FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004685322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003627
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT40470.40323745774077424No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38260.3812173247630843No Hit
GTATAACAGTTATTTTTCGGGGTACAGTTTTGGCCAGGGGACCAAGCTGG27470.27370726375436294No Hit
GCGTATTAGTAGCTCGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCG27060.269622080713253No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT26610.265138343229108No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26330.2623484621278622No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC26240.2614517146310333No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGCGTATTAGTAGCTCGTTGGCCTGGTA25090.24999327439377378No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA24300.24212182414383032No Hit
CTGTTATACTGTTGGCAGTAATAAGTTGCACAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC23350.23265615612174645No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22840.22757458697304872No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22830.22747494836228999No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22100.22020132977689919No Hit
CCTTCCACCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGCGTATTAGTAGCTCG21790.21711253284337706No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21450.21372482007757862No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20780.2070490331567405No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC20480.20405987483397714No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20310.20236601845107796No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19800.1972844493023803No Hit
GGCCAAAACTGTACCCCGAAAAATAACTGTTATACTGTTGGCAGTAATAAGTTGCACAATCATCAGGCTGCAGG18800.18732058822650247No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT18770.18702167239422615No Hit
CAGTATAACAGTTATTTTTCGGGGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCA18520.1845307071252567No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18510.18443106851449792No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTGTGCAACTTATTACTGCCAACAGTATAACAGTTAT18400.18333504379615134No Hit
GTTATACTGTTGGCAGTAATAAGTTGCACAATCATCAGGCTGCAGGCTGC18240.18174082602401093No Hit
ACTTATTACTGCCAACAGTATAACAGTTATTTTTCGGGGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAA18180.18114299435945824No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACA17850.17785492020441857No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16910.16848889079309345No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGCGTATTAGTAGCTCGTTGGCCTGGTATCAGCAGAAA16830.16769178190702322No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC16320.16261021275832555No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15880.15822611388493932No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTGTACCCCGAAAAATAACTGTTATA15830.15772792083114542No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTG15320.15264635168244775No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGG15000.14945791613816686No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT14940.1488600844736142No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14360.1430810450496051No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGG14320.14268249060656996No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC14320.14268249060656996No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14080.1402911639483593No Hit
GTGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTCG13900.1384976689547013No Hit
GTAATAAGTTGCACAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG13570.13520959479966163No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13560.13510995618890284No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACATCCAGATGACCCAGTCTCCTTCCACC13040.1299287484294464No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13020.12972947120792883No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12980.12933091676489372No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12890.12843416926806472No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12380.12335260011936705No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12230.12185802095798538No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGCGTATTAGTAG12080.12036344179660372No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGAGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCAC11820.11777283791687548No Hit
GTATTACTGTGCGAGACCTCGGGACTTTGATACTACTGGTCCTTTTAATTACTGGGGCCAGGGAACCCTGGTCAC11750.11707536764156404No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGAGGTCCCA11540.11498295681562971No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11290.11249199154666026No Hit
CCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACGAGCTACTAATACGCTGA11200.11159524404983126No Hit
GATCTATAAGGCGTCTAGTTTAGAAAGTGAGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA11160.11119668960679614No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCTCACTTTCTAAACTAGACGCCTTATAGA11020.10980174905617326No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10910.1087057243378267No Hit
GTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG10860.1082075312840328No Hit
GTCTGGAGCAGAGGTGAAAAAGCCGGGGGAGTCTCTGAAGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAG10780.10741042239796258No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10740.10701186795492748No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT10610.10571656601506337No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT10500.1046205412967168No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG10440.10402270963216413No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA10310.10272740769230002No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10170.10133246714167712No Hit
ATGTATTACTGTGCGAGACCTCGGGACTTTGATACTACTGGTCCTTTTAA10040.10003716520181301No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)