FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003627
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39340.3919782947250323No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA34720.3459452565544769No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30940.30828186168765886No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27650.27550075874802094No Hit
GTATAACAGTTATTTTTCGGGGTACAGTTTTGGCCAGGGGACCAAGCTGG27070.2697217193240118No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT26290.2619499076848271No Hit
CTGTTATACTGTTGGCAGTAATAAGTTGCACAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC23710.23624314610906244No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC23560.23474856694768076No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC23180.23096229973884722No Hit
GCGTATTAGTAGCTCGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGT21960.2188063892262763No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA21700.21621578534654806No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC20920.2084439737073634No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20560.2048569837200474No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCT20560.2048569837200474No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGC20530.20455806788777106No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20000.19927722151755584No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC19330.1926014345967177No Hit
CCTTCCACCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCG18880.1881176971125727No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTGTGCAACTTA18350.18283685074235748No Hit
GGCCAAAACTGTACCCCGAAAAATAACTGTTATACTGTTGGCAGTAATAAGTTGCACAATCATCAGGCTGCAG17890.17825347464745367No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA17620.17556323215696668No Hit
GTTATACTGTTGGCAGTAATAAGTTGCACAATCATCAGGCTGCAGGCTGC17260.17197624216965068No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17200.17137841050509803No Hit
ACTTATTACTGCCAACAGTATAACAGTTATTTTTCGGGGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAA17180.17117913328358045No Hit
CAGTATAACAGTTATTTTTCGGGGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCA17160.1709798560620629No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16580.16520081663805378No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA16260.1620123810937729No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16230.16171346526149655No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15920.15862466832797442No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15800.1574290049988691No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCTCACTTTCTAAACTAGACGCCTTATA15630.15573514861596988No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACAT15600.15543623278369353No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGCGTATTAGTAGCTCGTTGGCCTGGTATCAGCAGAAA15100.15045430224575465No Hit
GTAATAAGTTGCACAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCC15020.14965719335968442No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT14820.14766442114450887No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGT14670.14616984198312719No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGGCGGTATTGATGGACTTGTCGACTGA14620.14567164892933332No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTGTACCCCGAAAAATAACTGTTATA14620.14567164892933332No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA14620.14567164892933332No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13510.13461176313510897No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC13190.1314233275908281No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13050.1300283870402052No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12830.12783633760351207No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12760.12713886732820062No Hit
GTGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCG12130.12086163485039761No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT11480.11438512515107706No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAA11290.11249199154666026No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGCGTATTAGTA11180.11139596682831371No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACATCCAGATGACCCAGTCTCCTTCCACC11170.11129632821755492No Hit
GTATTACTGTGCGAGACCTCGGGACTTTGATACTACTGGTCCTTTTAATTACTGGGGCCAGGGAACCCTGGTCAC11150.11109705099603738No Hit
CCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACGAGCTACTAATACGCT11100.11059885794224347No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGAGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT10840.10800825406251525No Hit
GTCTGGAGCAGAGGTGAAAAAGCCGGGGGAGTCTCTGAAGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCA10740.10701186795492748No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10720.10681259073340992No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10640.1060154818473397No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10540.10501909573975192No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10390.10352451657837025No Hit
GATCTATAAGGCGTCTAGTTTAGAAAGTGAGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA10330.10292668491381758No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC10330.10292668491381758No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACATCCAGAT10300.10262776908154125No Hit
GTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG10250.10212957602774736No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10200.10163138297395347No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCAAT1002.0090575E-457.98798470
CACGCTT200.00827401350.23786524
CTATCGG200.00834445150.13023810
GAGGGTT1751.0878593E-649.70398770
TATTCTA1204.942198E-448.3233270
AATCGAA1204.942198E-448.3233270
TGCACCA20800.048.09099270
AATGGTA3353.274181E-1143.27461670
CTGGTAT16150.042.189470
GAGCAAA22000.040.85516770
CTCGTAT2154.4866865E-640.4567370
AAGCGGT5500.039.53726270
GTAGTTT2001.3898389E-436.2424970
ATGGCAA2101.8515793E-434.51665570
CGTCACA11000.034.26562570
TAGGCAA5101.8189894E-1234.1105870
GATTCAC1400.033.460171
GTGTATA502.2278128E-433.4601671
GGTCAAA2601.6516527E-533.45460570
TATCAAC27850.032.5250172
Produced by FastQC (version 0.11.5)