FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960711
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCG42990.4474810843219241No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39220.4082393144244211No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT29600.3081051429618272No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29580.30789696381117737No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25110.26136892364092845No Hit
GTATGAGAATCTCCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA24320.25314584719025807No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24100.2508558765331093No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT22360.23274429042656947No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22260.23170339467332005No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA22180.23087067807072054No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTTCTCGATGGGGGCTGGGAGGCCTTTG21830.2272275429343476No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCT21690.2257702888797984No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21680.22566619930447346No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20550.21390407729275507No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19660.20464010508883523No Hit
GTAATACACGGCCGTGTCGTCAGATGTCAGCCTGGTCAGGTCCATGTAGACTGTATTGATGGACGTGTCCCTGG19420.20214195528103665No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAG19370.20162150740441193No Hit
GTCCCAACCAGGACACAGCTTGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGG18870.19641702863816488No Hit
ATATTATTGTCAACAGTATGAGAATCTCCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTG17870.1860080711056707No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16760.17445412824460216No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT15430.16061021472638495No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC15000.15613436298741243No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14950.15561391511078773No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGTGTGTAGTGGTTGTGCAG14450.15040943634454068No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC14350.14936854059129123No Hit
GTCTGAAGATATTGCAACATATTATTGTCAACAGTATGAGAATCTCCCTCCCACTTTCGGCGGAGGGACCAAGG14010.14582949503024323No Hit
CTATCTACACTGGGTGCGACAGGCCCCCGGACAAGGGCTTGAGTGGATGGGACGGATCAACCCTGACAGTGGTGA13730.14291498692114488No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT13500.14052092668867122No Hit
TTCTCATACTGTTGACAATAATATGTTGCAATATCTTCAGACTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTC13440.13989638923672154No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT13350.13895958305879708No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC13280.1382309560315225No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT13060.13594098537437377No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12920.1344837313198246No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12640.13156922321072623No Hit
CTACAAGACCACACCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG12220.12719746104707866No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12170.12667701317045396No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA11930.12417886336265538No Hit
GTGTATTACTGTGCGAGAAGTCTGACTACAATAAGTACTCGGTGGGGCCA11890.12376250506135561No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11720.12199298228083158No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGGAACTATTTAAATTGGTATCAACAGAAA11660.12136844482888196No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11470.11939074289770805No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11420.11887029502108334No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11270.11730895139120921No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11040.11491489115873556No Hit
CAATAATATGTTGCAATATCTTCAGACTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA11040.11491489115873556No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAACTTCGGCACCCAGAC10990.11439444328211086No Hit
GTATTGATGGACGTGTCCCTGGTCATGGTGACCCTGCCCTGAAACTTCTG10910.11356172667951131No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10900.11345763710418638No Hit
GTCTCCATCCTCCCTGTCTGCAGCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGGAA10840.11283309965223673No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10780.11220856220028708No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCT10710.1114799351730125No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10620.11054312899508802No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT10570.1100226811184633No Hit
GCCTCATGCATCACGGAGCATGAGAAGACGTTCCCCTGCTGCCACCTGCT10410.10835724791326423No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG10400.1082531583379393No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG10370.10794088961196446No Hit
GTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC10350.10773271046131459No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10250.10669181470806517No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10180.10596318768079059No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10160.10575500853014069No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10060.10471411277689127No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT9980.10388139617429175No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9940.10346503787299198No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9920.1032568587223421No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9900.10304867957169223No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9830.10232005254441762No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGGGAGGGAGAT9790.10190369424311785No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTAT200.00660534953.18265562
ACCGTAA200.007839547550.9279527
GATTAAC200.00793055350.779481
GACCGTA1700.047.8971826
TTCGGGG6500.047.61045570
ACCGTAC1650.045.26928727
TGTTAGA11850.043.80654570
CGTATGC1650.043.72294641
TCGTATG1650.043.67218840
CGTACTA1750.042.72932429
GCGTAGA20700.042.43911470
ATCTCGT1500.041.02725637
GTACTAG1850.040.4259730
CTAGATC1600.038.32402433
GTGGTAT2400.038.0846061
ATCTCCG2050.037.97546810
GCCGTCT2000.037.96360446
CAGTTCG10550.037.84961770
CCCACGA2150.037.8090619
CTTATAC2350.037.454371
Produced by FastQC (version 0.11.5)