FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1099053
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTATTAGTGACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTCTAAGGCAT61350.5582078389304247No Hit
ATATAATGCTTATCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT46410.4222726292544582No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46380.42199966698603253No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA43640.3970691131364911No Hit
GCATTATATTGTTGGCAGTAATAAGTTGCACAATCATCAGGCTGCAGACTGCTGATGGTGAGAGTGAATTCTGTC43550.39625022633121426No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36720.3341058165529779No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT33110.30125935691909306No Hit
GTCTGCATCTGTGGGGGACACAGTCACCTTCACTTGCCGGGCCAGTCAGA29370.2672300607886972No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29170.26541031233252627No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC28560.259860079541205No Hit
CTAATACTCTGACTGGCCCGGCAAGTGAAGGTGACTGTGTCCCCCACAGATGCAGACAGGGTGGAAGGAGACTGG27650.2515802240656274No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCT26310.23938790940928237No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26270.23902395971804818No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26230.23866001002681403No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC25990.2364763118794089No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC25940.2360213747653662No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA25180.22910633063191674No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC24820.22583078341080914No Hit
GTATTAGTGACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTCTAAGGCATCTA24110.21937067639140242No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23870.21718697824399733No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA23240.211454770607059No Hit
ACTTATTACTGCCAACAATATAATGCTTATCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA22350.20335688997709847No Hit
GAATTCACTCTCACCATCAGCAGTCTGCAGCCTGATGATTGTGCAACTTATTACTGCCAACAATATAATGCTTAT22310.2029929402858643No Hit
CCTTCCACCCTGTCTGCATCTGTGGGGGACACAGTCACCTTCACTTGCCG21680.19726073264892596No Hit
GGCCAAAAGTGTACGGATAAGCATTATATTGTTGGCAGTAATAAGTTGCACAATCATCAGGCTGCAGACTGCT20460.1861602670662834No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20080.1827027449995587No Hit
CTTCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCCAGAACACGGTGTAT19720.17942719777845106No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGT19420.17669757509419473No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19360.17615165055734344No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19280.17542375117487508No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT18870.17169326683972475No Hit
GTAATAAGTTGCACAATCATCAGGCTGCAGACTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCC18370.16714389569929747No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCATTTTCTAAAGTAGATGCCTTAGA18240.1659610592027864No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC18120.16486921012908384No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA17990.16368637363257277No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17070.15531553073418664No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT16490.150038260211291No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA15930.14494296453401245No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG15780.14357815319188427No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15570.14166741731290483No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15150.13784594555494592No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14920.1357532348303494No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC14780.13447941091102977No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14680.1335695366829443No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14420.13120386368992215No Hit
CAATAATATTTGGCCGAGTCCTCGATTCTGAGGCTGTGCGATTGCAGATACACCGTGTTCTGGGAATTGTCTCTG14120.1284742410056658No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG14110.12838325358285724No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT13800.12556264347579232No Hit
GTATGTGAAGTGACCACTACCACTAATGGCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCT13730.12492573151613254No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA13580.12356092017400434No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA13560.12337894532838725No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGAT13520.12301499563715308No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13500.12283302079153598No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13480.12265104594591889No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGTCACTA13420.12210512140906762No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC13300.12101327233536509No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13290.12092228491255655No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13140.11955747357042835No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTGGGGGACACAGTCACCTTCACTTGCCGGGCCAGTCAGAGTATTAGT13110.11928451130200272No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13060.11882957418796No Hit
ACCTTGATGGGACCCCATTTTCTAAAGTAGATGCCTTAGAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCT12900.11737377542302327No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12850.11691883830898056No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12800.11646390119493782No Hit
GTGTACGGATAAGCATTATATTGTTGGCAGTAATAAGTTGCACAATCATCAGGCTGCAGACTGCTGATGGTGAGA12660.1151900772756182No Hit
CTCCTGTGCAGCCTCCGGATTCACTTTTATCAGCCATGCCGTGAGCTGGGTCCGCCAGGCTCCAGGGAAGGG12290.11182354263170202No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC12230.11127761809485075No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12210.11109564324923366No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAG12170.11073169355799947No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGTCTGGGCACTGTGTGGCC11960.10882095767902003No Hit
ATCATCAGGCTGCAGACTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGAC11860.10791108345093459No Hit
CTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCATTTTCTAAAGTA11840.1077291086053175No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGTCTGCAGCCTGATGATTGT11750.10691022180004059No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11730.1067282469544235No Hit
GTCTGCAGCCTGATGATTGTGCAACTTATTACTGCCAACAATATAATGCTTATCCGTACACTTTTGGCCAGGG11660.10609133499476367No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCA11500.10463553622982694No Hit
GCCTGATGATTGTGCAACTTATTACTGCCAACAATATAATGCTTATCCGTACACTTTTGGCCAGGGGACCAA11460.10427158653859277No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11420.1039076368473586No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTCTAAGGCATCTACTTTAGAAAATGGGGTCCCAT11350.10327072488769877No Hit
ATGCTTATCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA11320.10299776261927314No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11200.1019059135455706No Hit
GCCCCTAACCTCCTGATCTCTAAGGCATCTACTTTAGAAAATGGGGTCCCATCAAGGTTCAGCGGCAGTG11200.1019059135455706No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11120.10117801416310224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAA304.1031853E-7201.7777370
TCGGTTT550.001155529882.5454470
CCGTTTA305.760223E-574.6586769
ATATGCG150.00255902267.572914
CACGCCA700.003008058864.85712470
TCCTAGT750.00395361360.53331870
CATTTAT800.005104625656.7499970
GTCGTAT300.005386898355.99400369
TATTCTA1401.3138733E-554.04760770
GTGGTAT1800.052.6045721
GCGAGTC200.007030053552.3517856
GTATCAA22450.040.3697851
CGTCACA12200.038.4535470
AATGGTA2458.191935E-637.06121470
ACATTTT3854.0345185E-935.37661470
CTGGTAT30500.035.22840570
GTGTCGG400.002863604833.8172261
TCAACGC27250.033.2285164
ATCAACG27350.033.1055153
AAGGACA25200.033.02909570
Produced by FastQC (version 0.11.5)