FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1460871
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGATGGTGCGCTCACCTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAACTG81710.5593238554259753No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT59150.4048954356681733No Hit
GTATGATGGTGCGCTCACCTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCT55160.3775829624929237No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48610.3327466970047321No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA44850.3070086270450984No Hit
CCATCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC44030.3013955373198592No Hit
GCATATAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTC38340.2624461708117965No Hit
GTATTTAGCCTGGTACCAGCTGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATGGTGCATATAACAGGGCCA37670.2578598657923937No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA36350.24882416038103294No Hit
GTGTATTACTGTCAGCAGTATGATGGTGCGCTCACCTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAACTG34370.23527060226399182No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT33350.22828846626430396No Hit
GTTATATGCACCATAAATGAGGAGCCTGGGAGCCTGGCCAGGTTTCAGCT32330.2213063302646161No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32180.22027954555877965No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31700.216993834500103No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC29280.20042837457927495No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28900.19782718665782265No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27140.18577957944267495No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26400.18071410822721512No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26340.18030339434488055No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGTAAGTATTTAGCCTGGTACC26110.17872899112926466No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26050.17831827724693008No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25590.1751694708156983No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTA25580.17510101850197587No Hit
CCCTTAGACTCTCCTGTGTAGCCTCTGGATTCAGTTTCACTGACGTCTGG25350.17352661528636No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC24540.16798197787484315No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23810.16298495897310578No Hit
GTGTTAGTAGTAAGTATTTAGCCTGGTACCAGCTGAAACCTGGCCAGGCT23590.1614790080712123No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAGTAAGTATTTAGCC23290.15942543865953943No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA22890.15668734611064222No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT22670.15518139520874877No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTTATATGCACCATAAATGAGGAGCC22460.15374389662057772No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG22190.15189568415007212No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21660.14826771152278334No Hit
CTCCTGTGTAGCCTCTGGATTCAGTTTCACTGACGTCTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG20590.1409433139544833No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG20250.1386159352879207No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT19850.13587784273902348No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGACTGAGCTGGATTGTC19810.13560403348413377No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT19770.13533022422924407No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC19720.13498796266063193No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19350.132455227052902No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA19330.13231832242545713No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19130.13094927615100854No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC18700.12800582666094407No Hit
CATTTATGGTGCATATAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA18620.12745820815116463No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA18340.12554154336693657No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC18040.12348797395526367No Hit
GAGCTGGATTGTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCT17890.12246118924942723No Hit
GTCCTGGTGTCTCTCAGCGTAGGCATCCGGCCCCTCTGTGGCGCAATAAT17800.12184511842592537No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17300.11842250273980386No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17070.11684809952418798No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGATGGTGCG17040.11664274258302068No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTTATATGCACCATAAA16890.11561595787718422No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC16880.1155475055634618No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16660.11404155466156833No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGCGACAGTCGGGGG16430.11246715144595246No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16410.11233024681850759No Hit
GTCAGAGTGTTAGTAGTAAGTATTTAGCCTGGTACCAGCTGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTA15970.10931834501472068No Hit
GAGTTTGGACTGAGCTGGATTGTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAG15790.10808620336771693No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15760.10788084642654963No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15680.10733322791677019No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15460.10582727701487674No Hit
CACCAGGACTGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGT15390.10534811081881973No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA15380.10527965850509731No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCAC15330.10493739693648516No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT15290.10466358768159542No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC15180.1039106122306487No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC15130.10356835066203655No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCCCGCCGAAGGTG15020.10281537521108983No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC14960.10240466132875525No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT14780.10117251968175149No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)