FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1460871
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGATGGTGCGCTCACCTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAACTGTG71860.4918983264093818No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57750.39531211174703307No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA50980.348969895356948No Hit
GTATGATGGTGCGCTCACCTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT48630.332883601632177No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA42410.2903062624968255No Hit
CCATCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC42220.2890056685360993No Hit
GTATTTAGCCTGGTACCAGCTGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATGGTGCATATAACAGGGCCA38900.2662795003802526No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35370.24211583363623482No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA35230.24115750124412083No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT33320.22808310932313666No Hit
GCATATAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTC32270.22089561638228153No Hit
GTGTATTACTGTCAGCAGTATGATGGTGCGCTCACCTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAACTGTG31310.21432419426492827No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31250.21391348038259367No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC30920.21165455402975347No Hit
GTTATATGCACCATAAATGAGGAGCCTGGGAGCCTGGCCAGGTTTCAGCT29770.20378253795167406No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29220.20001766069694038No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC28610.19584206955987216No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC25070.17160995050213196No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA24800.16976173803162634No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA24480.16757126399250857No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGTAAGTATTTAGCCTGGTACCAGCTGAAACCTGGCCAGGCTCCCAG24190.1655861468945581No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23760.1626426974044936No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23710.16230043583588147No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAGTAAGTATTTAGCCTG23580.1614105557574899No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT23110.15819329701253568No Hit
CCCTTAGACTCTCCTGTGTAGCCTCTGGATTCAGTTTCACTGACGTCTGGATGAGCTGGGTCCGCCAGGCTCCAG23010.15750877387531137No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT22970.15723496462042164No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA22020.1507319948167908No Hit
GTGTTAGTAGTAAGTATTTAGCCTGGTACCAGCTGAAACCTGGCCAGGCT21890.14984211473839923No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA21730.14874687771884032No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC21270.14559807128760857No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTTATATGCACCATAAATGAGGAGCCTG20840.14265462179754407No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20610.14108021858192818No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20450.1399849815623693No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20190.13820522140558614No Hit
CTCCTGTGTAGCCTCTGGATTCAGTTTCACTGACGTCTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGG20030.13710998438602726No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA19260.13183915622940012No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC18370.12574690030810387No Hit
GAGCTGGATTGTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTT18070.12369333089643096No Hit
CATTTATGGTGCATATAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA17790.12177666611220292No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17760.12157130917103563No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAG17430.11931238281819544No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17160.11746417034768984No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGATGGTGC17020.11650583795557581No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGACTGAGCTGGATTGTC16640.11390465003412346No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC16460.11267250838711974No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16280.11144036674011601No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGG16190.11082429591661412No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGTCTGGGCACTGTGTGGCC16120.11034512972055713No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA16070.11000286815194496No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCACCAGGCACCCTGTCTTTGTCTCCA15960.10924989270099823No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA15960.10924989270099823No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15920.10897608344610853No Hit
GTCCTGGTGTCTCTCAGCGTAGGCATCCGGCCCCTCTGTGGCGCAATAATACACGGCTGTGTCCTCGATTTTCAG15800.10815465568143937No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT15560.10651180015210104No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCCCGCCGAAGGTG15340.10500584925020758No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15310.10480049230904029No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15300.10473203999531787No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15130.10356835066203655No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCAC15100.10336299372086927No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15050.10302073215225711No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTTCAGGGCCAGCTCCTCCGACGGCGGC14910.1020623997601431No Hit
TAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCA14650.10028263960335992No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA150.003238491188.4191370
CGTTTAA404.2806272E-4105.9857670
AATGGTA5550.066.2013270
ACTACTA17000.057.35770
TAATCGC200.00790423150.823544
GGTATCA11050.044.8228721
GAGCAAA23750.043.43556670
AAGCGGT16000.041.5110970
GTATCAA30250.040.260411
ACCGTTT806.9040107E-736.04980568
ATGGCAA3952.564775E-1035.77578770
TATCAAC35650.034.1234662
ATAAACG601.51959175E-533.882363
ATCAACG36300.033.3223233
CAGCCAT16650.033.1006670
TCAACGC36700.033.0514564
CATTTAA2152.472636E-432.86380470
GAGGCTA8650.032.6738470
AACGCAG37850.032.5854726
CAACGCA37350.032.4762655
Produced by FastQC (version 0.11.5)