FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308972
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT40700.3109310206788228No Hit
GGTGTATACTATTGTGCGAGAGACTCCATCCAGGTCTCCAGTGGGAGGTGTCTTGACTACTGGGGCCAGGGAACC39750.30367341700204437No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACAGTCGATCACGACTAGTGTCCCACACCT37010.28274096008165184No Hit
CCTATGTTCTGACTCAGCCACTCTCGGTGTCAGTGGCCCCAGGACAGACG35720.27288589824686854No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34750.2654755029137369No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33550.2563080035325431No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29280.2236869849011285No Hit
CCATTACCTGTGGAGGAGACAACATTGGAACTAAACCCGTCCACTGGTAC29050.22192988085306636No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACAGTCGATCACGACTAGTGTCCCACACCTGACAATAATAGT28780.21986719349229777No Hit
ATAGTATACACCCGTGTCCTCGGGCCTCAGACTGTTCATTTGCAAATACACCGTGTTCTTGGAATCGTCTCTG28010.21398471472269842No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC26330.20115021558902713No Hit
CTGCACAGACTCTGCGACCTCCTATGTTCTGACTCAGCCACTCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAC23530.17975938369957492No Hit
GTATACACCCGTGTCCTCGGGCCTCAGACTGTTCATTTGCAAATACACCGTGTTCTTGGAATCGTCTCTGGACAC23460.17922461290233863No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23380.1786134462769257No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG23120.17662715474433371No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23030.17593959229074418No Hit
GGGCTGGAGTGGGTGTCCTTTATCGGGCATGATGGAAGTCGTGAAGAGTT22710.1734949257890925No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG22590.17257817585097313No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC22530.17211980088191345No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22280.17020990517749807No Hit
GTCTGAGGCCCGAGGACACGGGTGTATACTATTGTGCGAGAGACTCCATCCAGGTCTCCAGTGGGAGGTGTCTT22060.16852919695761254No Hit
CCCCAGGACAGACGGCCACCATTACCTGTGGAGGAGACAACATTGGAACT21860.16700128039408024No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21840.166848488737727No Hit
GGGTGGCCGTGTTCAGAGAGTTGGAGCCAGAGAATCGGTCAGGAATCCCTGAGGGCCGGTCACTATCATCATA21720.16593173879960763No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTG21630.1652441763460181No Hit
GTACCAACACAAGGCAGGCCAGGCCCCTATTTTGGTCGTCTATGATGATAGTGACCGGCCCTCAGGGATTCCT21160.1616535724217172No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGGCATAGACGC20960.1601256558581849No Hit
GCACTGAACACAGAGGACTCACTATGGAGTTTGGGCTGAGCTGGGTTTTC20960.1601256558581849No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA20910.15974367671730183No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGCGACCTCCTATGTTCTGACT20880.15951448923277198No Hit
GTGTTGGTACCAGTGGACGGGTTTAGTTCCAATGTTGTCTCCTCCACAGGTAATGGTGGCCGTCTGTCCTGGGGC20880.15951448923277198No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC19910.15210409389964033No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGC19880.15187490641511048No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19850.15164571893058065No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA19840.151569323102404No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19570.14950663574163542No Hit
ACACAAGGCAGGCCAGGCCCCTATTTTGGTCGTCTATGATGATAGTGACCGGCCCTCAGGGATTCCTGACCGATT19550.14935384408528218No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19200.14667999009910065No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG19090.1458396359891579No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18900.14438811525380224No Hit
CCTCTCTCACTGCACAGACTCTGCGACCTCCTATGTTCTGACTCAGCCACTCTCGGTGTCAGTGGCCCCAGGACA18680.14270740703391668No Hit
GTATACTATTGTGCGAGAGACTCCATCCAGGTCTCCAGTGGGAGGTGTCTTGACTACTGGGGCCAGGGAACCCTG18480.14117949047038442No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18230.13926959476596903No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCG18090.13820005317149642No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG17920.13690132409249395No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17900.1367485324361407No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG17550.1340746784499592No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG17470.1334635118245463No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17260.1318591994328374No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA17150.1310188453228946No Hit
CAATAGTATACACCCGTGTCCTCGGGCCTCAGACTGTTCATTTGCAAATACACCGTGTTCTTGGAATCGTCTCTG17110.13071326201018815No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG16990.12979651207206877No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16900.12910894961847924No Hit
GTTTACAGACTCCGTGAAGGGCCGATTCACCGTGTCCAGAGACGATTCCAAGAACACGGTGTATTTGCAAATGAA16710.12765742888312356No Hit
GCCTTGTGTTGGTACCAGTGGACGGGTTTAGTTCCAATGTTGTCTCCTCCACAGGTAATGGTGGCCGTCTGTC16330.12475438741241218No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTG16260.12421961661517587No Hit
CTATCATCATAGACGACCAAAATAGGGGCCTGGCCTGCCTTGTGTTGGTA16010.12230972091076049No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT15990.12215692925440727No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCT15990.12215692925440727No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGCGACCTCCTA15820.12085820017540483No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG15810.1207818043472282No Hit
CTATTATTGTCAGGTGTGGGACACTAGTCGTGATCGACTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCA15740.1202470335499919No Hit
GTTTGGTCCCTCCGCCGAACAGTCGATCACGACTAGTGTCCCACACCTGACAATAATAGTCGGCCTCATCCCCG15410.11772597122016362No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT15400.11764957539198699No Hit
CGGCCACCCTGACCATCACCAGGGTCGAAGCCGGGGATGAGGCCGACTATTATTGTCAGGTGTGGGACACTAGTC15360.11734399207928053No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT15210.1161980546566313No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAG15000.11459374226492239No Hit
CCTTGGGGCTGGTCGGGGATGCTGACGAAACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGACACCTCCCA14740.11260745073233042No Hit
GTCTGTAAACTCTTCACGACTTCCATCATGCCCGATAAAGGACACCCACTCCAGCCCCTTGCCTGGAGCCTGGCG14610.11161430496603442No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG14590.11146151330968118No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAAGACCTTGGG14490.11069755502791505No Hit
ATACTATTGTGCGAGAGACTCCATCCAGGTCTCCAGTGGGAGGTGTCTTGACTACTGGGGCCAGGGAACCCTGGT14440.11031557588703196No Hit
GACCATCACCAGGGTCGAAGCCGGGGATGAGGCCGACTATTATTGTCAGGTGTGGGACACTAGTCGTGATC14400.1100099925743255No Hit
GGGTTCCCTGGCCCCAGTAGTCAAGACACCTCCCACTGGAGACCTGGATGGAGTCTCTCGCACAATAGTATACAC14360.10970440926161905No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC14160.10817649269808675No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGCGACCTC14080.10756532607267381No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCGGCAAGTCCGTGACATGCCACGT14060.1074125344163206No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTT14030.10718334693179075No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC13910.10626659699367136No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGGT13850.10580822202461168No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG13810.10550263871190523No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT13810.10550263871190523No Hit
GTCCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCTTCGACCCTGGTGATGGTCAGGGTGGCCGTGTTCA13750.10504426374284552No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACAGTC13740.10496786791466893No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACAGTCGATCAC13740.10496786791466893No Hit
GATCAGCACTGAACACAGAGGACTCACTATGGAGTTTGGGCTGAGCTGGG13630.10412751380472615No Hit
GTCCAGAGACGATTCCAAGAACACGGTGTATTTGCAAATGAACAGTCTGA13610.10397472214837293No Hit
GTCTGGGGGAGGCGTTGTCCCGCCTGGGGCGTCCCTGAGACTCTCTTGTAAAACGTCTGGATTTCCCTTCAGCGT13550.10351634717931323No Hit
AGGCAGGCCAGGCCCCTATTTTGGTCGTCTATGATGATAGTGACCGGCCCTCAGGGATTCCTGACCGATTCTCTG13540.10343995135113661No Hit
GAGTTTACAGACTCCGTGAAGGGCCGATTCACCGTGTCCAGAGACGATTCCAAGAACACGGTGTATTTGCAAAT13490.10305797221025353No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCGAGCACTAGAAG13490.10305797221025353No Hit
CAATAATAGTCGGCCTCATCCCCGGCTTCGACCCTGGTGATGGTCAGGGTGGCCGTGTTCAGAGAGTTGGAGCCA13440.10267599306937047No Hit
TTTAAGAGGTGTCCAGTCTCAGGTGCAATTGGTGGAGTCTGGGGGAGGCGTTGTCCCGCCTGGGGCGTCCCTGA13380.10221761810031077No Hit
GAGTACGGGAGCTCTGGGAGAGGAGCCGAGCACTAGAAGTCGGCGGTGTTTCCATTCGGTGATCAGCACTGAACA13370.10214122227213415No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGGT13350.10198843061578093No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGC13270.10137726399036802No Hit
CTCTCGGTGTCAGTGGCCCCAGGACAGACGGCCACCATTACCTGTGGAGGAGACAACATTGGAACTAAACCCGTC13270.10137726399036802No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC13180.10068970153677848No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT13100.10007853491136556No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAACA507.081491E-493.3772670
TTTAAGT1502.764864E-972.62675570
AATGGTA3750.066.401670
ATGACTT2657.8216544E-1152.8550570
GTTATAC200.007811885350.974563
GTGGTAT2650.048.7769781
CGATTCA17700.048.3592270
GCACCGT10950.048.32308270
GGTAGAA25800.047.05055670
TTTACAA11250.047.03447370
TGGTATC2850.045.3176462
GGGGGTT3552.910383E-1143.83908570
GAGCCAT17050.042.90059770
GAGACAA27800.042.54599470
GGTATCA7950.041.931091
GACAGGT2200.041.7463261
GTATCAA20600.037.6482541
CCACCTT11450.035.33928370
TATCAAC22200.034.7537352
AAGCGGT12350.034.024170
Produced by FastQC (version 0.11.5)