAGTAATAGTCACCTTCGTCCTCAGACTGGAGTCCAGTTATGGTCAGGGAGGCTGTGTTTC35610.33983709578138194No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35410.33792843475480866No Hit
GACTTGGGACAGACCGCCACACTCACCTGCACTGGGAACAGCATCAATGTTGCCAACGAAGGAGTTGCTTGGC35020.33420654575299064No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACGTCACTGAGG33910.32361347705550864No Hit
GTAATAGTCACCTTCGTCCTCAGACTGGAGTCCAGTTATGGTCAGGGAGG31250.2982282854020833No Hit
GCATGGGATAGCAGCCTCAGTGACGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG31080.296605923529496No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC27860.26587648100166533No Hit
CCCAAACTCCTGTCCGACAGGAGTGACAACCGGCCCTCAGGGATCTCAGA26490.252802152969638No Hit
GTAATAAGTGGCTGTGTCCACAGGGTCCATGTTGGTCATTGTAAGGACCACCTGGTTTTTGGAGGTGTCCTTGG24710.2358150698331353No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTT23340.22274074180110795No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23290.22226357654446466No Hit
GCATCAATGTTGCCAACGAAGGAGTTGCTTGGCTGCAGCAGCACCAGGGT22960.21911428585061865No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG22840.21796908923467467No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22440.214151767181528No Hit
GTCCAAGGACTTGGGACAGACCGCCACACTCACCTGCACTGGGAACAGCATCAATGTTGCCAACGAAGGAGT21670.20680342222922066No Hit
GACTATTACTGCTCAGCATGGGATAGCAGCCTCAGTGACGTGGTCTTCGG21260.2028906671247453No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG20990.2003139747388713No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20740.19792814845565465No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC20350.19420625945383665No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20300.1937290941971933No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19880.18972090604138933No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19710.18809854416880198No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19640.1874305128095013No Hit
GGATAGCAGCCTCAGTGACGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC18900.18036846701117998No Hit
CTACTGGAGTGGGTGTGGGCTGGATCCGTCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCAACCATTTAT18620.17769634157397732No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18450.17607397970139No Hit
GACCATAACTGGACTCCAGTCTGAGGACGAAGGTGACTATTACTGCTCAGCATGGGATAGCAGCCTCAGTGACG17980.17158862628894264No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGC17940.17120689408362796No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17180.1639539821826493No Hit
GTTATGGTCAGGGAGGCTGTGTTTCCTGACCTGCTTGCAGAGAATCTCTCTGAGATCCCTGAGGGCCGGTTGTCA16830.160613825386146No Hit
GCATAGTGGGAATCTGCACCATGCCCTGGGCTCTGCTCCTCCTGACCCTCCTCACTCACTCTGCAGTGTCAGTGG16690.15927776266754465No Hit
GCCCTGGGCTCTGCTCCTCCTGACCCTCCTCACTCACTCTGCAGTGTCAG16570.15813256605160064No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAC16170.15431524399845398No Hit
AGCCAGGGCTGACTCAGCCACCTTCGGTGTCCAAGGACTTGGGACAGACC16170.15431524399845398No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16150.15412437789579667No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC16130.1539335117931393No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG15940.15212028381789466No Hit
CTATTACTGCTCAGCATGGGATAGCAGCCTCAGTGACGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGG15600.14887556007272No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACGTCACTGAGGC15060.143722175300972No Hit
CTGCTATCCCATGCTGAGCAGTAATAGTCACCTTCGTCCTCAGACTGGAGTCCAGTTATGGTCAGGGAGGCTGTG14860.14181351427439867No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT14840.1416226481717413No Hit
AATCTGCACCATGCCCTGGGCTCTGCTCCTCCTGACCCTCCTCACTCACT14720.14047745155579733No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14290.13637383034866465No Hit
GTGGGAATCTGCACCATGCCCTGGGCTCTGCTCCTCCTGACCCTCCTCACTCACTCTGCAGTGTCAGTGGTCCAG14170.13522863373272065No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14140.13494233457873464No Hit
GCACCAGGGTCACCCTCCCAAACTCCTGTCCGACAGGAGTGACAACCGGCCCTCAGGGATCTCAGAGAGATTC14030.1338925710141193No Hit
TAACTGGACTCCAGTCTGAGGACGAAGGTGACTATTACTGCTCAGCATGGGATAGCAGCCTCAGTGACGTGGTCT13960.13322453965481865No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13870.13236564219286065No Hit
GTGCAGGTGAGTGTGGCGGTCTGTCCCAAGTCCTTGGACACCGAAGGTGGCTGAGTCAGCCCTGGCTGGACCA13870.13236564219286065No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13710.130838713371602No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13550.1293117845503433No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13320.127116824369784No Hit
GGGCTGACTCAGCCACCTTCGGTGTCCAAGGACTTGGGACAGACCGCCAC13190.12587619470251132No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC13160.12558989554852532No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAG13110.125112730291882No Hit
CTGCTCCTCCTGACCCTCCTCACTCACTCTGCAGTGTCAGTGGTCCAGCCAGGGCTGACTCAGCCACCTTCGGTG12810.12224973875202198No Hit
CCGCCACACTCACCTGCACTGGGAACAGCATCAATGTTGCCAACGAAGGA12740.12158170739272132No Hit
GGTCAGGAAACACAGCCTCCCTGACCATAACTGGACTCCAGTCTGAGGACGAAGGTGACTATTACTGCTCAGCA12600.12024564467411998No Hit
GTATCAACGCAGAGTACGGGAAACAGAGCTTCAGCAAGCATAGTGGGAATCTGCACCATGCCCTGGGCTCTGCTC12170.11614202346698732No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT11970.11423336244041399No Hit
CCTCACTCACTCTGCAGTGTCAGTGGTCCAGCCAGGGCTGACTCAGCCAC11830.11289729972181267No Hit
TCTCTGGAGAGCAGGCTCACCATCACCAAGGACACCTCCAAAAACCAGGTGGTCCTTACAATGACCAACATGGAC11810.11270643361915532No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11810.11270643361915532No Hit
CATTGATGCTGTTCCCAGTGCAGGTGAGTGTGGCGGTCTGTCCCAAGTCC11780.11242013446516932No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11690.11156123700321133No Hit
GCACTGGGAACAGCATCAATGTTGCCAACGAAGGAGTTGCTTGGCTGCAGCAGCACCAGGGTCACCCTCCCAAA11680.11146580395188264No Hit
GAATCTCTCTGAGATCCCTGAGGGCCGGTTGTCACTCCTGTCGGACAGGAGTTTGGGAGGGTGACCCTGGTGC11670.11137037090055397No Hit
GAGCAGTAATAGTCACCTTCGTCCTCAGACTGGAGTCCAGTTATGGTCAGGGAGGCTGTGTTTCCTGACCTGCT11620.11089320564391066No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTG11580.11051147343859599No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG11460.109366276822652No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11460.109366276822652No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11450.10927084377132332No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11320.10803021410405066No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT11300.10783934800139333No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC11280.10764848189873599No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11150.10640785223146332No Hit
GAATCTGCACCATGCCCTGGGCTCTGCTCCTCCTGACCCTCCTCACTCAC11130.10621698612880599No Hit
GCTTGGTCCCTCCGCCGAAGACCACGTCACTGAGGCTGCTATCCCATGCTGAGCAGTAATAGTCACCTTCGTCC10810.10316312848628867No Hit
GTCAGTGGTCCAGCCAGGGCTGACTCAGCCACCTTCGGTGTCCAAGGACTTGGGACAGACCGCCACACTCACC10700.10211336492167332No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC10610.10125446745971534No Hit
GGGCTCTGCTCCTCCTGACCCTCCTCACTCACTCTGCAGTGTCAGTGGTCCAGCCAGGGCTGACTCAGCCACCT10570.10087273525440066No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG10500.10020470389509999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)