FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences661341
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT24980.3777173954132588No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24470.3700057912635085No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAAGCTGTCCATCTGCAGAAACA20130.3043815520283787No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17990.27202305618432854No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT16750.25327327354572No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG15660.23679160977468508No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15330.23180174826602312No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15130.228777589775925No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14550.22000753015464034No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14180.21441283694795873No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14000.21169109430687044No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13580.20534036147766432No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC12980.19626788600736988No Hit
GTATATTACTGTGCGAAAGAGACGGGGAACTATGGTTTCCATGATTACTTTGACTACTGGGGCCAGGGAGCCC12800.19354614336628154No Hit
GTGGGGTCCCCTCAAGGTTCAGTGGCAGTGGATCTGGGACCGATTTCACT12700.19203406412123247No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12410.18764903431059016No Hit
ACTTACTACTGTCAACAAAGTTACAGTAACCCCCTTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTG12090.1828103807264331No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT11770.17797172714227608No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG11250.17010891506802087No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGGATTAGCAG11200.16935287544549635No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGGATTAGCAGCTATTTAAATTGGTA10610.1604316078997068No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10510.15891952865465772No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC10090.15256879582545163No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC9990.15105671658040254No Hit
AAGTTACAGTAACCCCCTTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCT9930.15014946903337312No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTG9920.1499982611088682No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9800.14818376601480931No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9800.14818376601480931No Hit
GTAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCGGTCCCAGATCCACTGCCA9590.14500839960020626No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACA9510.14379873620416697No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9130.1380528350729805No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT8860.13397022111134801No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATG8720.1318533101682793No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8380.12671224073511245No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8210.12414170601852902No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8160.12338566639600448No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8150.12323445847149958No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG8020.12126875545293578No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAAGG7950.12021029998140141No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7930.1199078841323916No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7890.11930305243437198No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7840.11854701281184744No Hit
ACCTTGAGGGGACCCCACTGTGCAAATTAAATGAAGCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTC7810.11809338903833273No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7740.11703493356679837No Hit
ACCACATACTACGCAGACTCCGTGAAGGGCCGCTTCACCATCTCCAGAGACGAGTCCAAGAACATGGTGTTTC7680.11612768601976893No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7660.11582527017075911No Hit
ATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTCTGCAACTTACTACTGTCAACAAAGTTACAGTAACC7610.11506923054823456No Hit
GTTCAGTGGCAGTGGATCTGGGACCGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTCTGCAACTTA7490.11325473545417569No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA7400.1118938641336315No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGGATTAGCAGCTATTTAAATTGGTATCAGCAGAAA7320.11068420073759226No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7030.10629917092694993No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT6990.10569433922893032No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC6900.10433346790838613No Hit
CCTCAAGGTTCAGTGGCAGTGGATCTGGGACCGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTC6880.10403105205937632No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG6860.10372863621036651No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC6760.10221655696531745No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC6720.1016117252672978No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT6670.10085568564477326No Hit
GGACCCCACTGTGCAAATTAAATGAAGCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAAT6660.10070447772026836No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)