FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195545
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCCCACCGAGGAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGT31200.26096884684390803No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29290.24499286936083545No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28960.2422326219422941No Hit
CCCTAAGGCTGACCTCTGTGACCGCCGCGGACACGGCCATGTATTACTGTGCGAGGAATCTTTATATTTGG28230.236126620077036No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA25380.21228811964417899No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCGCAGACCCACTGCCA24840.20777135114111137No Hit
GTAATACATGGCCGTGTCCGCGGCGGTCACAGAGGTCAGCCTTAGGGAGATCAGCATCTTGGACTTGTCTATTGA22070.18460200159759776No Hit
GCCTTAGGGAGATCAGCATCTTGGACTTGTCTATTGAGATGGTGATTCGA20950.1752338891467908No Hit
CTCCTGTAGGGCCAGTCAGGATATAAGTAGCAGTACTTTAGCCTGGTACCAGCACAAACCTGGCCAGCCTCCCAG20500.17146991539423442No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT18540.15507571860532227No Hit
GTATTACTGTGCGAGGAATCTTTATATTTGGGACTGTTTTGGTCTCGATTGTTATGGAGGGGGCGACTGGGGCCA17790.1488024290177283No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17060.14269642715247022No Hit
ATGTATTACTGTGCGAGGAATCTTTATATTTGGGACTGTTTTGGTCTCGATTGTTATGGAGGGGGCGACTGGGGC16480.13784508320473088No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC16060.13433204103567828No Hit
CAGTAATACATGGCCGTGTCCGCGGCGGTCACAGAGGTCAGCCTTAGGGAGATCAGCATCTTGGACTTGTCTATT15760.13182272520064073No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15560.13014984797728232No Hit
GGATATAAGTAGCAGTACTTTAGCCTGGTACCAGCACAAACCTGGCCAGC15370.12856061461509186No Hit
GTGTATTACTGTCAGCAGTATGGTAGCCCACCGAGGAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA15090.12621858650239012No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC14710.12304011977800919No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG13980.1169341179127511No Hit
GTCTTGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC13620.113922938910706No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA13430.11233370554851554No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCGCAGACCCACTGCCA13250.11082811604749299No Hit
GTTATGGAGGGGGCGACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCA13220.11057718446398923No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13210.11049354060282131No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC13100.1095734581299742No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC12910.10798422476778373No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12430.1039693194317236No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA12350.10330016854238025No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGGATATAAGTA12320.1030492369588765No Hit
GATAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACAA12280.10271466151420483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTGGT10000.062.5986270
TATTCTA3100.058.3356370
AATGGTA4050.051.521570
TAGGCAA5250.042.39483370
GTGGTAT3650.039.9185181
GTATCAA25700.038.235231
GCTAAAT1500.001816542437.09547870
GGTATCA10150.037.0557631
GAGCCAT11650.037.01587770
TGGTATC3950.035.9927252
ACTGTTA1700.003356211132.73130470
GAGCAAA13550.031.82545770
GTAGTTT2203.1021412E-431.61546570
TCGCACA7050.031.57062170
TATCAAC30950.031.1707152
CGGCAAA2702.8374772E-530.91289970
AGTTTGT1800.004439924430.91289770
ATCAACG31450.030.7763523
AACGCAG32350.030.0272586
GTCGGAA2803.6372334E-529.80886870
Produced by FastQC (version 0.11.5)