FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004685297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047855
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCTGCACTGGGAACAGCATCAATGTTGCCAACGAAGGAGTTGCTTGGCTGCAGCAGCACCAGGGTCACCCTCC39650.37839204851816327No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACGTCACTGAGGCTGCTATCCCAT35800.34165032375662663No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35710.34079142629466863No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT35300.3368786711901933No Hit
GACTTGGGACAGACCGCCACACTCACCTGCACTGGGAACAGCATCAATGTTGCCAACGAAGGAGTTGCTTGGCT32500.3101574168181666No Hit
ATCCCATGCTGAGCAGTAATAGTCACCTTCGTCCTCAGACTGGAGTCCAGTTATGGTCAGGGAGGCTGTGTTTC31650.30204560745522996No Hit
GCATGGGATAGCAGCCTCAGTGACGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAG29110.27780561241774865No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACGTCACTGAGG28120.2683577403362106No Hit
GTAATAGTCACCTTCGTCCTCAGACTGGAGTCCAGTTATGGTCAGGGAGGCTGTGTTTCCTGACCTGCTTGCAGA27630.26368152082110596No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25290.241350186810198No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC24390.232761212190618No Hit
CCCAAACTCCTGTCCGACAGGAGTGACAACCGGCCCTCAGGGATCTCAGA23490.22417223757103796No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG23210.22150011213383533No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23030.2197823172099193No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22630.21596499515677264No Hit
GCATCAATGTTGCCAACGAAGGAGTTGCTTGGCTGCAGCAGCACCAGGGTCACCCTCCCAAACTCCTGTCCGACA22330.21310200361691262No Hit
GTAATAAGTGGCTGTGTCCACAGGGTCCATGTTGGTCATTGTAAGGACCACCTGGTTTTTGGAGGTGTCCTTGGT22190.2117659408983113No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT21850.20852121715313662No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA21700.20708972138320667No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG20670.197260117096354No Hit
GTCCAAGGACTTGGGACAGACCGCCACACTCACCTGCACTGGGAACAGCATCAATGTTGCCAACGAAGGAGTT20660.19716468404502532No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA20340.19411082640250799No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19190.18313602549971134No Hit
GACTATTACTGCTCAGCATGGGATAGCAGCCTCAGTGACGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTA19180.18304059244838267No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18740.17884153818992132No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGG18140.17311555511020132No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAC17740.16929823305705463No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG17280.16490831269593598No Hit
GTGGGAATCTGCACCATGCCCTGGGCTCTGCTCCTCCTGACCCTCCTCACTCACTCTGCAGTGTCAGTGGTCCA17180.1639539821826493No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTG17000.16223618725873332No Hit
GACCATAACTGGACTCCAGTCTGAGGACGAAGGTGACTATTACTGCTCAGCATGGGATAGCAGCCTCAGTGACGT16900.16128185674544665No Hit
GGATAGCAGCCTCAGTGACGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC16510.15755996774362865No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGG16480.15727366858964267No Hit
GCATAGTGGGAATCTGCACCATGCCCTGGGCTCTGCTCCTCCTGACCCTC16370.1562239050250273No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCT16060.15326548043383864No Hit
GTTATGGTCAGGGAGGCTGTGTTTCCTGACCTGCTTGCAGAGAATCTCTCTGAGATCCCTGAGGGCCGGTTGTCA15970.15240658297188064No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15840.15116595330460797No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA15700.14982989058600665No Hit
CTGCTCCTCCTGACCCTCCTCACTCACTCTGCAGTGTCAGTGGTCCAGCC15570.14858926091873398No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT15510.148016662610762No Hit
CTGCTATCCCATGCTGAGCAGTAATAGTCACCTTCGTCCTCAGACTGGAG15460.14753949735411864No Hit
AGCCAGGGCTGACTCAGCCACCTTCGGTGTCCAAGGACTTGGGACAGACCGCCACACTCACCTGCACTGGGAACA15410.14706233209747532No Hit
GCCCTGGGCTCTGCTCCTCCTGACCCTCCTCACTCACTCTGCAGTGTCAG15170.1447719388655873No Hit
CTACTGGAGTGGGTGTGGGCTGGATCCGTCAGCCCCCAGGAAAGGCCCTG15020.14334044309565733No Hit
CTATTACTGCTCAGCATGGGATAGCAGCCTCAGTGACGTGGTCTTCGGCG14720.14047745155579733No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACGTCACTGAGGCTGCTATCCCATGCTGAGCAGTAATA14320.13666012950265066No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG13850.13217477609020334No Hit
GCACCAGGGTCACCCTCCCAAACTCCTGTCCGACAGGAGTGACAACCGGCCCTCAGGGATCTCAGAGAGATTCT13730.13102957947425933No Hit
AATCTGCACCATGCCCTGGGCTCTGCTCCTCCTGACCCTCCTCACTCACTCTGCAGTGTCAGTGGTCCAGCCAGG13380.127689422677756No Hit
GTGCAGGTGAGTGTGGCGGTCTGTCCCAAGTCCTTGGACACCGAAGGTGGCTGAGTCAGCCCTGGCTGGACCA13230.126257926907826No Hit
GGGCTGACTCAGCCACCTTCGGTGTCCAAGGACTTGGGACAGACCGCCAC13140.12539902944586798No Hit
GGTCAGGAAACACAGCCTCCCTGACCATAACTGGACTCCAGTCTGAGGACGAAGGTGACTATTACTGCTCAGCAT13070.12473099808656732No Hit
TAACTGGACTCCAGTCTGAGGACGAAGGTGACTATTACTGCTCAGCATGG12910.12320406926530866No Hit
CCTCACTCACTCTGCAGTGTCAGTGGTCCAGCCAGGGCTGACTCAGCCACCTTCGGTGTCCAAGGACTTGGGACA12840.12253603790600799No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12750.12167714044405No Hit
TCCTGACCCTCCTCACTCACTCTGCAGTGTCAGTGGTCCAGCCAGGGCTGACTCAGCCACCTTCGGTGTCCAAG12630.12053194382810599No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG12620.12043651077677732No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT12490.11919588110950464No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12210.11652375567230198No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG12200.11642832262097332No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGAT12110.11556942515901533No Hit
GTATCAACGCAGAGTACGGGAAACAGAGCTTCAGCAAGCATAGTGGGAATCTGCACCATGCCCTGGGCTCTGCTC12020.11471052769705732No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACGTCACTGA11970.11423336244041399No Hit
CCGCCACACTCACCTGCACTGGGAACAGCATCAATGTTGCCAACGAAGGAGTTGCTTGGCTGCAGCAGCACCAGG11970.11423336244041399No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11930.11385163023509932No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11930.11385163023509932No Hit
GAGCAGTAATAGTCACCTTCGTCCTCAGACTGGAGTCCAGTTATGGTCAGGGAGGCTGTGTTTCCTGACCTGCTT11610.11079777259258199No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTG11590.11060690648992465No Hit
GGGCTCTGCTCCTCCTGACCCTCCTCACTCACTCTGCAGTGTCAGTGGTCCAGCCAGGGCTGACTCAGCCACCTT11380.10860281241202266No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAA11290.10774391495006466No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC11200.10688501748810665No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTAT11030.10526265561551931No Hit
GGGTCACCCTCCCAAACTCCTGTCCGACAGGAGTGACAACCGGCCCTCAGGGATCTCAGAGAGATTCTCTGCAA10960.10459462425621865No Hit
CATTGATGCTGTTCCCAGTGCAGGTGAGTGTGGCGGTCTGTCCCAAGTCCTTGGACACCGAAGGTGGCTGAGTCA10940.10440375815356133No Hit
TCTCTGGAGAGCAGGCTCACCATCACCAAGGACACCTCCAAAAACCAGGTGGTCCTTACAATGACCAACATGGAC10830.10335399458894598No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10830.10335399458894598No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC10780.10287682933230265No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC10720.10230423102433066No Hit
CTGCAGTGTCAGTGGTCCAGCCAGGGCTGACTCAGCCACCTTCGGTGTCCAAGGACTTGGGACAGACCGCCACA10600.10115903440838665No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)