FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308972
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG42330.323383540671611No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACAGTCGATCACGACTAGTGTCCCACACCTGA39110.29878408399874096No Hit
GGTGTATACTATTGTGCGAGAGACTCCATCCAGGTCTCCAGTGGGAGGTGTCTTGACTACTGGGGCCAGGGAACC38810.29649220915344254No Hit
CCTATGTTCTGACTCAGCCACTCTCGGTGTCAGTGGCCCCAGGACAGACG37100.2834285225352414No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACAGTCGATCACGACTAGTGTCCCACACCTGACAATAATAG34930.26685062782091595No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34840.2661630653673264No Hit
CCATTACCTGTGGAGGAGACAACATTGGAACTAAACCCGTCCACTGGTACCAACACAAGGCAGGCCAGGCCCCTA31360.23957731716186442No Hit
ATAGTATACACCCGTGTCCTCGGGCCTCAGACTGTTCATTTGCAAATACACCGTGTTCTTGGAATCGTCTC31120.23774381728562566No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG29850.22804154710719557No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC29820.22781235962266574No Hit
GTATACACCCGTGTCCTCGGGCCTCAGACTGTTCATTTGCAAATACACCG28390.21688775619340978No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA27310.20863700675033536No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT27190.20772025681221598No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT25900.19786519497743266No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT24910.19030200798794777No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTG24570.1877045498299429No Hit
CCCCAGGACAGACGGCCACCATTACCTGTGGAGGAGACAACATTGGAACT23710.181134508606754No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23450.179148217074162No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23370.1785370504487491No Hit
GCACTGAACACAGAGGACTCACTATGGAGTTTGGGCTGAGCTGGGTTTTC21950.16768884284766977No Hit
GTGTTGGTACCAGTGGACGGGTTTAGTTCCAATGTTGTCTCCTCCACAGGTAATGGTGGCCGTCTGTCCTGGGGC21830.1667720929095504No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG21590.16493859303331163No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG21110.16127159328083412No Hit
ACACAAGGCAGGCCAGGCCCCTATTTTGGTCGTCTATGATGATAGTGACCGGCCCTCAGGGATTCCTGACCGAT21050.16081321831177442No Hit
CTGCACAGACTCTGCGACCTCCTATGTTCTGACTCAGCCACTCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAC20930.15989646837365504No Hit
GGGCTGGAGTGGGTGTCCTTTATCGGGCATGATGGAAGTCGTGAAGAGTT20840.1592089059200655No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGGCATAGACGC20810.15897971843553568No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20710.1582157601537695No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20550.15699342690294368No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC20460.15630586444935415No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20360.155541906167588No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGCGACCTCCTATGTTCTGAC20120.15370840629134924No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20080.15340282297864277No Hit
GTATACTATTGTGCGAGAGACTCCATCCAGGTCTCCAGTGGGAGGTGTCTTGACTACTGGGGCCAGGGAACCC19980.15263886469687662No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19920.15218048972781695No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG19680.15034698985157818No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA19420.14836069831898618No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19370.14797871917810312No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19110.14599242764551115No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18940.14469369856650868No Hit
CAATAGTATACACCCGTGTCCTCGGGCCTCAGACTGTTCATTTGCAAATACACCGTGTTCTTGGAATCGTCTC18910.14446451108197883No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18890.14431171942562562No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18830.14385334445656592No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGC18310.13988076139138195No Hit
GTTTACAGACTCCGTGAAGGGCCGATTCACCGTGTCCAGAGACGATTCCAAGAACACGGTGTATTTGCAAATGAA18160.1387348239687327No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT17890.13667213660796412No Hit
GTCTGAGGCCCGAGGACACGGGTGTATACTATTGTGCGAGAGACTCCATC17880.1365957407797875No Hit
GTACCAACACAAGGCAGGCCAGGCCCCTATTTTGGTCGTCTATGATGATAGTGACCGGCCCTCAGGGATTCCTGA17760.13567899084166812No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT17550.1340746784499592No Hit
CTATCATCATAGACGACCAAAATAGGGGCCTGGCCTGCCTTGTGTTGGTACCAGTGGACGGGTTTAGTTCCAATG17550.1340746784499592No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG17460.13338711599636968No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17370.13269955354278012No Hit
CGGCCACCCTGACCATCACCAGGGTCGAAGCCGGGGATGAGGCCGACTAT17260.1318591994328374No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17210.1314772202919543No Hit
GGGTGGCCGTGTTCAGAGAGTTGGAGCCAGAGAATCGGTCAGGAATCCCTGAGGGCCGGTCACTATCATCATAGA17010.129949303728422No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG16910.12918534544665586No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG16830.12857417882124295No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC16810.12842138716488968No Hit
CCTTGGGGCTGGTCGGGGATGCTGACGAAACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGACACCTCCCA16560.12651149146047433No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16490.12597672066323803No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16430.12551834569417833No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG16320.12467799158423556No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC16270.12429601244335249No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG16250.12414322078699927No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC15950.12185134594170081No Hit
GTTTGGTCCCTCCGCCGAACAGTCGATCACGACTAGTGTCCCACACCTGACAATAATAGTCGGCCTCATCCCCGG15930.12169855428534758No Hit
GCCTTGTGTTGGTACCAGTGGACGGGTTTAGTTCCAATGTTGTCTCCTCCACAGGTAATGGTGGCCGTCTGTC15700.11994145023728545No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15690.11986505440910883No Hit
GTCTGTAAACTCTTCACGACTTCCATCATGCCCGATAAAGGACACCCACT15560.11887190864281283No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGT15410.11772597122016362No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG15360.11734399207928053No Hit
GATCAGCACTGAACACAGAGGACTCACTATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGG15320.11703840876657408No Hit
CCTCTCTCACTGCACAGACTCTGCGACCTCCTATGTTCTGACTCAGCCAC15230.11635084631298453No Hit
ATACTATTGTGCGAGAGACTCCATCCAGGTCTCCAGTGGGAGGTGTCTTGACTACTGGGGCCAGGGAACCCTGG15100.11535770054668855No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT15000.11459374226492239No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14960.11428815895221593No Hit
CTATTATTGTCAGGTGTGGGACACTAGTCGTGATCGACTGTTCGGCGGAG14930.1140589714676861No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14910.11390617981133287No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCGAGCACTAGAAG14810.11314222152956671No Hit
GACCATCACCAGGGTCGAAGCCGGGGATGAGGCCGACTATTATTGTCAGGTGTGGGACACTAGTCGTGATCGA14710.11237826324780055No Hit
GCCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGTCCTTTATCGGGCATGATGGAAGTCG14610.11161430496603442No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14570.11130872165332796No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14480.11062115919973842No Hit
GTCCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCTTCGACCCTGG14430.11023918005885534No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC14420.11016278423067873No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGCGACCTCCTATGTTCTGACTCAGCCACTCTCGGTG14370.10978080508979567No Hit
GTCTGGGGGAGGCGTTGTCCCGCCTGGGGCGTCCCTGAGACTCTCTTGTAAAACGTCTGGATTTCCCTTCAGCG14360.10970440926161905No Hit
AGGCAGGCCAGGCCCCTATTTTGGTCGTCTATGATGATAGTGACCGGCCCTCAGGGATTCCTGACCGATTCTC14360.10970440926161905No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC14360.10970440926161905No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACAGTCGA14360.10970440926161905No Hit
GGGTTCCCTGGCCCCAGTAGTCAAGACACCTCCCACTGGAGACCTGGATGGAGTCTCTCGCACAATAGTATACAC14320.10939882594891259No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAG14290.10916963846438273No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14200.10848207601079321No Hit
GAGTTTACAGACTCCGTGAAGGGCCGATTCACCGTGTCCAGAGACGATTC14130.10794730521355689No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACAGTCGATCACGA14060.1074125344163206No Hit
GGTTTAGTTCCAATGTTGTCTCCTCCACAGGTAATGGTGGCCGTCTGTCCTGGGGCCACTGACACCGAGAG13760.10512065957102215No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGCGA13730.1048914720864923No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT13670.10443309711743261No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT13670.10443309711743261No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACAGTCGATCACGACTAG13580.10374553466384306No Hit
GAGTACGGGAGCTCTGGGAGAGGAGCCGAGCACTAGAAGTCGGCGGTGTTTCCATTCGGTGATCAGCACTGAACA13520.10328715969478339No Hit
CCCTGAGACTCTCTTGTAAAACGTCTGGATTTCCCTTCAGCGTCTATGCC13400.10237040975666402No Hit
CTATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCCAGTCTCAGGTGCAATTGG13270.10137726399036802No Hit
CAATAATAGTCGGCCTCATCCCCGGCTTCGACCCTGGTGATGGTCAGGGT13230.10107168067766156No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGACGAAACGGTGACCAGGGTTCCC13200.1008424931931317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2900.095.9564370
ACCCGAA150.002414056668.5748741
CAACGCT200.007220790751.9991654
CCCCGTA650.00884067349.39769470
AATGGTA3250.046.10451570
ATCTCGT2350.045.120937
TCTCGTA2450.044.7010638
GCTAATC2550.044.17487733
CGTATGC2650.043.99142541
TCGTATG2750.043.60836440
CTTATAC3050.043.5647931
TTATACA3100.042.839212
GGTATCA9350.041.175391
GTGGTAT2900.041.1188471
TACACAT3250.040.862025
CGAGACT3100.040.64698823
AAAGGGG2500.040.20018468
TGGTATC3050.040.192152
ACGAGAC3200.039.3737622
GCTTAGT350.001433613638.9370271
Produced by FastQC (version 0.11.5)