FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239345
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66050.5329428044652619No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA45710.3688238545360654No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43790.3533318002654628No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG35230.28426305830902615No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT31200.2517458818972925No Hit
GATCTATGCTGCATCCAGGTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA31080.2507776285053798No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31050.25053556515740166No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC29440.23754483214924013No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA29200.23560832536541482No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27520.2220527778786375No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA25600.2065607236080349No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC24350.1964747507756113No Hit
GCATAGATCAGGCGCTGAGGGGTTTTCCCTGGTTTCTGCTGATACCAGGCTAAATCATTTTTAATGCCCTGACTT23840.19235967385998248No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23070.18614671459520957No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22700.1831612666368122No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC22400.1807406331570305No Hit
GTGTTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTGCAACCTG22030.17775518519863315No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21510.17355942050034492No Hit
ACTTATTACTGTCTACAGCATAACTGTCAACAGCAAGATAGTTGCCGGACGTTCGGCCAAGGGACCAAGGTGGAA20770.1675885245835502No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA20650.16662027119163753No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCA20440.16492582775579034No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTGT20120.16234381871068992No Hit
GTAATACACGGCTGTGTCCTCGGTTGTCAGGCTGTTCATTTCCAGAAACAATGTGTTTTTTGAATCATCTCTTGA19830.16000387301356767No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT19780.1596004341002707No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAAAAATGATTTAGCCTGGTATCAGCAGAAA19340.1560501716632576No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19100.1541136648794323No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT18860.15217715809560695No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGGTGTTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCC18670.15064409022507858No Hit
CAATCAACACCCTGCAGCCTGATGATTTTGCAACTTATTACTGTCTACAGCATAACTGTCAACAGCAAGATAGTT18390.1483848323106157No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG17810.14370494091637115No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17180.13862161060882966No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTCCGGCAACTAT16570.13369965586660695No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA16560.13361896808394758No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16150.13031076899491265No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16130.13014939342959386No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC16080.12974595451629692No Hit
GCATAACTGTCAACAGCAAGATAGTTGCCGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTG15780.12732532103651525No Hit
GGACTGAACACAGAGGACTCACCATGGAATTTGGGCTGAGCTGGATTTTC15380.12409780973013972No Hit
GAATTCACTCTCACAATCAACACCCTGCAGCCTGATGATTTTGCAACTTATTACTGTCTACAGCATAACTGTCAA15170.12240336629429253No Hit
CAGTTATGCTGTAGACAGTAATAAGTTGCAAAATCATCAGGCTGCAGGGTGTTGATTGTGAGAGTGAATTCTGTC15110.12191923959833623No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC15000.12103167398908295No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14970.12078961064110477No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTGTGACAT14790.11933723055323577No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14530.11723934820409168No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14110.11385046133239735No Hit
CCCTTAGAGTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTAACACCTGGATGAGCTGGGTCCGCCAGGCTCCAG14090.11368908576707858No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAG14020.11312427128846285No Hit
GTACTGGGGCCAGGGAACCCTGGTCACCGTCTCTTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCT13950.11255945680984714No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13840.11167189120059387No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG13740.11086501337399998No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13740.11086501337399998No Hit
GTACTGGGGACCATAGGATTCTGTGACACAGTAATACACGGCTGTGTCCTCGGTTGTCAGGCTGTTCATTTCCA13700.11054226224336242No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT13500.10892850659017464No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13490.10884781880751526No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGTCTGGGCACTGTGTGGCC13390.10804094098092137No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA13360.10779887763294321No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAACACCCTGCAGCCT13180.10634649754507422No Hit
GGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC13120.10586237084911787No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC13070.10545893193582094No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13050.10529755637050216No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGG12970.10465205410922707No Hit
GTCTACAGCATAACTGTCAACAGCAAGATAGTTGCCGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAA12940.10440999076124888No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12740.1027962351080611No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12640.10198935728146723No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA12630.10190866949880784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATTA1606.524715E-965.1548170
AATGGTA2800.058.50636370
TATTCTA1451.7788885E-551.3535570
CATTTAA1653.818211E-545.12887270
ATGGCAA2103.174333E-642.55008370
GTGGTAT2900.040.7193761
TAGGCAA2204.3705168E-640.6159970
GTATCAA28450.040.2020761
CCTGCGT2001.1869053E-437.23132370
GTCGTAA1200.001450112237.2313270
GAGCAAA28300.035.7841770
GACAGTT15850.035.70448370
CGCAATT601.1348133E-535.35722460
AAGGACA29150.034.7407270
GTAGATA2151.8160629E-434.63378570
TTAACGG601.4825644E-534.00403235
TATCAAC33800.033.4003222
CTCTTGA7900.032.96676669
ATCAACG34550.032.6726463
TCAACGC34500.032.6249584
Produced by FastQC (version 0.11.5)