FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686631
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAGGGGAAGCCCCTAAGGTCCTCATCTATGCTGCCTCCATTTTGCAAAGTGAAGTCCCATCAAGATTCAG36270.5282313207530682No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT26820.39060281286455173No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24010.3496783570797124No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG19080.27787851116538576No Hit
GTAGGAGACAGAGTGACCATCACTTGCCGGGCAAGTCAGACCATTGGAAA17600.2563239935278192No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT17530.25530452309901536No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17300.25195483454723133No Hit
ATCATAGACTTCGCCAAGCCATTCCAGGCCCTTCCCTGGAGTCTGGCGGACCCAACTCCACCAGGCATTATTTT16570.2413232143611343No Hit
CATCTATGCTGCCTCCATTTTGCAAAGTGAAGTCCCATCAAGATTCAGTGGCAGTGGATCTGGGACAGATTTCA15960.23243925776727237No Hit
GTCTATGATAGTGGCGTCAGCTCCTACAATTCGTCCCTCAAGAGTCGAGT15870.231128510073096No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15820.2304003169096647No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15680.22836137605205709No Hit
CCTTAGGGGCTTCCCCTGCCCTGTGCTGATACCAATTCAAAAAATTTCCAATGGTCTGACTTGCCCGGCAAGTGA14950.21772975586596002No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14720.21438006731417603No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14610.21277804235462716No Hit
CCCCTGCCCTGTGCTGATACCAATTCAAAAAATTTCCAATGGTCTGACTTGCCCGGCAAGTGATGGTCAC14570.21219548782388212No Hit
CTGTGACTGTGTTGACAGAAATAAGTGGCAAAATCTTCAGGTTGAAGACTGCTGATGGTGAGAGTGAAATCTGTC14260.20768069021060803No Hit
GGACTAAGGTTGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC13950.20316589259733392No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC13750.2002531199436087No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13560.19748598592256977No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13490.19646651549376595No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12820.18670872710378647No Hit
GTGCTGATACCAATTCAAAAAATTTCCAATGGTCTGACTTGCCCGGCAAGTGATGGTCACTCTGTCTCCTACAGA12680.1846697862461788No Hit
CAGTCACAGTGTCCCGTGGACATTCGGCCAAGGGACTAAGGTTGAAGTCAAACGAACTGTGGCTGCACCA12630.18394159308274752No Hit
GTCTCCAACCTCCCTGTCTGCATCTGTAGGAGACAGAGTGACCATCACTTGCCGGGCAAGTCAGACCATTGGAAA12550.18277648402125743No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12480.18175701359245358No Hit
GCATAGATGAGGACCTTAGGGGCTTCCCCTGCCCTGTGCTGATACCAATTCAAAAAATTTCCAATGGTCTGACT12350.1798637113675322No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC12330.17957243410215967No Hit
GTCACAGTGTCCCGTGGACATTCGGCCAAGGGACTAAGGTTGAAGTCAAACGAACTGTGGCTGCACCATCTGTCT11780.17156230930441532No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG11760.17127103203904281No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT11750.17112539340635655No Hit
GTCCTGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC11720.17068847750829777No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT11610.16908645254874888No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11590.16879517528337637No Hit
GTATCAGCACAGGGCAGGGGAAGCCCCTAAGGTCCTCATCTATGCTGCCT11370.16559112536427864No Hit
GCACAGGGCAGGGGAAGCCCCTAAGGTCCTCATCTATGCTGCCTCCATTTTGCAAAGTGAAGTCCCATCAAGAT11080.16136760501637706No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGGTG11060.16107632775100453No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT10980.15991121868951444No Hit
GTGCAGCCACAGTTCGTTTGACTTCAACCTTAGTCCCTTGGCCGAATGTC10770.15685280740310298No Hit
GTGAAGTCCCATCAAGATTCAGTGGCAGTGGATCTGGGACAGATTTCACT10490.15277492568788767No Hit
CCCCTAAGGTCCTCATCTATGCTGCCTCCATTTTGCAAAGTGAAGTCCCATCAAGATTCAGTGGCAGTGGATC10410.15160981662639758No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCAACCTTAGTCCCTTGGCCGAATGTCCACGGGACA10280.14971651440147618No Hit
GTCTCCTACAGATGCAGACAGGGAGGTTGGAGACTGGGTCAGCTGAATGTCACACCTGGCACCTCGGAGCCAGAG10210.14869704397267236No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAATCTTGATGGGACTTCACTTTGCAAAATGGAGGCAGCATAGA10190.14840576670729985No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGGTGTGACAT10120.147386296278496No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGGTGTGACATTCAGCTGACCCAGTC10050.1463668258496922No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9950.1449104395228296No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9610.13995872601149673No Hit
GCCCTGTGCTGATACCAATTCAAAAAATTTCCAATGGTCTGACTTGCCCGGCAAGTGATGGTCAC9570.13937617148075165No Hit
GATTTCACTCTCACCATCAGCAGTCTTCAACCTGAAGATTTTGCCACTTATTTCTGTCAACACAGTCACAGTGTC9370.13646339882702646No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9370.13646339882702646No Hit
CACCTGCACTGTCTCCGGTGGATACATCAAAAATAATGCCTGGTGGAGTTGGGTCCGCCAGACTCCAGGGAAGGG9070.13209423984643864No Hit
CCTTAGTCCCTTGGCCGAATGTCCACGGGACACTGTGACTGTGTTGACAGAAATAAGTGGCAAAATCTTCAGGT9010.13122040805032106No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8990.13092913078494856No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8990.13092913078494856No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8980.1307834921522623No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8970.13063785351957602No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8960.13049221488688975No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA8750.1274338036004783No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8740.12728816496779202No Hit
GTCTTCAACCTGAAGATTTTGCCACTTATTTCTGTCAACACAGTCACAGTGTCCCGTGGACATTCGGCCAAGGGA8710.12685124906973325No Hit
CACTGTGACTGTGTTGACAGAAATAAGTGGCAAAATCTTCAGGTTGAAGACTGCTGATGGTGAGAGTGAAA8630.12568614000824316No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8620.12554050137555686No Hit
GTGTGACATTCAGCTGACCCAGTCTCCAACCTCCCTGTCTGCATCTGTAG8580.12495794684481185No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8570.12481230821212558No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8460.12321028325257671No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8440.12291900598720418No Hit
GTCTCCGGTGGATACATCAAAAATAATGCCTGGTGGAGTTGGGTCCGCCAGACTCCAGGGAAGGGCCTGGAA8420.12262772872183167No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA8360.12175389692571412No Hit
GTCTGCATCTGTAGGAGACAGAGTGACCATCACTTGCCGGGCAAGTCAGACCATTGGAAATTTTTTGAATTGGTA8250.12015187196616522No Hit
CAATAATATACGGCCGTGTCCGCGGCGGTCACAGAACTCAGTCTCAAGGAGAAATGGTTCGTGGACTTGTCGA8210.1195693174354202No Hit
GTCCCGTGGACATTCGGCCAAGGGACTAAGGTTGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC8210.1195693174354202No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8120.11825856974124385No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7860.11447196529140106No Hit
GATGAGGACCTTAGGGGCTTCCCCTGCCCTGTGCTGATACCAATTCAAAA7860.11447196529140106No Hit
ATAATATACGGCCGTGTCCGCGGCGGTCACAGAACTCAGTCTCAAGGAGAAATGGTTCGTGGACTTGTCGACTGA7840.11418068802602854No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7810.11374377212796975No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG7790.11345249486259723No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT7690.11199610853573462No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC7670.11170483127036211No Hit
CTCCAGGGAAGGGCCTGGAATGGCTTGGCGAAGTCTATGATAGTGGCGTC7630.11112227673961705No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT7610.11083099947424453No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7460.10864641998395061No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG7330.10675311775902924No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT7200.10485981553410784No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG7180.10456853826873531No Hit
CCACGGGACACTGTGACTGTGTTGACAGAAATAAGTGGCAAAATCTTCAGGTTGAAGACTGCTGATGGTGAGAG7180.10456853826873531No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7120.10369470647261776No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7120.10369470647261776No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC7050.10267523604381393No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7020.10223832014575514No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG6960.10136448834963759No Hit
ATATTATTGTGTGAGAGGAGAGTTTCAGTGGTTGGGGCGCTTTGACTTCTGGGGCCGGGGGACCCCGGTCACCG6920.10078193381889253No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG6890.10034501792083375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGA550.00527774856.2722270
TATCCAA550.00527774856.2722270
TCCACGA6800.054.61715770
GCTATAA200.006050593754.37341765
GTACACG200.00650569753.3857262
TGTTAGA6900.050.83529370
GCTTATC200.00795102150.7448231
GTGGTAT900.045.106511
ATGCCGT2500.041.22660444
GTAATAC751.0550139E-940.595861
TCGTATG2450.040.51880340
GTATCAA12150.040.0946771
TTATACA2550.039.7824752
CGTATGC2500.039.74945441
AATACAC702.6082489E-838.6486473
GATCTCG2050.038.2200136
TATACAC2750.038.121623
GTATGCC2700.038.10793342
GCCGTCT2650.037.68022546
CTTATAC2700.037.588761
Produced by FastQC (version 0.11.5)