FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960711
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTG37140.3865886827568332No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA36280.37763697927888823No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28210.29363669199166037No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27990.2913467213345116No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA24810.2582462363811802No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT24250.25241722016298346No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23760.24731683097206134No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC23520.2448186811642627No Hit
GTATGAGAATCTCCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT22810.23742832131619188No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22160.23066249892007068No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT22030.2293093344408464No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA20980.21837992903172757No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA20640.2148408834706795No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18830.19600067033686508No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18360.19110846029659284No Hit
GTAATACACGGCCGTGTCGTCAGATGTCAGCCTGGTCAGGTCCATGTAGACTGTATTGATGGACGTGTCCCTGGT18280.1902757436939933No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA18230.18975529581736858No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCA17810.18538353365372104No Hit
GTCCCAACCAGGACACAGCTTGGACATGAGGGTCCCTGCTCAGCTCCTGG17550.18267720469527257No Hit
ATATTATTGTCAACAGTATGAGAATCTCCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGT16550.17226824716277842No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15380.16008976684976023No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14760.15363621317961385No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14640.15238713827571454No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC14610.15207486954973973No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT14340.1492644510159663No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC13950.14520495757829358No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGTGTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGA13820.14385179309906934No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC13610.14166591201724554No Hit
GTCTGAAGATATTGCAACATATTATTGTCAACAGTATGAGAATCTCCCTCCCACTTTCGGCGGAGGGACCAAGGT13410.13958412051074673No Hit
CTATCTACACTGGGTGCGACAGGCCCCCGGACAAGGGCTTGAGTGGATGGGACGGATCAACCCTGACAGTGGT12890.13417146259384977No Hit
GTGTATTACTGTGCGAGAAGTCTGACTACAATAAGTACTCGGTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCA12750.13271420853930058No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT12670.13188149193670104No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12650.13167331278605118No Hit
TTCTCATACTGTTGACAATAATATGTTGCAATATCTTCAGACTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTC12540.1305283274574768No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12390.12896698382760266No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGG12350.12855062552630292No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC12300.1280301776496782No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGGAACTATTTAAATTGGTATCAACAGAAA11940.1242829529379803No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11750.12230525100680642No Hit
CTACAAGACCACACCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGA11650.121264355253557No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11600.12074390737693229No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA11530.12001528034965771No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTT11500.11970301162368287No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT11230.11689259308990944No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT11020.11470671200808569No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA10940.11387399540548615No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10840.11283309965223673No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10780.11220856220028708No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10720.11158402474833744No Hit
GTATTGATGGACGTGTCCCTGGTCATGGTGACCCTGCCCTGAAACTTCTGTGCATACTTTGTGTCACCACTGTCA10590.11023086026911318No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG10410.10835724791326423No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10360.10783680003663954No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10280.10700408343403998No Hit
GCCTCATGCATCACGGAGCATGAGAAGACGTTCCCCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTGTA10260.10679590428339011No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAACTTCGGCACCCAGAC10250.10669181470806517No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGGGAGGGAGATT10220.10637954598209036No Hit
GTCTCCATCCTCCCTGTCTGCAGCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGGAA10150.10565091895481575No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA10040.1045059336262414No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAACTGCTCCTCCCGTGGCTTTGTCTT9780.1017996046677929No Hit
CAATAATATGTTGCAATATCTTCAGACTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA9690.10086279848986843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA2750.071.22751670
CCTTTGT10200.069.9555970
GTAGTTT1504.797821E-755.96447870
GTGGTAT2700.051.3516041
CAATCTT850.00884798849.3804270
TAGGCAA1902.4482251E-644.1824870
AAACACA19450.035.96688570
ATCAACG33100.034.1881683
TCAACGC33100.033.985834
AACGCAG33550.033.8373376
CAACGCA33700.033.484475
GGCCTTT12000.032.64594370
ACGCAGA35250.032.2054677
GAGCAAA24450.032.04509770
AAAGTCA3501.373246E-731.979770
CGCAGAG35950.031.6723638
AGAGTAC36650.031.1644911
AATGCCA10350.031.09137370
GAGGGTT2253.4194943E-431.09137370
GCAGAGT37000.030.9561849
Produced by FastQC (version 0.11.5)