FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004685314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences652037
Sequences flagged as poor quality0
Sequence length19-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG33950.5206759739094561No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTCTTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTC29700.45549562371460517No Hit
GTAATACACAGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGAAGATACA27340.41930135866522916No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG22660.347526290685958No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAAGACCATTCAGGCTGTCATCCCA20860.31992049530931527No Hit
CAGTAATACACAGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGAAGATACAGCGTGTTCTTGGAATTGTCTC20080.30795798397943674No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17770.2725305465794119No Hit
GTGTATTACTGTGTGAAGGGGAGTTACGATTTTTGGAGTGGTTATTCAAACTTTGACTACTGGGGCCAGGGAACC17340.2659358287949917No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG16460.25243966216641084No Hit
GTCTTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGT15690.2406305163664025No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC15670.24032378530666204No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTG15230.23357570199237157No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT14540.22299348043132522No Hit
GTATTACTGTGTGAAGGGGAGTTACGATTTTTGGAGTGGTTATTCAAACTTTGACTACTGGGGCCAGGGAACC13910.21333145204950024No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAAGACCATTC13520.20735019638456098No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12840.19692134035338488No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCA12740.19538768505468246No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12240.18771940856117061No Hit
AGTACATACTACGCAGACTCCGTGAAGGGCAGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCT12100.1855722911429873No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11800.18097132524688017No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11490.1762169938209028No Hit
CCACAAGACCATTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGA11420.17514343511181113No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG10850.1664015999092076No Hit
CTCCTGTTCAGCCTCTGGATTCACCTTCAGTAACTATGCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGA10790.16548140672998618No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10730.16456121355076475No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10680.16379438590141357No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10670.16364102037154332No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT10660.1634876548416731No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10620.16287419272219214No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC10530.16149390295336No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10530.16149390295336No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10480.16072707530400882No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10480.16072707530400882No Hit
GTATGTACTACCCCCATTTCTACTAATAGCTGAAACATATTCCAGTCCCTTCCCTGGAGCCTGGCGGACCCAG10210.1565862059975124No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10180.1561261094079017No Hit
GCACTGAAGACAGAAGACTCATCATGGAGTTCTGGCTGAGCTGGGTTCTC10130.15535928175855052No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9960.15275206775075648No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC9720.1490712950338708No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG9600.14723090867542793No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC9580.14692417761568743No Hit
CCCCTGAGGGCCGCTGATTATTAGTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCAGTTTA9540.1463107154962065No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG9190.1409429219507482No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG9160.1404828253611375No Hit
GCATGGGATGACAGCCTGAATGGTCTTGTGGTATTCGGCGGAGGGACCAA9110.1397159977117863No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGTTTGAATAAC9110.1397159977117863No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGA9060.13894917006243512No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT8970.13756888029360295No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGTTTGAATAACCACTCCAAAAATCGTAACTCCCCTTCACACAGTAATACA8890.13634195605464108No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC8890.13634195605464108No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8880.13618859052477084No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8850.13572849393516012No Hit
GTCTGAGAGCTGAGGACACGGCTGTGTATTACTGTGTGAAGGGGAGTTACGATTTTTGGAGTGGTTATTCAAACT8850.13572849393516012No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAG8570.13143425909879347No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAG8530.1308207969793125No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8520.1306674314494423No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8420.1291337761507399No Hit
CATCTATACTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC8340.12790685191177803No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT8240.12637319661307564No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC8220.12606646555333517No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8180.1254530034338542No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC8160.12514627237411374No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA8010.12284578942606018No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC8000.12269242389618994No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAAGACCAT7980.12238569283644946No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT7970.12223232730657922No Hit
GAGTACGGGAGCTCTGGGAGAGGAGCCCCAGGCCCGGGATTCCCAGGTGT7890.12100540306761733No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC7890.12100540306761733No Hit
GATTATTAGTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCAGTTTACAGAATTACTTCCGA7880.12085203753774709No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA7870.12069867200787684No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7790.11947174776891496No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTCTGTAAACTGGTACCAGCACCTCCCAGGAACGGCCCC7680.11778472694034235No Hit
GTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCAGTTTACAGAATTACTTCCGATGTTGGAG7650.11732463035073162No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAAT7600.11655780270138044No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG7570.11609770611176974No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCCAGGCCCGGGATTCCCAGGTGTTTCCATTCAGTGA7530.11548424399228877No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC7520.11533087846241855No Hit
GTACGGGAGCTCTGGGAGAGGAGCCCCAGGCCCGGGATTCCCAGGTGTTT7480.11471741634293758No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAAGACCATTCA7470.11456405081306735No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG7470.11456405081306735No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTCT7400.1134904921039757No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7310.11211020233514354No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7240.11103664362605191No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG7190.11026981597670071No Hit
CCCTGAGACTCTCCTGTTCAGCCTCTGGATTCACCTTCAGTAACTATGCT7160.10980971938709No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGACTGGAATATGTTTC7090.10873616067799832No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7050.10812269855851739No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG7010.10750923643903644No Hit
CTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCA6990.10720250537929596No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAGCA6960.10674240878968526No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC6890.10566885008059358No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG6890.10566885008059358No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA6790.10413519478189122No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC6770.10382846372215074No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC6760.10367509819228049No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTGATTA6740.10336836713254002No Hit
ATACTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCC6680.10244817395331861No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC6640.10183471183383766No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC6610.10137461524422693No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT6560.10060778759487575No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCC6560.10060778759487575No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)