FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004685316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1199630
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCGCACCTCGAACGTTCGGCCAAGGGACCCAGGTGGAAATCAAACGAACTG66320.5528371247801405No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT44530.37119778598401176No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41730.34785725598726275No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA38120.31776464409859706No Hit
GTATGGTAGCGCACCTCGAACGTTCGGCCAAGGGACCCAGGTGGAAATCAAACGAACTGTGGCTGCACCA37290.31084584413527505No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT33310.2776689479256104No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA31480.2624142443920209No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC31240.2604136275351567No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT29730.24782641314405274No Hit
GTAGTACACAGCCGTGTCCTCGACTCTGAGACTGTTCATTTGAAGATATA29100.24257479389478426No Hit
GTGTATTACTGTCAGCAGTATGGTAGCGCACCTCGAACGTTCGGCCAAGGGACCCAGGTGGAAATCAAACGAA29030.2419912806448655No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25950.2163166976484416No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25610.21348249043455064No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25300.21089836032776774No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACC24540.20456307361436443No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24160.2013954302576628No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23730.19781099172244773No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC23420.19522686161566483No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA23170.19314288572309796No Hit
GTGTACTACTGTGCACGGCCAGTGGGAACTTCCGTGCTTGTTGACTACTGGGGCCAGGGAACCCTGGTCACCG22610.18847477972374815No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22550.1879746255095321No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22310.1859740086526679No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA22290.18580729058126255No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21500.17922192676075124No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTA21410.17847169543942715No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCC21270.1773046689395897No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC20910.1743037436542934No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGCACCATAGATGAGGAGCC20260.16888540633361954No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20100.16755166176237674No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT19240.16038278469194667No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG19140.15954919433491993No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCTGCTAACACTCTGA19100.15921575819210923No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC17660.14721205705092402No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTGGGTCC16880.14071005226611538No Hit
ACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGC16560.13804256312362978No Hit
GTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTA16520.13770912698081908No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16020.13354117519568534No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA15910.1326242258029559No Hit
GTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCA15790.1316239173745238No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15710.1309570450889024No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15680.13070696798179438No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC15670.1306236089460917No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGCACCATAGA15610.13012345473187567No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15520.1293732234105516No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTGGGTCCCTTGGCCGAACGTT15440.1287063511249302No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCGCA14670.1222877053758242No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14620.12187091019731085No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14590.12162083309020282No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14510.12095396080458141No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14410.12012037044755465No Hit
CAGTAGTACACAGCCGTGTCCTCGACTCTGAGACTGTTCATTTGAAGATATAGTGTGTTCTTGGAGTTGTCTC14360.1197035752690413No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA14320.1193701391262306No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14030.116952727090853No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC13950.11628585480523161No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGG13150.10961713194901762No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12990.10828338737777482No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA12970.10811666930636947No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12920.10769987412785609No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT12890.10744979702074806No Hit
GTACTACTGTGCACGGCCAGTGGGAACTTCCGTGCTTGTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC12870.1072830789493427No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12840.1070330018422347No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC12650.10544918016388387No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12390.10328184523561432No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG12280.1023648958428849No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT12260.10219817777147955No Hit
CTCCTGTGTAGCCTCTGAATTCACCTTCAGCACCTACTGGATGCACTGGGTCCGCCAAGCTCCAGGGAAGGGG12170.10144794645015547No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)