FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239345
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48230.3891571757662313No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT43920.35438074144003484No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGG38370.3095990220640742No Hit
GATCTATGCTGCATCCAGGTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA34530.27861491352286893No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT32010.258281592292703No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31380.25319826198516154No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30170.2434350402833755No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29870.24101440680359384No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29830.24069165567295628No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28380.22899192718734493No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27190.21939008105087768No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26750.2158398186138646No Hit
GCATAGATCAGGCGCTGAGGGGTTTTCCCTGGTTTCTGCTGATACCAGGCTAAATCATTTTTAATGCCCTGACT26620.21479087743929254No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT25680.20720622586930998No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC24260.19574856073167682No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24230.19550649738369866No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT23280.18784115803105675No Hit
GTAATACACGGCTGTGTCCTCGGTTGTCAGGCTGTTCATTTCCAGAAACA23010.18566258789925325No Hit
ACTTATTACTGTCTACAGCATAACTGTCAACAGCAAGATAGTTGCCGGAC21800.1758993661974672No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAAAAATGATTTAGCCTGGTATCAGCAGAAA21590.17420492276162006No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAAAAA21200.17105809923790388No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC21090.17017053362865062No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC20840.1681533390621659No Hit
CAATCAACACCCTGCAGCCTGATGATTTTGCAACTTATTACTGTCTACAGCATAACTGTCAACAGCAAGATAGT20490.16532926666908732No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19750.15935837075229253No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTCCGGCAACTA19620.15830942957772048No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19230.15516260605400434No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTG19210.15500123048868555No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19180.1547591671407074No Hit
GTGTTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTGCAACC18790.15161234361699125No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGGTGTTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG18570.1498372123984847No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18230.14709382778806546No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT18120.1462062621788122No Hit
GCATAACTGTCAACAGCAAGATAGTTGCCGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTG17920.14459250652562441No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17270.13934780065276417No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17210.13886367395680785No Hit
GGACTGAACACAGAGGACTCACCATGGAATTTGGGCTGAGCTGGATTTTC16280.1313597101694847No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTGTGACA16150.13031076899491265No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16050.12950389116831876No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16050.12950389116831876No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16000.1291004522550218No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15960.12877770112438425No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15890.12821288664576852No Hit
CCCTTAGAGTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTAACACCTGG15700.12667981877524015No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTCCGGCAACTATCTTGCTGTTGACAGT15620.12603431651396504No Hit
CAGTTATGCTGTAGACAGTAATAAGTTGCAAAATCATCAGGCTGCAGGGTGTTGATTGTGAGAGTGAATTCTGTC15600.12587294094864626No Hit
GAATTCACTCTCACAATCAACACCCTGCAGCCTGATGATTTTGCAACTTATTACTGTCTACAGCATAACTGTCAA15390.12417849751279911No Hit
GTACTGGGGCCAGGGAACCCTGGTCACCGTCTCTTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCC15230.12288749299024888No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15200.12264542964227072No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA15130.122080615163655No Hit
GTACTGGGGACCATAGGATTCTGTGACACAGTAATACACGGCTGTGTCCT15100.12183855181567683No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG14920.12038617172780784No Hit
GTCTACAGCATAACTGTCAACAGCAAGATAGTTGCCGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAA14750.11901447942259823No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT14720.11877241607462007No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14670.11836897716132311No Hit
GGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14580.11764278711738861No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14510.11707797263877291No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAAAAATGATTTAGCCTGGTA14290.11530284142026634No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14120.11393114911505675No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA13860.11183326676591264No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13850.11175257898325326No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG13820.11151051563527507No Hit
CAGTAATACACGGCTGTGTCCTCGGTTGTCAGGCTGTTCATTTCCAGAAACAATGTGTTTTTTGAATCATCTCT13800.1113491400699563No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT13530.1091705699381528No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTGTGACATCCAGATGACCCAGTCTCCATCCTCC13510.10900919437283403No Hit
GTGTATTACTGTGTCACAGAATCCTATGGTCCCCAGTACTGGGGCCAGGGAACCCTGGTCACCGTCTCTTCAGCA13430.10836369211155893No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13190.1064271853277336No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13060.10537824415316156No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC13030.10513618080518337No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12830.10352242515199561No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12770.10303829845603929No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG12720.10263485954274233No Hit
GCCTTGGGATTCCCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAGA12700.10247348397742356No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12660.102150732846786No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12550.10126316723753273No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC12430.10029491384562006No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGAT150.002552778267.615071
TCTTGAG11350.055.69381770
ACGGAGA550.005894947354.7294270
AATGGTA3350.044.92713570
TGTTAGA14200.043.1026370
CGCGTAT405.2747724E-542.73897639
CGTATGC5500.041.7516341
TCGTATG5500.041.0662940
CTCTTAT1650.040.978831
GTGGTAT2150.040.883531
ATCTCGT4950.039.96125837
TATACAC6550.039.730383
GTATCAA26900.038.583321
CACGAGA6500.038.4963521
CTTATAC6600.038.4176521
GTTATTA1050.001568737838.2237270
ACGAGAC6700.037.85495422
ATGCCGT6300.037.67508744
GTATGCC6500.037.48542
CATCTCG5300.037.2737436
Produced by FastQC (version 0.11.5)