FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686631
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAGGGGAAGCCCCTAAGGTCCTCATCTATGCTGCCTCCATTTTGCAAAGTGAAGTCCCATCAAGATTCAGTG34360.5004143419099925No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26140.38069938584188595No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24600.3582710364082018No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA21480.31283178301008835No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18480.2691401932042101No Hit
CCCCTGCCCTGTGCTGATACCAATTCAAAAAATTTCCAATGGTCTGACTTGCCCGGCAAGTGATGGTCACT17460.2542850526702115No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT17360.2528286663433489No Hit
GTCTATGATAGTGGCGTCAGCTCCTACAATTCGTCCCTCAAGAGTCGAGTCACCATGTCAGTCGACAAGTCCAC17090.24889642326081984No Hit
GTAGGAGACAGAGTGACCATCACTTGCCGGGCAAGTCAGACCATTGGAAATTTTTTGAATTGGTATCAGCACAGG16720.24350779385142823No Hit
CATCTATGCTGCCTCCATTTTGCAAAGTGAAGTCCCATCAAGATTCAGTGGCAGTGGATCTGGGACAGATTTCA16580.24146885299382057No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC15880.2312741487057823No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15530.22617679656176315No Hit
ATCATAGACTTCGCCAAGCCATTCCAGGCCCTTCCCTGGAGTCTGGCGGACCCAACTCCACCAGGCATTATTTTT15030.21889486492745008No Hit
CCTTAGGGGCTTCCCCTGCCCTGTGCTGATACCAATTCAAAAAATTTCCAATGGTCTGACTTGCCCGGCAAGT14470.2107391014970195No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC14230.20724377431254926No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA13580.1977772631879423No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13540.19719470865719727No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13430.19559268369764835No Hit
CTGTGACTGTGTTGACAGAAATAAGTGGCAAAATCTTCAGGTTGAAGACTGCTGATGGTGAGAGTGAAATCTGTC13110.19093224745168802No Hit
GGACTAAGGTTGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC12720.18525234077692385No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC12630.18394159308274752No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA12540.18263084538857116No Hit
GTGCTGATACCAATTCAAAAAATTTCCAATGGTCTGACTTGCCCGGCAAGTGATGGTCACTCTGTCTCCTACA12390.18044626589827722No Hit
GTCACAGTGTCCCGTGGACATTCGGCCAAGGGACTAAGGTTGAAGTCAAA12010.17491199785619932No Hit
GTCTCCAACCTCCCTGTCTGCATCTGTAGGAGACAGAGTGACCATCACTT11890.1731643342639642No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGGTGT11660.1698146457121802No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11530.1679213434872588No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11410.16617367989502368No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG11350.1652998480989061No Hit
GCATAGATGAGGACCTTAGGGGCTTCCCCTGCCCTGTGCTGATACCAATTCAAAAAATTTCCAATGGTCTGACTT11190.16296962997592596No Hit
CCCCTAAGGTCCTCATCTATGCTGCCTCCATTTTGCAAAGTGAAGTCCCATCAAGATTCAGTGGCAGTGGATCTG11110.16180452091443587No Hit
GTCCTGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC11040.16078505048563202No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT10750.15656153013773047No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10550.15364875748400522No Hit
CAGTCACAGTGTCCCGTGGACATTCGGCCAAGGGACTAAGGTTGAAGTCAAACGAACTGTGGCTGCACCAT10520.15321184158594645No Hit
GCACAGGGCAGGGGAAGCCCCTAAGGTCCTCATCTATGCTGCCTCCATTTTGCAAAGTGAAGTCCCATCAAGATT10510.1530662029532602No Hit
CAATAATATACGGCCGTGTCCGCGGCGGTCACAGAACTCAGTCTCAAGGAGAAATGGTTCGTGGACTTGTC10380.1511729007283388No Hit
GTATCAGCACAGGGCAGGGGAAGCCCCTAAGGTCCTCATCTATGCTGCCT10370.15102726209565254No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCT10260.14942523713610367No Hit
GTCTCCTACAGATGCAGACAGGGAGGTTGGAGACTGGGTCAGCTGAATGT10060.14651246448237845No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9960.14505607815551586No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9890.14403660772671203No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGGTGTGACATTCAGCTGACCCAGTCTCCAACCTCCCTGTCTGCATCTGTA9830.14316277593059445No Hit
GTGAAGTCCCATCAAGATTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTTCAACCT9740.1418520282364181No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCAACCTTAGTCCCTTGGCCGAATGTCCACGGGACA9710.14141511233835932No Hit
GCCCTGTGCTGATACCAATTCAAAAAATTTCCAATGGTCTGACTTGCCCGGCAAGTGATGGTCACT9630.14025000327686923No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA9610.13995872601149673No Hit
GTGCAGCCACAGTTCGTTTGACTTCAACCTTAGTCCCTTGGCCGAATGTC9520.13864797831732037No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG9240.13457009660210506No Hit
GATTTCACTCTCACCATCAGCAGTCTTCAACCTGAAGATTTTGCCACTTATTTCTGTCAACACAGTCACAGTGTC9240.13457009660210506No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9220.13427881933673252No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9140.13311371027524244No Hit
CACCTGCACTGTCTCCGGTGGATACATCAAAAATAATGCCTGGTGGAGTTGGGTCCGCCAGACTCCAGGGAAGG9110.13267679437718366No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA9000.1310747694176348No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAATCTTGATGGGACTTCACTTTGCAAAATGGAGGCAGCATA8960.13049221488688975No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8910.12976402172345847No Hit
GTCTTCAACCTGAAGATTTTGCCACTTATTTCTGTCAACACAGTCACAGTGTCCCGTGGACATTCGGCCAAGG8880.12932710582539966No Hit
GTCCTCATCTATGCTGCCTCCATTTTGCAAAGTGAAGTCCCATCAAGATT8870.1291814671927134No Hit
ATAATATACGGCCGTGTCCGCGGCGGTCACAGAACTCAGTCTCAAGGAGAAATGGTTCGTGGACTTGTCGACT8660.12612305590630193No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC8480.12350156051794924No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8430.12277336735451792No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG8420.12262772872183167No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8370.12189953555840037No Hit
GTCTCCGGTGGATACATCAAAAATAATGCCTGGTGGAGTTGGGTCCGCCAGACTCCAGGGAAGGGCCTGGAATG8340.12146261966034158No Hit
CACTGTGACTGTGTTGACAGAAATAAGTGGCAAAATCTTCAGGTTGAAGACTGCTGATGGTGAGAGTGAAAT8230.11986059470079272No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8200.11942367880273394No Hit
GTCTGCATCTGTAGGAGACAGAGTGACCATCACTTGCCGGGCAAGTCAGA8140.11854984700661636No Hit
GTGTGACATTCAGCTGACCCAGTCTCCAACCTCCCTGTCTGCATCTGTAGGAGACAGAGTGACCATCACTTGCCG8040.11709346067975375No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG8030.1169478220470675No Hit
CCTTAGTCCCTTGGCCGAATGTCCACGGGACACTGTGACTGTGTTGACAGAAATAAGTGGCAAAATCTTCAGGTT7920.11534579708751863No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAG7740.11272430169916593No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7710.11228738580110714No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7400.10777258818783306No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG7390.1076269495551468No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7250.10558800869753915No Hit
GTCCCGTGGACATTCGGCCAAGGGACTAAGGTTGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC7230.10529673143216663No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT7160.10427726100336279No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7110.10354906783993148No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT7090.10325779057455897No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC7070.10296651330918645No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGGTGTGACATTCAGCTGACCCAGTCTCCAACCTCC7060.1028208746765002No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT6940.10107321108426505No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG6910.10063629518620627No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA6910.10063629518620627No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAAA200.0059359744153.8396970
AATGGTA2000.076.91984670
CATCTAA700.002816345565.931370
TAAGTTT750.003701800261.53587370
ATGGTTT800.00477971557.68988470
TATCCCG200.007221513451.995150
TTGCCGT250.00821673750.32324268
AGTTGAA11450.048.36881370
TGTCCAT2553.6325218E-948.26343570
CCAGAGT5900.046.93414770
GAGCCAT8050.043.95419770
TGGAAAT14350.041.8100970
AAGGACA13650.041.70013470
GTTAGAA350.001422833538.99418338
AAGGGTA451.1380297E-437.5079086
GTATCAA11800.036.657941
GTGGTAT1850.036.5340921
TATTTTT6950.035.80105269
CCCTCGT601.0454818E-535.7791664
CCAACCA5451.8189894E-1233.8729670
Produced by FastQC (version 0.11.5)