FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1099053
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTATTAGTGACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTCTAAGGCA68690.6249926072718969No Hit
ATATAATGCTTATCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA51110.46503671797447443No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT48190.4384683905143792No Hit
GCATTATATTGTTGGCAGTAATAAGTTGCACAATCATCAGGCTGCAGACTGCTGATGGTGAGAGTGAATTCTGTC46930.42700397524050254No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46480.422909541214118No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT33260.3026241682612213No Hit
GTCTGCATCTGTGGGGGACACAGTCACCTTCACTTGCCGGGCCAGTCAGAGTATTAGTGACTGGTTGGCCTGGTA31870.28997691649083346No Hit
CTAATACTCTGACTGGCCCGGCAAGTGAAGGTGACTGTGTCCCCCACAGA30810.2803322496731277No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30090.27378115523091245No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29510.2685038847080168No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC28760.2616798279973759No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28530.2595871172727794No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA28260.2571304568569487No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27410.24939652591822234No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26950.24521110446902925No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26290.23920593456366526No Hit
GTATTAGTGACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTCTAAGGCATCTA26160.2380230980671542No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC25690.23374668919515254No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25180.22910633063191674No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG24640.2241930098002553No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24600.22382906010902115No Hit
CCTTCCACCCTGTCTGCATCTGTGGGGGACACAGTCACCTTCACTTGCCGGGCCAGTCAGAGTATTAGTGACTGG24250.22064450031072208No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT24010.21846080216331698No Hit
ACTTATTACTGCCAACAATATAATGCTTATCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA23820.2167320411299546No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC23250.21154575802986753No Hit
GAATTCACTCTCACCATCAGCAGTCTGCAGCCTGATGATTGTGCAACTTATTACTGCCAACAATATAATGCTTA23030.20954403472807953No Hit
GGCCAAAAGTGTACGGATAAGCATTATATTGTTGGCAGTAATAAGTTGCACAATCATCAGGCTGCAGACTGCTGA21130.19225642439445595No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20780.18907186459615688No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC20000.18197484561709035No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATG19700.179245222932834No Hit
CTTCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCCAGAACACGGTGTA19450.17697053736262036No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACA18870.17169326683972475No Hit
GTAATAAGTTGCACAATCATCAGGCTGCAGACTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG18730.17041944292040512No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGG18220.16577908435716932No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17440.15868206537810278No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17290.15731725403597463No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17180.15631639238508063No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16510.1502202350569081No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG16430.14949233567443973No Hit
CAATAATATTTGGCCGAGTCCTCGATTCTGAGGCTGTGCGATTGCAGATACACCGTGTTCTGGGAATTGTCTC16300.14830949917792866No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16300.14830949917792866No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA15920.1448519771112039No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC15760.1433961783462672No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15650.1423953166953732No Hit
GTATGTGAAGTGACCACTACCACTAATGGCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGC15630.14221334184975612No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15410.14021161854796813No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15380.13993865627954247No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG15220.13848285751460576No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGTCACTA15210.1383918700917972No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15200.13830088266898868No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15080.13720903359528613No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14760.1342974360654127No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14630.1331145995689016No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14580.13265966245485886No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCATTTTCTAAAGTAGATGCCTTAGAGA14370.13074892657587942No Hit
ACCTTGATGGGACCCCATTTTCTAAAGTAGATGCCTTAGAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTC14060.12792831646881453No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14000.12738239193196324No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT13540.12319697048277017No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13520.12301499563715308No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTCTAAGGCATCTACTTTAGAAAATGGGGTCCCA13410.12201413398625909No Hit
GTGTACGGATAAGCATTATATTGTTGGCAGTAATAAGTTGCACAATCATC13300.12101327233536509No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTGGGGGACACAGTCACCTTCACTTGCCGGGCCAGTCAGAGTATTAGTGA13230.12037636037570526No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT13220.12028537295289672No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGTCTGCAGCCTGATGATTG12960.11791969995987456No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT12860.1170098257317891No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12860.1170098257317891No Hit
CTCCTGTGCAGCCTCCGGATTCACTTTTATCAGCCATGCCGTGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG12630.11491711500719257No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12540.11409822820191565No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12370.11255144201417037No Hit
GCCCCTAACCTCCTGATCTCTAAGGCATCTACTTTAGAAAATGGGGTCCCATCAAGGTTCAGCGGCAGTGGA12230.11127761809485075No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12200.11100465582642512No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11920.10845700798778585No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11910.1083660205649773No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA11910.1083660205649773No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11900.10827503314216876No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG11880.10809305829655166No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11850.10782009602812603No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT11680.10627330984038076No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11650.10600034757195513No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT11630.10581837272633804No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC11600.1055454104579124No Hit
TCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG11590.10545442303510386No Hit
ACTTTAGAAAATGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAG11590.10545442303510386No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11530.1049084984982526No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC11530.1049084984982526No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11480.10445356138420986No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11440.10408961169297568No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGT11390.10363467457893294No Hit
GATAAGCATTATATTGTTGGCAGTAATAAGTTGCACAATCATCAGGCTGCAGACTGCTGATGGTGAGAGTGAAT11350.10327072488769877No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11330.10308875004208168No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11310.1029067751964646No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGA11270.1025428255052304No Hit
ATCATCAGGCTGCAGACTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGAC11190.10181492612276205No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11150.10145097643152787No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG11140.10135998900871931No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11120.10117801416310224No Hit
GTCAGAGTATTAGTGACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTCTAAGG11110.10108702674029368No Hit
GTCTGCAGCCTGATGATTGTGCAACTTATTACTGCCAACAATATAATGCTTATCCGTACACTTTTGGCCAGGGGA11070.10072307704905951No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCT11050.10054110220344241No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGG11010.10017715251220824No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)