FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195545
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCCCACCGAGGAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTG41060.3434416939554764No Hit
CCCTAAGGCTGACCTCTGTGACCGCCGCGGACACGGCCATGTATTACTGTGCGAGGAATCTTTATATTTGGGA28530.23863593591207355No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT27680.23152620771280044No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCGCAGACCCACTGCCA26360.2204852180386351No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26250.21956513556578797No Hit
GTAATACATGGCCGTGTCCGCGGCGGTCACAGAGGTCAGCCTTAGGGAGA24100.20158170541468537No Hit
GCCTTAGGGAGATCAGCATCTTGGACTTGTCTATTGAGATGGTGATTCGACTGTTGAGGGACGGATTGTAATTGG22210.1857730156539486No Hit
CTCCTGTAGGGCCAGTCAGGATATAAGTAGCAGTACTTTAGCCTGGTACC20480.1713026276718986No Hit
ATGTATTACTGTGCGAGGAATCTTTATATTTGGGACTGTTTTGGTCTCGATTGTTATGGAGGGGGCGACTGGGGC17970.15030801851875086No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT17840.1492206483235679No Hit
GTGTATTACTGTCAGCAGTATGGTAGCCCACCGAGGAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA17710.14813327812838495No Hit
GTATGGTAGCCCACCGAGGAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA17540.14671133248853033No Hit
GTATTACTGTGCGAGGAATCTTTATATTTGGGACTGTTTTGGTCTCGATT17430.14579125001568322No Hit
CAGTAATACATGGCCGTGTCCGCGGCGGTCACAGAGGTCAGCCTTAGGGAGATCAGCATCTTGGACTTGTCTAT17300.1447038798205003No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16770.14027075517860055No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16660.13935067270575344No Hit
GGATATAAGTAGCAGTACTTTAGCCTGGTACCAGCACAAACCTGGCCAGC15980.13366289014633492No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15590.13040077956078608No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15510.12973162867144272No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15400.12881154619859564No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC15240.12747324441990893No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14100.11793784424676612No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14030.11735233721859067No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCGCAGACCCACTGCCA13810.11551217227289647No Hit
GTCTTGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC13710.11467573366121728No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13480.11275192485435512No Hit
GATAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACAA13240.11074447218632505No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT13240.11074447218632505No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13110.10965710199114212No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGGATATAAGTAG12900.1079005809066158No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12720.10639499140559328No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12670.10597677209975367No Hit
CATTTATGGTGTATCCAGAAGGGCCGCAGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGCGACAGACTTCA12470.10430389487639528No Hit
ATCCAGAAGGGCCGCAGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGCGACAGACTTCACTCTCACCATCAG12460.10422025101522736No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12340.10321652468121233No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCT12170.10179457904135772No Hit
GTTATGGAGGGGGCGACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCT12110.1012927158743502No Hit
ACTTTAGCCTGGTACCAGCACAAACCTGGCCAGCCTCCCAGGCTCCTCAT12010.100456277262671No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)